Biocompatible silk random lasing is obtained by nanostructuring proteins into a disordered porous matrix via self-assembly technique. Lasing action revealed spectral narrowing and threshold behavior, it sensitive to pH variations in the aqueous environment. Silk provides versatile biocompatible system, opening up opportunities for biophotonic applications biosensing. Lasers made from biological materials have potential produce new coherent light sources, flexible compatible living tissue...

Cellulose is the most abundant biopolymer on Earth. fibers, such as one extracted form cotton or woodpulp, have been used by humankind for hundreds of years to make textiles and paper. Here we show how, engineering light–matter interaction, can optimize light scattering using exclusively cellulose nanocrystals. The produced material sustainable, biocompatible, when compared ordinary microfiber-based paper, it shows enhanced strength (×4), yielding a transport mean free path low 3.5 μm in...

Here we report a random lasing based sensor which shows pH sensitivity exceeding by 2-orders of magnitude that conventional fluorescence sensor. We explain the sensing mechanism as related to gain modifications and threshold nonlinearities. A dispersive diffusive theory matches well experimental results, allow us predict optimal conditions maximal large 200 times an identical fluorescence-based The simplicity operation high make it promising for future biosensing applications.

Biolasers obtained from biomaterials are attracting a wealth of interest for their potential as future biosensors with enhanced sensitivity, and advanced cell tracking. Here, miniature biolasers reported, which formed by bovine serum albumin (BSA) protein biosourced polysaccharides derived land plants such cellulose pectin. Using green processing route aided simple emulsions, solid‐state microspheres diameters 15–100 µm fabricated utilized whispering gallery mode lasers thresholds few µJ mm...

Abstract Multiplexed, specific, and sensitive detection of antigens is critical for the rapid accurate diagnosis disease informed development personalized treatment plans. Here, it shown that polymer microsphere lasers can be used as photonic sensors to monitor quantify direct surface binding biomolecules via changes in refractive index. The unique spectral signature each individual laser find their size effective index which adds a new encoding dimension when compared conventional...

Nucleic acid sensing is crucial for advancing diagnostics, therapeutic monitoring and molecu-lar biology research, by enabling the precise identification of DNA RNA interactions. Here, we present an innovative platform based on DNA-functionalized whispering gallery mode (WGM) microlasers. By correlating spectral shifts in laser emission to changes refractive index, demonstrate real-time detection hybridization structural changes. The addition gold nanoparticles strands significantly enhances...

Nucleic acid sensing is crucial for advancing diagnostics, therapeutic monitoring, and molecular biology research by enabling the precise identification of DNA RNA interactions. Here, we present an innovative platform based on DNA-functionalized whispering gallery mode (WGM) microlasers. By correlating spectral shifts in laser emission to changes refractive index, demonstrate real-time detection hybridization structural changes. The addition gold nanoparticles strands significantly enhances...

Biointegrated intracellular microlasers have emerged as an attractive and versatile tool in biophotonics. Different inorganic semiconductor materials been used for the fabrication of such biocompatible but often operate at visible wavelengths ill-suited imaging through tissue. Here, we report on whispering gallery mode microdisk lasers made from a range GaInP/AlGaInP multi-quantum well structures with compositions tailored to red-shifted excitation emission. The selected alloys show minimal...

Self-assembled fluorescent particles have shown promise as a potential structure for random lasers. However, obtaining micron-sized lasers made with remains challenge. Theoretically, achieving could be possible by assembling supraparticles composed of colloidal particles. Despite extensive research on supraparticles, the generation from this is rarely reported. In study, we introduce rapid and efficient method producing The resulting exhibit diameters ranging 50 to 150 µm well-connected...

The miniaturization of random lasers to the micrometer scale is challenging but fundamental for integration with photonic integrated circuits and biological tissues. Herein, it demonstrated that a diameter from 30 160 μm can be achieved by using simple emulsion process selective chemical etching. These tiny laser sources are made either dye‐doped polyvinyl alcohol (PVA) or bovine serum albumin (BSA) they in form microporous spheres monodisperse pores 1.28 diameter. Clear lasing action...

Flexible and tensile wavelength-tunable micrometer-sized random lasers in the form of microporous polymer fiber are demonstrated. The fibers fabricated by directly drawing from an aqueous mixture subsequently selective chemical etching a green solvent. Size-dependent lasing threshold spectrum investigated. Owing to mechanical flexibility, wavelength-tuning 5.5 nm is achieved stretching fiber.

Random Micro-Biolasers Micro-sized random lasers made with organic materials are interesting for many photonic applications, but their fabrication is challenging. In article number 2100036, Van Duong Ta, Riccardo Sapienza, and co-workers demonstrate a versatile technique fabricating micro-scale diameters ranging from 30 to 160 μm. The synthesized dye-doped polyvinyl alcohol or bovine serum albumin have microporous structure pores of 1.28

ABSTRACT The estimation of full-field displacement between biological image frames or in videos is important for quantitative analyses motion, dynamics and biophysics. However, the often weak signals, poor contrast many noise processes typical to microscopy make this a formidable challenge contemporary methods. Here, we present deep-learning method, termed Displacement Estimation FOR Microscopy (DEFORM-Net), that outperforms traditional digital correlation optical flow methods, as well...

Whispering gallery mode microlasers are known for their high Q-factors, characteristic emission spectra and sensitivity to local refractive index changes. This combined with the ability of various cell types internalise these provide unique opportunities advanced biological studies, e.g. in single-cell tracking intracellular sensing. Despite many advancements, achieving precise delivery lasers cells remains challenging, traditional methods, such as microinjection, often also resulting...

Intracellular lasers are emerging as powerful biosensors for multiplexed tracking and precision sensing of cells their microenvironment. This capacity is enabled by quantifying narrow-linewidth emission spectra, which presently challenging to do at high speeds. In this work, we demonstrate rapid snapshot hyperspectral imaging intracellular lasers. Using integral field mapping with a microlens array diffraction grating, obtain images the spatial spectral intensity distribution from single...

Fluorescent probes are widely used in biological imaging; however, their spectral properties often limit sensing and multiplexing. Instead, intracellular lasers, offer increased purity, photostability distinct outputs enabling the unique tagging of multiple cells over long time periods. Here we report on optimisation low threshold miniature 1000-fold smaller than eukaryotic nucleus (Vlaser<0.1μm³). The improved fabrication method has allowed us to explore more complex laser...

Microsphere solid-state biolasers made of biomaterials, which include bovine serum albumin, pectin, and cellulose, are demonstrated in article number 1601022 by Van Duong Ta co-workers. The lasers compatible with cell growth division, as the cover image where a 30 μm biolaser is incubated HeLa cells.

On page 998, biocompatible silk random lasing is achieved in natural nanostructured as a disordered porous matrix via self-assembly techniques by F. G. Omenetto, R. Sapienza, and co-workers. Lasing action sensitive to pH variations the aqueous environment opening up opportunities for biophotonic applications biosensing.

Abstract Integrating laser particles into live cells, tissue cultures, and small animals is an emerging rapidly evolving technique that offers non-invasive interrogation labelling with unprecedented information density. The bright distinct spectra of make this approach particularly attractive for high-throughput applications requiring single-cell specificity, such as multiplexed cell tracking intracellular biosensing. To be practical relevance, the implementation these requires...

Intracellular lasers are emerging as powerful biosensors for multiplexed tracking and precision sensing of cells their microenvironment. This capacity is enabled by quantifying narrow-linewidth emission spectra, which presently challenging to do at high speeds. In this work, we demonstrate rapid snapshot hyperspectral imaging intracellular lasers. Using integral field mapping with a microlens array diffraction grating, obtain images the spatial spectral intensity distribution from single...

We have constructed a hyperspectral confocal microscope for high-speed and high-resolution readout of spectra from bio-integrated microlasers. demonstrate its advanced performance in multiplexed cell tracking dynamic sensing experiments.