A5055120012- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Genetic factors in colorectal cancer
- SARS-CoV-2 and COVID-19 Research
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Tuberculosis Research and Epidemiology
- BRCA gene mutations in cancer
- Dermatological and COVID-19 studies
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Diverticular Disease and Complications
- Colorectal Cancer Treatments and Studies
- Microbial Community Ecology and Physiology
- Diagnosis and treatment of tuberculosis
- Microbial Fuel Cells and Bioremediation
- Bacterial Infections and Vaccines
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Appendicitis Diagnosis and Management
Mater Dei Hospital
2025
Hebrew University of Jerusalem
2016-2023
Small RNAs (sRNAs) associated with the RNA chaperon protein Hfq are key posttranscriptional regulators of gene expression in bacteria. Deciphering sRNA-target interactome is an essential step toward understanding roles sRNAs cellular networks. We developed a broadly applicable methodology termed RIL-seq (RNA interaction by ligation and sequencing), which integrates experimental computational tools for vivo transcriptome-wide identification interactions involving Hfq-associated sRNAs. By...
We have previously described the existence of membranous nanotubes, bridging adjacent bacteria, facilitating intercellular trafficking nutrients, cytoplasmic proteins, and even plasmids, yet components enabling their biogenesis remain elusive. Here we reveal identity a molecular apparatus providing platform for nanotube biogenesis. Using Bacillus subtilis (Bs), demonstrate that conserved flagellar export (FliO, FliP, FliQ, FliR, FlhB, FlhA), designated CORE, dually serve flagellum assembly....
Bacterial small RNAs (sRNAs) are posttranscriptional regulators of gene expression that base pair with complementary sequences on target mRNAs, often in association the chaperone Hfq. Here, using experimentally identified sRNA-target pairs, along measurements, we assess basic principles regulation by sRNAs. We show sRNA sequence dictates repertoire, as point mutations shift set correspondingly. distinguish two subsets targets: targets showing changes levels under overexpression their...
Within-host adaptation is a hallmark of chronic bacterial infections, involving substantial genomic changes. Recent large-scale data from prolonged infections allow the examination adaptive strategies employed by different pathogens and open door to investigate whether they converge toward similar strategies. Here, we compiled extensive whole-genome sequences isolates belonging miscellaneous species sampled at sequential time points during clinical infections. Analysis these revealed that...
Intramuscularly administered vaccines stimulate robust serum neutralizing antibodies, yet they are often less competent in eliciting sustainable “sterilizing immunity” at the mucosal level. Our study uncovers a strong temporary component of immunity, emanating from intramuscular administration an mRNA vaccine. We show that saliva BNT162b2 vaccinees contains IgA targeting receptor-binding domain (RBD) severe acute respiratory syndrome coronavirus-2 spike protein and demonstrate these IgAs...
Abstract Background Crohn’s disease (CD) complicated with intra-abdominal abscess often requires surgical intervention. Image guided percutaneous drainage (PD) can help to avoid interventions, however there is limited evidence on the optimal management after PD. Our study aimed analyze long-term outcomes of CD Methods In this multicenter, multinational retrospective trial penetrating patients simplex were enrolled and followed. Baseline was defined as day first detection abdominal abscess,...
Mucinous colorectal carcinomas (MC) constitute 10% of malignancies. Recently, an increased risk cancer has been demonstrated in germline BRCA1/2 mutation carriers. Furthermore, carriers have exhibited a higher-than-expected frequency MC tumors. Here, we investigate the relationship between BRCA mutations and mucinous histology carcinoma patients, using both existing cohort sequenced tumors prospective case-control study comparing conventional adenocarcinoma (AC) patients tested for...
Bacteria adapt to their host by mutating specific genes and reprogramming gene expression. Different strains of a bacterial species often mutate the same during infection, demonstrating convergent genetic adaptation. However, there is limited evidence for adaptation at transcriptional level. To this end, we utilize genomic data 114 Pseudomonas aeruginosa strains, derived from patients with chronic pulmonary P. regulatory network. Relying on loss-of-function mutations in encoding regulators...
Coinfection of HIV and multidrug-resistant tuberculosis (MDR-TB) presents significant challenges in terms the treatment prognosis tuberculosis, leading to complexities managing disease impacting overall outcome for TB patients. This study a remarkable case patient with MDR-TB coinfection who survived over 8 years, despite poor adherence comorbidities. Whole genome sequencing (WGS) infecting Mycobacterium (Mtb) strain revealed unique genomic deletion, spanning 18 genes, including key genes...
Abstract Intramuscularly administered vaccines stimulate robust serum neutralizing antibodies, yet they are often less competent in eliciting sustainable ‘sterilizing immunity’ at the mucosal level. Our study uncovers, strong component (NT50 ≤ 50pM), emanating from intramuscular administration of an mRNA vaccine. We show that saliva BNT162b2 vaccinees contains temporary IgA targeting Receptor-Binding-Domain (RBD) SARS-CoV-2 spike protein and demonstrate these IgAs key mediators potent...
Abstract Coinfection of HIV and multidrug-resistant tuberculosis (MDR-TB) presents significant challenges in terms the treatment prognosis tuberculosis, leading to complexities managing disease impacting overall outcome for TB patients. This study a remarkable case patient with MDR-TB coinfection who survived over eight years, despite poor adherence comorbidities. Whole genome sequencing (WGS) infecting Mycobacterium ( Mtb ) strain revealed unique genomic deletion, spanning 18 genes,...