A5070829373- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Nanofabrication and Lithography Techniques
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Cellular transport and secretion
- Innovative Microfluidic and Catalytic Techniques Innovation
- Hematological disorders and diagnostics
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- RNA Research and Splicing
- Polymer Surface Interaction Studies
- Religion, Spirituality, and Psychology
- Cardiomyopathy and Myosin Studies
- Hemoglobinopathies and Related Disorders
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Neuroscience and Neural Engineering
- RNA Interference and Gene Delivery
- Dendrimers and Hyperbranched Polymers
- Signaling Pathways in Disease
- Biotechnology and Related Fields
- Science Education and Perceptions
- Renal and related cancers
- Clinical Laboratory Practices and Quality Control
- Advanced Surface Polishing Techniques
University of Wisconsin–Madison
2014-2025
Wisconsin Institutes for Discovery
2014-2025
Madison Group (United States)
2015
Human pluripotent stem cell (hPSC)-derived neural organoids display unprecedented emergent properties. Yet in contrast to the singular neuroepithelial tube from which entire central nervous system (CNS) develops vivo, current organoid protocols yield tissues with multiple units, a.k.a. rosettes, each acting as independent morphogenesis centers and thereby confounding coordinated, reproducible tissue development. Here, we discover that controlling initial morphology can effectively (>80%)...
Significance The endoplasmic reticulum (ER) serves as a platform for the packaging of most secretory proteins into conserved coat protein complex II (COPII)-coated transport carriers destined ER–Golgi intermediate compartments (ERGIC) in animal cells. In this work, we demonstrate that Trk-fused gene (TFG), implicated multiple neurodegenerative diseases and oncogenesis, functions pathway by interacting directly with COPII Sec23. Specifically, show TFG outcompetes interactions between inner...
The 5th International Conference on Developmental Neurotoxicity (DNT) Testing (DNT5) took place in April 2024 Konstanz, Germany, organized by CAAT-Europe, the University of and scientists from US EPA, SCAHT, CAAT at Johns Hopkins Bloomberg School Public Health. conference convened experts regulatory agencies, industry, academia to explore latest advancements DNT testing integration animal-free new approach methodologies (NAMs) into next-generation risk assessment (NGRA). key topic was...
<italic>In situ</italic> regulation of the morphology neural tissues derived from human pluripotent stem cells using micropatterned, clickable substrates.
Recent advances in stem cell biology have allowed researchers to efficiently produce large numbers of cardiomyocytes from various pluripotent sources. Unfortunately, these cells exhibit properties that are characteristics immature such as poor sarcomere organization, limited calcium handling, and reduced size alignment. Specifically, the actin–myosin motor proteins form sarcomeres within fail large, highly ordered repeating structures distinctive for adult myocytes. Instead, heterogeneous...
SUMMARY Neural organoids have revolutionized how human neurodevelopmental disorders (NDDs) are studied. Yet, their utility for screening complex NDD etiologies and in drug discovery is limited by a lack of scalable quantifiable derivation formats. Here, we describe the RosetteArray ® platform’s ability to be used as an off-the-shelf, 96-well plate assay that standardizes incipient forebrain spinal cord organoid morphogenesis micropatterned, 3-D, singularly polarized neural rosette tissues...
Increased realization of the spatial heterogeneity found within in vivo tissue microenvironments has prompted desire to engineer similar complexities into vitro culture substrates. Microcontact printing (μCP) is a versatile technique for engineering such onto cell substrates because it permits microscale control relative positioning molecules and cells over large surface areas. However, challenges associated with precisely aligning superimposing multiple μCP steps severely limits extent...
In tissue engineering, it is desirable to exhibit spatial control of morphology and cell fate in culture on the micron scale. Culture substrates presenting grafted poly(ethylene glycol) (PEG) brushes can be used achieve this task by creating microscale, non-fouling adhesion resistant regions as well where cells participate biospecific interactions with covalently tethered ligands. To engineer complex tissues using such substrates, will necessary sequentially pattern multiple PEG...
Abstract Autism spectrum disorder (ASD) is a genetically heterogeneous linked with rare, inherited and de novo mutations occurring in two main functional gene categories: expression regulation synaptic function 1 . Accumulating evidence points to dysregulation cortical neurogenesis as convergent mechanism ASD pathophysiology 2-8 While asynchronous development has been identified shared feature among ASD-risk genes the category of regulation, it remains unknown whether this phenotype also...
Abstract Embryonic stem cells (ESCs) can self-organize in vitro into developmental patterns with spatial organization and molecular similarity to that of early embryonic stages. This self-organization ESCs requires transmission signaling cues, via addition small molecule chemicals or recombinant proteins, induce distinct cellular fates subsequent assembly structures mimic aspects development. During natural development, different cell types co-develop together, where each type expresses...
Abstract Human pluripotent stem cell (hPSC)-derived neural organoids have revolutionized in vitro modelling of human neurological disorders. Cell-intrinsic morphogenesis processes displayed within these tissues could serve as the basis for ex vivo manufacture brain and spinal cord with biomimetic anatomy physiology. However, we must first understand how to control their emergent properties starting at genesis organoid formation, i.e. emergence polarized neuroepithelium. In vivo, all CNS...
ABSTRACT Reprogramming of human somatic cells to induce pluripotent stem (iPSCs) generates valuable precursors for disease modeling and regenerative medicine. However, the reprogramming process can be inefficient noisy, creating many partially reprogrammed in addition fully iPSCs. To address these shortcomings, we developed a micropatterned substrate that allows dynamic live-cell microscopy thousands cell subpopulations undergoing reprogramming. Micropatterning facilitated change shape, size...
Abstract Autism spectrum disorder (ASD) is a genetically heterogeneous linked with rare, inherited and de novo mutations occurring in two main functional gene categories: expression regulation synaptic function1. Accumulating evidence points to dysregulation cortical neurogenesis as convergent mechanism ASD pathophysiology. While asynchronous development has been identified shared feature among ASD-risk genes the category of regulation, it remains unknown whether this phenotype also...
In tissue engineering, it is desirable to exhibit spatial control of morphology and cell fate in culture on the micron scale. Culture substrates presenting grafted poly(ethylene glycol) (PEG) brushes can be used achieve this task by creating microscale, non-fouling adhesion resistant regions as well where cells participate biospecific interactions with covalently tethered ligands. To engineer complex tissues using such substrates, will necessary sequentially pattern multiple PEG...