Despite the poor genomics research capacity in Africa, efforts have been made to empower African scientists get involved research, particularly that involving populations. As part of Human Heredity and Health Africa (H3Africa) Consortium, an initiative was set make endeavor developed through funding from United States' National Institutes Common Fund Wellcome Trust. H3Africa is intended encourage a contemporary approach by investigators stimulate study genomic environmental determinants...

Mycobacterium tuberculosis (MTB) clinical strains are relatively varied at the genome level. This in-silico study analyzed genomic differences between MTB isolates from blood and lungs of TB-HIV positive cohorts in Uganda. The hypothesis was that have distinct SNPs INDELs make them better survivors. Twenty-four MTB-blood -lung sequences were aligned against H37Rv reference using BWA-MEM, IGV, SAMtools, FreeBayes, SnpEff. Comparative analysis revealed had 11 virulence genes with distinctive...

Between January 2015 and July 2017, we investigated the frequency of carbapenem resistant Acinetobacter baumannii (CRAB) Pseudomonas aeruginosa (CRPA) at Mulago Hospital intensive care unit (ICU) in Kampala, Uganda. Carbapenemase production carbapenemase gene carriage among CRAB CRPA were determined; mobility potential genes via horizontal transfer processes was also studied.Clinical specimens from 9269 patients processed for isolation CRPA. Drug susceptibility testing performed with disk...

Face-masking could reduce the risk of COVID-19 transmission. We assessed knowledge, attitudes, perceptions, and practices toward face-mask use among 644 high-risk individuals in Kampala, Uganda. In data analysis, descriptive, bivariate, multivariate logistic regression analyses with a 95% CI were considered. Adjusted odds ratios used to determine magnitude associations. P-values < 0.05 considered statistically significant. The majority, 99.7% 87.3% participants, respectively, had heard about...

Introduction Escherichia coli , Klebsiella pneumoniae and Enterobacter (EKE) are the leading cause of mortality morbidity in neonates Africa. The management EKE infections remains challenging given global emergence carbapenem resistance Gram-negative bacteria. This study aimed to investigate source organisms for maternity environment a national referral hospital Uganda, by examining phenotypic molecular characteristics isolates from mothers, neonates, ward. Methods From August 2015 2016, we...

Despite the immense potential of genomics and bioinformatics in revolutionising public health low-income countries (LICs) Africa, their complete integration faces multiple obstacles. Among these obstacles, scarcity expertise, as underscored by Harris Onywera colleagues, is notable.1Onywera H Ondoa P Nfii F et al.Boosting pathogen workforce Africa.Lancet Infect Dis. 2023; (published online Sept 28)https://doi.org/10.1016/S1473-3099(23)00394-8PubMed Google Scholar commendable efforts...

Abstract Background Lab-based surveillance (LBS) of antimicrobial resistance (AMR) is not systematically implemented in Uganda. We aimed to identify the gaps establishing regular LBS AMR Methods This was a retrospective records review. It done at Mulago Hospital (MH) Microbiology Lab (MHL). analyzed lab on bacteria and their susceptibility profiles (ASPs) over 6 months. Since MH national referral hospital, we hypothesized that (1) MHL best resourced any limitations seen here are amplified...

Antimicrobial Resistance (AMR) and Healthcare Associated Infections (HAIs) are major global public health challenges in our time. This study provides a broader updated overview of AMR trends surgical wards Mulago National Referral Hospital (MNRH) between 2014 2018. Laboratory data on the antimicrobial susceptibility profiles bacterial isolates from 428 patient samples were available. The most common as follows: tracheal aspirates (36.5%), pus swabs (28.0%), blood (20.6%). Klebsiella (21.7%),...

Abstract In Uganda, the challenge of generating and timely reporting essential antimicrobial resistance (AMR) data has led to overreliance on empirical antibiotic therapy, exacerbating AMR crisis. To address this issue, study aimed adapt a one-step testing protocol alongside an SMS (Short Message Service) result relay system (SRRS), with potential reduce turnaround time for communication from 4 days or more 1 day in Ugandan clinical microbiology laboratories. Out 377 samples examined, 54...

<ns4:p>Bacterial infections involving antibiotic-resistant gram-negative bacteria continue to increase and represent a major global public health concern. Resistance antibiotics in these is mediated by chromosomal and/or acquired resistance mechanisms, give rise multi-drug resistant (MDR), extensive-drug (XDR) or pan-drug (PDR) bacterial strains. Most recently, plasmid-mediated colistin, an antibiotic that had been set apart as the last resort treatment of MDR, XDR PDR has reported....

Reliable data on antimicrobial resistance (AMR) transmission dynamics in Uganda remains scarce; hence, we studied this area. Eighty-six index patients and "others" were recruited. Index those who had been admitted to the orthopedic ward of Mulago National Referral Hospital during study period; included medical non-medical caretakers patients, patients' immediate hospital neighbors. Others recruited only when became positive for carrying antimicrobial-resistant bacteria (ARB) their stay. A...

Poor adherence to malaria treatment guidelines among healthcare workers (HCWs) is a major contribution diagnostic challenges, failure, and non-rational use of antimalarial medicines. However, there limited information about HCWs in private health facilities informal settlements Uganda. This study therefore assessed the level associated factors Kampala's settlements. A cross-sectional was conducted 339 from slums 4 selected divisions Kampala, Quantitative data collected using semi-structured...

Background: Non-prescribed use of antimicrobials in Agriculture incurs a transfer risk resistant pathogens to humans, complicating treatment. The aim this study was determine the potential Zoonotic E. coli serve as drivers antimicrobial resistance (AMR) among animals and humans pastoralist communities Kasese district, so protect community.&#x0D; Materials Methods: A laboratory based cross-sectional done using archived isolates previously obtained from Uganda. Recovery by conventional...

The dual burden of road traffic accidents and antimicrobial resistance in orthopaedic infections is challenging already strained health-care systems. Limited information exists Tanzania on surveillance to delineate the potential sources multi-drug-resistant bacteria for specific mitigation strategies among patients.A longitudinal study was conducted at Bugando Medical Centre Mwanza city between January May 2020. It involved collection rectal swabs/stools, hand swabs, environmental sampling...

Antimicrobial resistance remains a threat to human health globally.1Antimicrobial Resistance CollaboratorsGlobal burden of bacterial antimicrobial in 2019: systematic analysis.Lancet. 2022; 399: 629-655Summary Full Text PDF PubMed Scopus (302) Google Scholar Despite serious gaps the data, marked contribution made by African low-income and middle-income countries (LMICs) number deaths due cannot be ignored.1Antimicrobial Indeed, LMICs Africa should given even greater attention than ever...

Abstract Face-masking could reduce the risk of COVID-19 transmission. We assessed: knowledge, attitudes, perceptions, and practices towards face-mask use among 644 high risk-individuals in Kampala, Uganda. In data analysis, descriptive, bivariate multivariate logistic regression analyses, with a 95% confidence interval were considered. Adjusted-odds ratios used to determine magnitude associations. P-values &lt; 0.05 considered statistically-significant. Majority: 99.7% 87.3% participants...

With shortages of face masks being reported worldwide, it is critical to consider alternatives commercially manufactured masks. This study aimed test and compare the efficacy various makes locally made or homemade cloth obtained from face-mask vendors in Kampala, Uganda, during COVID-19 pandemic. The testing was performed assess bacterial filtration efficiency (BFE), breathability, distance-dependent fitness, reusability masks, while considering most commonly used non-published...

Abstract The high global bacterial infection burden has created need to investigate the neglected potential drivers of pathogenic bacteria, inform disease prevention. Kampala is facing a proliferation herbalists, selling herbal medicine (HM), largely unregulated microbiological quality. We evaluated contamination in HM sold Kampala, support evidence-based redress. total viable loads (TVL), coliform counts (TCC), E. coli counts, and prevalence selected strains 140 were examined using...

Isoniazid preventive therapy (IPT) is given to HIV patients reduce the risk of active tuberculosis (TB). However, treatment completion remains suboptimal among those that are initiated. This study aimed determine level IPT and factors associated with non-completion on antiretroviral (ART) at Kisenyi Health Center IV in Kampala, Uganda.A mixed-methods facility-based retrospective cohort utilizing routinely collected data from 341 randomly selected initiated was conducted. Data extracted...

<ns4:p>Bacterial infections involving antibiotic resistant gram-negative bacteria continue to increase and represent a major global public health concern. Resistance antibiotics in these is mediated by chromosomal and/or acquired resistance mechanisms, give rise multi-drug (MDR) or extensive drug (XDR) bacterial strains. Most recently, novel plasmid mechanism colistin, an that had been set apart as the last resort treatment of MDR XDR bacteria, has reported. Plasmid colistin have described...

<ns3:p>COVID-19 control is highly affected by knowledge levels which are also receipt of information on the disease. Therefore, this review examined and media sources findings studies knowledge, attitudes, perceptions, practices towards COVID-19 done in low- middle-income countries Africa to shed light interplay use different platforms populations’ about pandemic identify shorter- longer-term priorities for research improve continent’s capacity not only deal with but future pandemics....