A5003922609- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Hedgehog Signaling Pathway Studies
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Genomic variations and chromosomal abnormalities
- Cancer-related molecular mechanisms research
- Congenital Ear and Nasal Anomalies
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- melanin and skin pigmentation
- RNA regulation and disease
- Pancreatitis Pathology and Treatment
- Genetic and phenotypic traits in livestock
- RNA Research and Splicing
- Circular RNAs in diseases
- Cancer-related Molecular Pathways
- Phagocytosis and Immune Regulation
- Genetic Mapping and Diversity in Plants and Animals
- Ferroptosis and cancer prognosis
- interferon and immune responses
- Dietary Effects on Health
National Institute of Biomedical Genomics
2013-2025
Regional Centre for Biotechnology
2021-2025
All India Institute of Hygiene and Public Health
2023
University of Wisconsin–Madison
2009-2023
Institute of Post Graduate Medical Education and Research
2023
University of Wisconsin Carbone Cancer Center
2010-2014
George Mason University
2012
Kanazawa University
2009
Indian Institute of Chemical Biology
2003-2006
Achieving accurate genomic estimated breeding values for dairy cattle requires a very large reference population of genotyped and phenotyped individuals. Assembling such populations has been achieved breeds as Holstein, but is challenging with fewer An alternative to use multi-breed population, that smaller gain some advantage in accuracy (GEBV) from information larger breeds. However, this marker-quantitative trait loci associations persist across Here, we assessed the GEBV Jersey result...
Intracellular parasitic protozoans of the genus Leishmania depend for their survival on elaboration enzymic and other mechanisms evading toxic free-radical damage inflicted by phagocytic macrophage host. One such mechanism may involve superoxide dismutase (SOD), which detoxifies reactive radicals produced activated macrophages, but role this enzyme in parasite has not yet been demonstrated. We have cloned a SOD gene from L. tropica generated SOD-deficient parasites expressing corresponding...
Wnt and Hedgehog signaling pathways play central roles in embryogenesis, stem cell maintenance, tumorigenesis. However, the mechanisms by which these two interact are not well understood. Here, we identified a novel mechanism pathway stimulates transcriptional output of signaling. Wnt/beta-catenin induces expression an RNA-binding protein, CRD-BP, turn binds stabilizes GLI1 mRNA, causing elevation activity. The newly described mode regulation seems to be important several functions Wnt,...
Alternative cleavage and polyadenylation generates multiple transcript variants producing mRNA isoforms with different length 3′-UTRs. enables differential post-transcriptional regulation via the availability of cis-acting elements in Microphthalmia-associated transcription factor (MITF) is a master regulator melanocyte development melanogenesis. This central also implicated melanoma development. Here, we show that cells favor expression MITF shorter 3′-UTR. We establish this isoform...
<title>Abstract</title> A complex formulized as [Cu(dmpn)<sub>2</sub>(OH<sub>2</sub>)][Cu<sub>2</sub>(DPA)<sub>4</sub>µ-Cu(dmpn)<sub>2</sub>] (<bold>1</bold>), where dmpn is 2,2<sup>׳</sup>-dimethyl-1,3-diaminepropane and DPA dipicolinic acid (H<sub>2</sub>dipic), was synthesized characterized by X-ray crystallography, conductivity measurement, thermogravimetric (TG) analysis spectroscopic methods (IR, UV-Vis). The initial <italic>in vitro</italic> cytotoxic activity of (<bold>1</bold>)...
Reports indicate a worldwide increase in the incidence of Early-Onset Colorectal Carcinoma (EOCRC) (<50 years old). In an effort to understand different modes pathogenesis early-onset CRC, colorectal tumors from EOCRC old) and Late-Onset patients (LOCRC; >50 were screened eliminate microsatellite instability (MSI), nuclear β-catenin, APC mutations, as these are known canonical factors CRC pathogenesis. Small-RNA sequencing followed by comparative analysis revealed differential expression 23...
Background The world is going through the critical phase of COVID-19 pandemic, caused by human coronavirus, SARS-CoV-2. Worldwide concerted effort to identify viral genomic changes across different sub-types has identified several strong in coding region. However, there have not been many studies focusing on variations 5' and 3' untranslated regions their consequences. Considering possible importance these host mediated regulation RNA genome, we wanted explore phenomenon. Methods To an idea...
In kinetoplastid protozoa, import of cytosolic tRNAs into mitochondria occurs through interacting with membrane-bound proteins, the identities which are unknown. The inner membrane RNA complex Leishmania tropica contains multiple proteins and is active for in vitro . RIC1, largest subunit this complex, structurally homologous to conserved α F1 ATP synthase. RIC1 gene complemented an atpA mutation Escherichia coli Antisense-mediated knockdown RIC1/F1α resulted depletion several mitochondrial...
The aggressiveness of pancreatic ductal adenocarcinoma is primarily due to lack effective early detection biomarkers. Circulating non-coding RNAs serve as diagnostic or prognostic biomarkers in multiple types cancer. Comparison their expression between diseased tissue and relevant body fluids such saliva, urine, bile, juice, blood etc. may reveal mechanistic involvement common RNAs. piwi-interacting (piRNAs) are a class aim the present study was investigate plasma tumour piRNA changes...
The mitochondrial genomes of a wide variety species contain an insufficient number functional tRNA genes, and translation mRNAs is sustained by import nucleus-encoded tRNAs. In Leishmania, transfer tRNAs across the inner membrane can be regulated positive negative interactions between them. To define factors involved in such interactions, large multisubunit complex (molecular mass, approximately 640 kDa) from kinetoplastid protozoon consisting 130-A particles, was isolated. complex, when...
RNA-binding proteins (RBPs) play a significant role in multiple cellular processes with their deregulations strongly associated cancer. However, there are not adequate evidences regarding global alteration and functions of RBPs pancreatic cancer, interrogated systematic manner. In this study, we have prepared an exhaustive list from sources, downloaded gene expression microarray data total 241 tumors 124 normal tissues, performed meta-analysis, obtained differentially expressed (DE-RBPs)...
Abstract Background The world is going through the critical phase of COVID-19 pandemic, caused by human coronavirus, SARS-CoV2. Worldwide concerted effort to identify viral genomic changes across different sub-types has identified several strong in coding region. However, there have not been many studies focusing on variations 5’ and 3’ untranslated regions their consequences. Considering possible importance these host mediated regulation RNA genome, we wanted explore phenomenon. Methods To...
Most often, the patients with pancreatic diseases are presented a mass in head region and existing methods of diagnosis fail to confirm whether is malignant or benign. As subsequent management disease hugely depends on correct diagnosis, we wanted explore possible biomarkers which could distinguish benign masses.In order address that gap, performed case-control study identify genome-wide differentially expressed coding noncoding genes between tissues collected from masses. These were next...
Import of nucleus-encoded tRNAs into the mitochondria kinetoplastid protozoon Leishmania involves recognition specific import signals by membrane-bound machinery. Multiple on different tRNA domains may be present, and further, importable RNAs interact positively (Type I) or negatively II) with one another at inner membrane in vitro. By co-transfection assays, it is shown here that tRNA(Tyr) transiently stimulates rate entry tRNA(Ile) transfected cells, conversely, inhibited tRNA(Ile)....
Import of tRNAs into the mitochondria kinetoplastid protozoon Leishmania requires tRNA-dependent hydrolysis ATP leading to generation membrane potential through pumping protons. Subunit RIC1 inner RNA import complex is a bi-functional protein that identical alpha-subunit F1F0 synthase and specifically binds subset (Type I) importable tRNAs. We show recombinant, purified Type I hydrolase. The activity was insensitive oligomycin, sensitive mutations within signal tRNA, required cooperative...
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