Brine shrimp lethality assay is an important tool for the preliminary cytotoxicity of plant extract and others based on ability to kill a laboratory cultured larvae (nauplii). The nauplii were exposed different concentrations 24 hours. number motile was calculated effectiveness extract. It simple, cost effective requires small amount test material.Video Clip Methodology:Brine Shrimp Lethality Assay: 4 min 9 sec Full Screen Alternate

Background. Millions of people in Bangladesh, India, Taiwan, and Chile are consuming high concentration arsenic through drinking water, thousands them have already developed chronic poisoning. There is no specific treatment. Some authors suggest the use vitamins minerals for more than 6 months. The present placebo-controlled double-blind study was conducted to evaluate effectiveness spirulina extract plus zinc treatment Methods. Forty-one patients poisoning were randomly treated orally by...

The change in cytoplasmic free calcium, [Ca 2+ ] i isolated bovine adrenal medullary cells during stimulation by acetylcholine (ACh) Ca ‐free incubation medium was measured using the fluorescent indicator quin2. ACh (1–100 μM) caused an increase mobilization of from intracellular pool. Nicotine (10 did not absence extracellular . Pretreatment with atropine completely inhibited ACh‐induced , whereas pretreatment hexamethonium (100 not. antagonist 8‐( N...

Water hyacinth (Eichhornia crassipes) removes arsenic from arsenic-contaminated drinking water. This effect depends on several factors, such as the amount of water hyacinth, present in water, duration exposure, and presence sunlight air. On basis study, authors suggest that is useful for making totally free. provides a natural means removing at household level without monetary cost.

Cesarean section is a common operative procedure in obstetrics. Prophylactic uses of antimicrobials different categories surgeries are irrational general, which also observed cesarean section. Cost antimicrobial imposes an enormous burden on the patient or system and considered as important cost component section.A cross-sectional study was conducted from August 2002 to July 2003 8 units Departments Gynecology & Obstetrics Bangabandhu Sheikh Mujib Medical University, Sir Salimullah...

An in vivo study was carried on rats to see the influence of arsenic aerobic gut flora. A significant inhibition flora observed after 2 weeks administration (1 mg/L) ad libitum with a decrease stool level and increase liver level. However, this inhibitory effect not presence vitamin E mg/day) or selenium (0.4 mg/day). Pretreatment streptomycin (500 mg twice daily) showed similar results. Rats that received folic acid (200 floral count but there were decreased level.Keywords: Aerobic;...

Melanosis and leucomelanosis with or without keratosis are the earliest symptoms of arsenicosis. Uneven distribution arsenical melanosis in skin led us to investigate possibility preferential secretion arsenic three constituents sweat; cholesterol, vitamin E, zinc.Twenty-four-hour was collected from lesions unaffected sites 20 patients. Skin secretions were people exposed arsenic-contaminated drinking water healthy, unexposed individuals.The healthy controls (mean ± SE; unit: μg/in.(2)...

Thousands of Bangladeshi are suffering from arsenic-induced keratosis affecting palms and soles. Palmar keratosis, particularly in young female, affects the socioeconomic condition. Till today there is no effective treatment. Twenty patients mild to moderate degree arsenical palmar were treated with garlic oil soft capsule (10 mg) daily orally for 12 weeks. Similar treatment was given 17 arsenic exposed controls 15 healthy volunteers comparison. Effectiveness evaluated by change clinical...

Keratosis, one of the earliest skin manifestations arsenicosis, can be treated by either oral or topical formulation drug. In this study we examined effectiveness and tolerance propylene glycol for treatment arsenical palmar keratosis. Sixty patients arsenicosis with palmer keratoses were randomly divided into three groups different concentrations (15, 30 45%) applied topically their palms once at bedtime eight weeks. The perception patient about progress was scored 'Likert scale'. mean...

<p>Spectrophotometric method of arsenic estimation in nails has four steps: a) washing nails, b) digestion c) generation, and finally d) reading absorbance using spectrophotometer. Although the is a cheapest one, widely used effective, it time consuming, laborious need caution while acids. </p><p><strong>Introduction</strong></p><p>Estimation total water biological samples are important for diagnosis arsenicosis (Misbahuddin, 2015). Biological...

This study was done to examine the role of Nigella sativa in 28 patients palmar arsenical keratosis. Patients were randomized into two groups: Group A (n-15) receive capsules placebo and vitamin E (200 mg) whereas B (n= 13) N. oil (500 orally for 8 weeks. The mean (± SD) clinical scoring palmer keratosis before after treatment 99.3 ± 21.5 62.3 14.3 respectively (37% reduction). On other hand, treated group, score 119.1 20.3 which reduced 39.2 8.4 (p<0.0001) (67% There 65% reduction total...

&lt;p class="Abstract"&gt;The aim of this study was to see the effectiveness &lt;em&gt;Solanum melongena&lt;/em&gt; peel extract in treatment arsenic-induced Bowen’s disease. In total 8 patients disease were selected from two arsenic-endemic areas. The containing ointment provided each patient and instructed him/her apply twice daily on site lesion for 12 weeks. measurement size as well photograph collected both before at end study. mean (± SD) diameter 3.8 ± 1.9 cm which reduced 2.5 1.7...

Spirulina, a dietary supplement, improves the symptoms of arsenical palmer keratosis. To understand its mechanism action, palm skin extracts moderate keratosis (n=10), arsenic exposed controls (n=10) and healthy volunteers were collected before after treatment with spirulina powder 10 g/day orally for 12 weeks. The mean (±SD) amount zinc in was 13.1 ± 5.7 ng/cm2, which not changed significantly patients (11.3 5.3 ng/cm2). vitamin E 6.0 0.3 ng/cm2 severely reduced (3.5 0.6 linoleic acid...

Quest is still going on for a cheap, effective, and easily available remedy chronic arsenic toxicity. This study was designed to investigate the effects of folic acid tetrahydrofolate in lowering burden tissues. Rats received at 700 µg/day by gavage 28 days except control group. Arsenic accumulation significantly lowered (p&lt;0.05), liver, kidney, heart, lung skin both tetrahydrofolate-treated groups compared only treated The oxidative stress induced treatment reduced as evident reduction...

A bioequivalence study of a local antifungal drug, fluconazole (FlunacTM), was compared with that innovator product DiflucanTM. The conducted on 15 healthy volunteers and single cross-over dose 150 mg administered orally. Two milliliter blood collected at different time intervals for 96 hours. drug extracted by liquid-liquid extraction estimated high performance liquid chromatography diode array detector (260 nm). chromatographic separation accomplished using C18 analytical column mobile...

&lt;p class="Abstract"&gt;The aim of this study was to examine the pattern fungi in palmar arsenical keratosis. The skin swab taken from five different sites (palm, dorsum, axilla, front and back chest) arsenicosis patients (n=37), arsenic exposed volunteers (n=20) healthy an endemic area. Fungi were cultured Sabouraud dextrose agar media. contained only four species fungi: Aspergillus, Dermatophytes, Mucor Fusarium. incidence each fungus low (0-4 out 20 cases). chronic exposure did not...

&lt;p&gt;The aim of the present study was to examine involvement skin bacteria in pathogenesis keratosis. Skin swab five different sites (palm, dorsum hand, chest, axilla and alar crease) from arsenic unexposed volunteer (n = 15), exposed 15) arsenicosis patients (n= 20)were collected for bacterial culture. The volunteers contained Staphylococcus epidermidis &lt;em&gt;Bacillus&lt;/em&gt; spp. Chronic exposure high concentration through drinking water altered pattern. growth...