A5060915788- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- CRISPR and Genetic Engineering
- Heat shock proteins research
- Insect and Arachnid Ecology and Behavior
- Neuroscience and Neuropharmacology Research
- Spaceflight effects on biology
- Photosynthetic Processes and Mechanisms
- RNA regulation and disease
- Photoreceptor and optogenetics research
National Brain Research Centre
2021-2024
Neurons are vulnerable to physical insults, which compromise the integrity of both dendrites and axons. Although several molecular pathways axon regeneration identified, our knowledge dendrite is limited. To understand mechanisms regeneration, we used PVD neurons in C. elegans with stereotyped branched dendrites. Using femtosecond laser, severed primary this neuron. After severing near cell body, observed sprouting new branches from proximal site within 6 hours, regrew further time an...
PVD neuron of Caenorhabditis elegans is a highly polarized cell with well-defined axonal, and dendritic compartments. operates in multiple sensory modalities including the control both nociceptive touch sensation body posture. Although axon dendrites this show regeneration response following laser-assisted injury, it rather unclear how behavior associated affected by loss these structures. It also whether neurite regrowth would lead to functional restoration neurons. Upon axotomy, using...
PVD neuron of C . elegans has become an attractive model for the study dendrite development and regeneration due to its elaborate stereotype morphology. RNA interference (RNAi) by feeding E coli expressing dsRNA been basis several genome wide screens performed using However, method often fails when it comes knocking down genes in nervous system. In order optimize RNAi conditions neuron, we fed worm strains with HT115 bacteria against mec-3 , hpo-30 tiam-1 whose loss function are known show...
Abstract Neurons are vulnerable to physical insults which compromise the integrity of both dendrites and axons. Although several molecular pathways axon regeneration identified, our knowledge dendrite is limited. To understand mechanisms regeneration, we used PVD neurons in C. elegans having stereotyped branched dendrites. Using femtosecond laser, severed primary this neuron. After severing near cell body, observed sprouting new branches from proximal site within 6 hours, regrew further with...
Abstract PVD neuron of C. elegans is a highly polarized cell with well-defined axonal, and dendritic compartments. operates in multiple sensory modalities controlling both nociceptive touch sensation body posture. Although axon dendrites this show regeneration response following laser-assisted injury, it rather unclear how the behavior associated affected by loss these structures. It also whether neurite regrowth would lead to functional restoration neurons. Upon axotomy, using femtosecond...
Abstract PVD neuron of C. elegans has become an attractive model for the study dendrite development and regeneration due to elaborate stereotype morphology in this neuron. The molecular basis maintenance is poorly understood. RNA interference (RNAi) by feeding E. coli expressing dsRNA been several genome wide screens performed using . However, method often fails when it comes nervous system. Using optimal induction condition expression E , we fed worm strains with HT115 bacteria against...