A5076354206- Endoplasmic Reticulum Stress and Disease
- Cholesterol and Lipid Metabolism
- Birth, Development, and Health
- Viral gastroenteritis research and epidemiology
- Ubiquitin and proteasome pathways
- RNA regulation and disease
- Animal Virus Infections Studies
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- Diet, Metabolism, and Disease
- Pancreatic function and diabetes
- Lipid metabolism and biosynthesis
- SARS-CoV-2 and COVID-19 Research
University of Pennsylvania
2016-2021
ABSTRACT Viruses in the family Coronaviridae , within order Nidovirales are etiologic agents of a range human and animal diseases, including both mild severe respiratory diseases humans. These viruses encode conserved replicase structural proteins as well more diverse accessory proteins, encoded 3′ ends their genomes, that often act host cell antagonists. We previously showed 2′,5′-phosphodiesterases (2′,5′-PDEs) by prototypical Betacoronavirus mouse hepatitis virus (MHV), Middle East...
Genetic variants near the TRIB1 gene are highly significantly associated with plasma lipid traits and coronary artery disease. While is likely causal of these associations, molecular mechanisms not well understood. Here we sought to investigate how influences low density lipoprotein cholesterol (LDL-C) levels in mice. Hepatocyte-specific deletion Trib1 (Trib1Δhep) mice increased apoB slowed catabolism LDL-apoB due decreased LDL receptor (LDLR) mRNA protein. Simultaneous transcription factor...
Abstract Hepatocytes store triglycerides (TGs) in the form of lipid droplets (LDs), which are increased hepatosteatosis. The regulation hepatic LDs is poorly understood and new therapies to reduce hepatosteatosis needed. We performed a siRNA kinase phosphatase screen HuH‐7 cells using high‐content automated imaging LDs. Changes accumulated lipids were quantified with developed pipeline that measures intensity, area, number Selected “hits,” reduced accumulation, further validated other...
Human genetics has proven to be a powerful approach identify novel physiological pathways and therapeutic targets for coronary artery disease (CAD), the leading cause of death worldwide. Genetic variants at chromosomal locus 8q24, 40kb from TRIB1 gene, are significantly associated with CAD, as well known CAD risk factors: plasma levels low-density lipoproteins (LDL) triglyceride (TG)-rich lipoproteins. Hepatic overexpression Trib1 in mice decreases lipid hepatic fat, while liver-specific...