A5000574162- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Cosmology and Gravitation Theories
- Dark Matter and Cosmic Phenomena
- Black Holes and Theoretical Physics
- Environmental DNA in Biodiversity Studies
- Genetic diversity and population structure
- Protein Kinase Regulation and GTPase Signaling
- RNA and protein synthesis mechanisms
- Particle physics theoretical and experimental studies
- Glycosylation and Glycoproteins Research
- Metabolism and Genetic Disorders
- Heat Transfer and Boiling Studies
- Forensic Anthropology and Bioarchaeology Studies
- Mitochondrial Function and Pathology
- Thyroid Disorders and Treatments
- Identification and Quantification in Food
- Cellular transport and secretion
- DNA Repair Mechanisms
- Protein purification and stability
- Erythrocyte Function and Pathophysiology
- Computational Physics and Python Applications
- Fluid Dynamics and Thin Films
- Genomics and Chromatin Dynamics
- Microfluidic and Capillary Electrophoresis Applications
The University of Tokyo
2003-2023
Shizuoka University
2013-2018
National Research Institute of Police Science
2003-2013
Hitachi (Japan)
2002
Teikyo University
1990-1992
University of Utah
1990
A genetic locus (D1S58, defined by DNA probe pMCT118) that contains a variable number of tandem repeats (VNTR) has been successfully amplified from very small amount genomic deoxyribonucleic acid (DNA) the polymerase chain reaction (PCR). The sequence was determined and found to consist 16-base consensus flanking sequences. Oligonucleotide primers complementary sequences were synthesized serve as for amplification MCT118 PCR method. Human isolated blood (2 ng each sample) at locus,...
The roles of selected amino acid residues human 14-kDa beta-galactoside-binding lectin were studied by site-directed mutagenesis. Ten mutant proteins produced, in each which one the regarded as possibly related to stability (6 cysteine residues) or those highly conserved vertebrate family (Asn46, Trp68, Glu71, and Arg73), was substituted. All lectins had been substituted with serine (C2S, C16S, C42S, C60S, C88S, C130S) proved have sugar binding ability comparable that wild-type lectin. In...
We have cloned a full-length cDNA for beta-galactoside-binding protein with relative molecular mass of 32 kDa (32-kDa GBP), recently purified from nematode, Caenorhabditis elegans (Hirabayashi, J., Satoh, M., Ohyama, Y., and Kasai, K. (1992) J. Biochem. 111, 553-555). The clone contained single open reading frame encoding 279 amino acids, including the initiator methionine. Significant sequence homology to metal-independent lectins (25-30% identities), which had previously been found only in...
The complete primary structure of chicken 16-kDa beta-galactoside-binding lectin (C-16) was determined. It composed 134 amino acid residues and has an acetylated NH2 terminus. A cDNA also cloned, but no signal sequence found in the initiator region. methionine remained as terminus mature lectin. Although C-16 is distinct from 14-kDa (C-14), it proved to be a member vertebrate 14-kDa-type family. Comparison structures between lectins suggests that C-14 were produced by gene duplication...
Abstract We study the clustering of primordial black holes (PBHs) and axion miniclusters produced in model proposed to explain LIGO/Virgo events or seeds supermassive (SMBHs) ref. [1]. It is found that this predicts large isocurvature perturbations due PBHs miniclusters, from which we obtain stringent constraints on parameters. Specifically, for decay constant f a = 10 16 GeV, potentially accounts SMBHs, PBH fraction dark matter should be ≲ 7 × -10 . Assuming mass increases by more than...
A quantitative PCR procedure using a newly designed primer set for amplification of human specific DNA sequence D17Z1 quantification has been developed. The was compared with the existing method, UV absorption method and slot blot hybridization method. lower limit quantification, reproducibility results, degraded DNA, specificity application forensic casework samples were examined each technique. consumed least amount could quantify as little 0.0015 ng its comparable STR typing quantified by...
Short tandem repeat studies are powerful tools for parentage analysis and identification of missing persons, victims murder, mass fatalities when reference samples unavailable. The primer in the Identifiler kit failed to amplify an allele at D19S433 locus, producing a silent ("null") allele. causal mutation is base change (G>A) 32 nucleotides downstream from 3' end AAGG repeats. alleles problematical because transmitted, they can cause parent-child inconsistency that unrelated Mendelian...
Abstract Sterile neutrino is a fascinating candidate for dark matter. In this paper, we examine the Affleck-Dine (AD) leptogenesis scenario generating large lepton asymmetry, which can induce resonant production of sterile matter via Shi-Fuller (SF) mechanism. We also revisit numerical calculation SF mechanism and constraints from current X-ray Lyman- α forest observations. find that AD explain by incorporating non-topological soliton with charge called L-ball. Finally, discuss an...
A denaturing gradient gel electrophoresis (DGGE) assay was used to detect mitochondrial DNA (mtDNA) sequence heteroplasmy in 160 hairs from each of three individuals. The HV1 and HV2 heteroplasmic positions were then identified by sequencing. In several hairs, the position not evident sequencing dHPLC separation homoduplex/heteroduplex species carried out with subsequent reamplification identify site. overall detection frequency these 5.8% (28/480) DGGE 4.4% (21/280) Sequence hair observed...
ABO types obtained from evidentiary samples have been used effectively to obtain the initial information leading apprehension of culprits in Japanese criminal investigations. A simple genotyping method using multiplex sequence-specific PCR and capillary electrophoresis was developed as a supplement serological typing. Limitations predicting phenotype based on genotype were evaluated 1134 randomly selected peripheral blood samples. concordance rate 99.82% (1132/1134 samples) found between...
Abstract We study a primordial black hole (PBH) formation scenario based on the Affleck-Dine (AD) mechanism and investigate two PBH mass regions: M ∼ 30 ⊙ motivated by LIGO-Virgo observations of binary mergers ≳ 10 4 supermassive holes at center galaxies. In previous studies, it has been considered that inhomogeneous AD baryogenesis generates regions with large baryon asymmetry, some which collapse into PBHs. this paper, we show is severely constrained due to asymmetry remaining outside...