A5052592817- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- CRISPR and Genetic Engineering
Eterna Massive Open Laboratory
2016-2023
Stanford University
2020
Stanford Medicine
2018
Designing RNAs that form specific secondary structures is enabling better understanding and control of living systems through RNA-guided silencing, genome editing protein organization. Little known, however, about which RNA might be tractable for downstream sequence design, increasing the time expense design efforts due to inefficient structure choices. Here, we present insights into structural features increase difficulty finding sequences fold a target structure, summarizing tens thousands...
Abstract Functional design of ribosomes with mutant ribosomal RNA (rRNA) can expand opportunities for understanding molecular translation, building cells from the bottom-up, and engineering altered capabilities. However, such efforts are hampered by cell viability constraints, an enormous combinatorial sequence space, limitations on large-scale, 3D structures functions. To address these challenges, we develop integrated community science experimental screening approach rational ribosomes....
Homopolymeric adenosine RNA plays numerous roles in both cells and noncellular genetic material. We report herein an unusual poly(A) signature chemical mapping data generated by the Eterna Massive Open Laboratory. Poly(A) sequences of length seven or more show unexpected results selective 2′-hydroxyl acylation read out primer extension (SHAPE) dimethyl sulfate (DMS) probing. This first appears grows to its maximum strength at ∼10. In a long sequence, substitution single A any other...
ABSTRACT Functional design of ribosomes with mutant ribosomal RNA (rRNA) could expand opportunities for understanding molecular translation, building cells from the bottom-up, and engineering altered capabilities. However, such efforts have been hampered by cell viability constraints, an enormous combinatorial sequence space, limitations on large-scale, 3D structures functions. To address these challenges, we developed integrated community science experimental screening approach rational...
ABSTRACT Homopolymeric adenosine RNA plays numerous roles in both cells and non-cellular genetic material, for lack of evidence to the contrary, it is generally accepted form a random coil under physiological conditions. However, chemical mapping data generated by Eterna Massive Open Laboratory indicates that poly (A) sequence length seven or more, at pH 8.0 MgCl concentrations 10 mM, develops unexpected protection selective 2’-hydroxyl acylation read out primer extension (SHAPE) dimethyl...