A5018767671- Parathyroid Disorders and Treatments
- Genetic Syndromes and Imprinting
- Ion channel regulation and function
- Fibroblast Growth Factor Research
- Bone health and osteoporosis research
- Bone health and treatments
- Erythrocyte Function and Pathophysiology
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Genetic and Kidney Cyst Diseases
- Magnesium in Health and Disease
- Myofascial pain diagnosis and treatment
- Musculoskeletal pain and rehabilitation
- Acupuncture Treatment Research Studies
- Heterotopic Ossification and Related Conditions
- Cellular Mechanics and Interactions
- Neuropeptides and Animal Physiology
- Bone and Joint Diseases
- Exercise and Physiological Responses
- Innovations in Medical Education
- Pain Mechanisms and Treatments
- Bone Metabolism and Diseases
- Health and Medical Research Impacts
- Muscle Physiology and Disorders
- bioluminescence and chemiluminescence research
- Advanced Biosensing Techniques and Applications
Indiana University – Purdue University Indianapolis
2011-2025
Indiana University School of Medicine
2010-2025
Marian University - Indiana
2016-2025
Indiana Biosciences Research Institute
2023-2025
University of Ottawa
2024
Columbia University
2024
John Wiley & Sons (United States)
2020
University of New England
2020
New England College
2020
Western University of Health Sciences
2020
Casimersen (AMONDYS 45TM) is an antisense oligonucleotide of the phosphorodiamidate morpholino oligomer subclass developed by Sarepta therapeutics. It was approved Food and Drug Administration (FDA) in February 2021 to treat Duchenne muscular dystrophy (DMD) patients whose DMD gene mutation amenable exon 45 skipping. Administered intravenously, casimersen binds pre-mRNA skip a mutated region exon, thereby producing internally truncated yet functional dystrophin protein patients. This...
α Klotho ( KL) regulates mineral metabolism, and diseases associated with KL deficiency are characterized by hyperphosphatemia vascular calcification (VC). is expressed as a membrane-bound protein (mKL) recognized the coreceptor for fibroblast growth factor-23 (FGF23) circulating soluble form (cKL) created endoproteolytic cleavage of mKL. The functions cKL regard to phosphate metabolism unclear. We tested ability regulate pathways phenotypes in mouse model CKD-mineral bone disorder -null...
ABSTRACT The transgenic and knockout (KO) animals involving Fgf23 have been highly informative in defining novel aspects of mineral metabolism, but are limited by shortened lifespan, inability spatial/temporal FGF23 control, infertility the global KO. To more finely test role systemic genetic influences production, a mouse was developed that carried floxed (“f”)-Fgf23 allele (exon 2 floxed) which demonstrated vivo recombination when bred to global-Cre mice (eIIa-cre). Mice homozygous for...
The phosphaturic hormone FGF23 is elevated in chronic kidney disease (CKD). risk of premature death substantially higher the CKD patient population, with cardiovascular (CVD) as leading mortality cause at all stages CKD. Elevated has been associated increased odds for all-cause mortality; however, whether positive adaptation unknown. To test role phenotypes, a late osteoblast/osteocyte conditional flox-Fgf23 mouse (Fgf23fl/fl/Dmp1-Cre+/–) was placed on an adenine-containing diet to induce...
Abstract Context Sarcopenia is a disease characterized by low muscle mass and function that places individuals at greater risk of disability, loss independence, death. Current therapies include addressing underlying performance issues, resistance training, and/or nutritional strategies. However, these approaches have significant limitations, chronic inflammation associated with sarcopenia may blunt the anabolic response to exercise nutrition. This presents an unmet need for treatment...
The acute effects of parathyroid hormone (PTH) on fibroblast growth factor 23 (FGF23) in vivo are not well understood. After a single subcutaneous PTH (1-34) injection (50 nmol/kg) mice, FGF23 levels were assessed plasma using assays that measure either intact alone (iFGF23) or intact/C-terminal (cFGF23). Furthermore, messenger RNA (mRNA) and protein bone. In addition, we examined the treatment production vitro differentiated calvarial osteocyte-like cells. cFGF23 increased by three- to...
Mechanotransduction, the process by which cells convert external mechanical stimuli such as fluid shear stress (FSS) into biochemical changes, plays a critical role in maintenance of skeleton. We have proposed that stimulation FSS across surfaces bone results formation unique signaling complexes called mechanosomes are launched from sites adhesion with extracellular matrix and other [1]. Deformation at cell membrane ultimately alteration target gene expression. Recently, we reported focal...
Mechanical stimulation of the skeleton promotes bone gain and suppresses loss, ultimately resulting in improved strength fracture resistance. The molecular mechanisms directing anabolic and/or anti-catabolic actions on during loading are not fully understood. Identifying mechanotransduction (MTD) signaling cascades could identify new therapeutic targets. Most research into MTD is typically focused understanding pathways that stimulate formation response to load. However, we investigated...
Fluid shear stress protects cells from TNF-α-induced apoptosis. Oscillatory fluid (OFSS) is generally perceived as physiologically relevant biophysical signal for bone cells. Here we identify several cellular mechanisms responsible mediating the protective effects of OFSS against apoptosis in vitro. We found that exposure MC3T3-E1 osteoblast-like to little 5 min suppressed activation caspase-3, cleavage PARP and phosphorylation histone. In contrast, H(2)O(2)-induced was not inhibited by...
Fibroblast growth factor-23 (FGF23) controls key responses to systemic phosphate increases through its phosphaturic actions on the kidney. In addition stimulation by phosphate, FGF23 positively responds iron deficiency anemia and hypoxia in rodent models humans. The disorder X-linked hypophosphatemia (XLH) is characterized elevated concert with an intrinsic bone mineralization defect. Indeed, Hyp mouse XLH model has disturbed osteoblast osteocyte differentiation altered expression of a wide...
Abstract Objective Soft tissue manual therapies are commonly utilized by osteopathic physicians, chiropractors, physical therapists and massage therapists. These techniques predicated on subjecting tissues to biophysical mechanical stimulation but the cellular molecular mechanism(s) mediating these effects poorly understood. Previous studies established an in vitro model system for examining of dermal fibroblasts that cyclical strain, intended mimic overuse injury, induces secretion numerous...
Abstract Osteocytes sense and respond to mechanical force by controlling the activity of other bone cells. However, mechanisms which osteocytes input transmit biological signals remain unclear. Voltage-sensitive calcium channels (VSCCs) regulate (Ca2+) influx in response external stimuli. Inhibition or deletion VSCCs impairs osteogenesis skeletal responses loading. VSCC is influenced its auxiliary subunits, bind channel’s α1 pore-forming subunit alter intracellular Ca2+ concentrations. The...
Abstract Diabetes results from insufficient insulin production by pancreatic islet β-cells or a loss of themselves. Restoration regulated is predominant goal translational diabetes research. Here, we provide brief overview recent advances in the fields β-cell proliferation, regeneration, and replacement. The discovery therapeutic targets associated small molecules has been enabled improved understanding development cell cycle regulation, as well advanced high-throughput screening...
Our understanding of how osteocytes, the principal mechanosensors within bone, sense and perceive force remains unclear. Previous work identified "tethering elements" (TEs) spanning pericellular space osteocytes transmitting mechanical information into biochemical signals. While we heparan sulfate proteoglycan perlecan (PLN) as a component these TEs, PLN must attach to cell surface induce responses. As voltage-sensitive calcium channels (VSCCs) are critical for bone mechanotransduction,...
Global loss of the neuropeptide Neuromedin-U (NMU) is associated with increased bone formation and high mass in male female mice by twelve weeks age, suggesting that NMU suppresses osteoblast differentiation and/or activity vivo. highly expressed numerous anatomical locations including skeleton hypothalamus. This raises possibility exerts indirect effects on remodeling from an extra-skeletal location such as brain. Thus, present study we used microinjection to deliver viruses carrying...
Diabetic nephropathy (DN) is a primary cause of end-stage renal disease and becoming more prevalent because the global rise in type 2 diabetes. A model DN, db/db uninephrectomized ( db/db-uni) mouse, characterized by obesity, as well compromised function. This also manifests defects mineral metabolism common including hyperphosphatemia, which leads to severe endocrine disease. The FGF23 coreceptor, α-Klotho, circulates soluble, cleaved form (cKL) may directly influence phosphate handling....
Low back pain (LBP) is a common musculoskeletal complaint that can impede physical function and mobility. Current management often involves medication, but there need for non-pharmacological non-invasive interventions. Soft tissue manipulation (STM) such as massage has been shown to be effective in human subjects, the molecular mechanisms underlying these findings are not well understood. In this paper, we evaluated potential changes soft levels of more than thirty pro- or anti-inflammatory...
There are limited data regarding the experiences of and attitudes toward research participation among osteopathic medical students despite rapidly increasing enrollment expansion number schools.To assess first-year students' experience with research, their interest in it, perceptions its value, barriers to participation.An anonymous, online survey was sent 868 class 2021 at 4 colleges medicine. The consisted 14 multiple-choice items (7 which offered option a written response) 1 open-ended...