A5041943864- Liver physiology and pathology
- Tissue Engineering and Regenerative Medicine
- Organ Transplantation Techniques and Outcomes
- Venous Thromboembolism Diagnosis and Management
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Clinical Nutrition and Gastroenterology
- Spacecraft and Cryogenic Technologies
- Adrenal Hormones and Disorders
- CAR-T cell therapy research
- Reproductive Biology and Fertility
- Inflammatory mediators and NSAID effects
- Advanced Thermodynamic Systems and Engines
- Lymphoma Diagnosis and Treatment
- Urological Disorders and Treatments
- Cardiac tumors and thrombi
- Glycosylation and Glycoproteins Research
- Pluripotent Stem Cells Research
- Blood disorders and treatments
- Chronic Lymphocytic Leukemia Research
The Royal Free Hospital
2016-2017
University College London
2014-2017
Asymptote (United Kingdom)
2013-2014
Cryopreservation protocols are increasingly required in regenerative medicine applications but must deliver functional products at clinical scale and comply with Good Manufacturing Process (GMP). While GMP cryopreservation is achievable on a small using Stirling cryocooler-based controlled rate freezer (CRF) (EF600), successful large-scale more challenging due to heat transfer issues control of ice nucleation, both complex events that impact success. We have developed CRF (VIA Freeze) can...
Liver failure, whether arising directly from acute liver failure or decompensated chronic disease is an increasing problem worldwide and results in many deaths. In the UK only 10% of individuals requiring a transplant receive one. Thus need for alternative treatments paramount. A BioArtificial machine could temporarily replace functions liver, buying time patient's to repair regenerate. We have designed, implemented tested clinical-scale containing biomass derived hepatoblastoma cell-line...
There have been relatively few studies on the implications of physical conditions experienced by cells during large volume (litres) cryopreservation - most focused problem smaller volumes, typically up to 2 ml. This study explores effects ice growth progressive solidification, generally seen larger scale cryopreservation, encapsulated liver hepatocyte spheroids, and it develops a method reliably sample different regions across frozen cores samples experiencing solidification. These issues...
For large and complex tissue engineered constructs to be available on demand, long term storage using methods, such as cryopreservation, are essential. This study optimised parameters excess media concentration warming rates used the findings enable successful cryopreservation of 2.3 litres alginate encapsulated liver cell spheroids. volume biomass is typical those required for treatment Acute Liver Failure our Bioartificial Device. Adding a buffer medium above biomass, well slow (0.6°C/min)...
For many bioengineered tissues to have practical clinical application, cryopreservation for use on demand is essential. This study examined different thermal histories warming and short holding periods at subzero temperatures subsequent functional recoveries of alginate encapsulated liver spheroids (ELS) in a bioartificial device. mimicked transport liquid nitrogen (-196°C) or dry ice (∼-80°C) temperatures. Holding -80°C after -196°C storage resulted ELS expressing significant (p < 0.001)...