A5047512990- Uterine Myomas and Treatments
- Sarcoma Diagnosis and Treatment
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Endometrial and Cervical Cancer Treatments
- Tissue Engineering and Regenerative Medicine
- Liver physiology and pathology
- Cancer Genomics and Diagnostics
- Endometriosis Research and Treatment
- Reproductive Biology and Fertility
- Polymer Surface Interaction Studies
- TGF-β signaling in diseases
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Electrohydrodynamics and Fluid Dynamics
- Organ Transplantation Techniques and Outcomes
- Infrared Thermography in Medicine
- Erythrocyte Function and Pathophysiology
- Reproductive System and Pregnancy
- Renal cell carcinoma treatment
- Clinical Nutrition and Gastroenterology
Karolinska Institutet
2019-2024
Queen Mary University of London
2023-2024
Imperial College London
2023
Svenska Örtmedicinska Institute
2022
Karolinska University Hospital
2019
The Royal Free Hospital
2016-2019
University College London
2012-2019
Abstract Due to its dynamic nature, the evolution of cancer cell-extracellular matrix (ECM) crosstalk, critically affecting metastasis and treatment resistance, remains elusive. Our results show that platinum-chemotherapy itself enhances resistance by progressively changing cell-intrinsic adhesion signaling cell-surrounding ECM. Examining ovarian high-grade serous carcinoma (HGSC) transcriptome histology, we describe fibrotic ECM heterogeneity at primary tumors distinct metastatic sites,...
Abstract Stem cells are known to sense and respond the mechanical properties of biomaterials. In turn, exert forces on their environment that can lead striking changes in shape, size contraction associated tissues, may result disruption functional failure. However, no study has so far correlated stem cell phenotype biomaterials toughness. Indeed, disentangling toughness‐mediated response from other mechanosensing processes remained elusive as it is particularly challenging uncouple Youngs'...
Liver failure, whether arising directly from acute liver failure or decompensated chronic disease is an increasing problem worldwide and results in many deaths. In the UK only 10% of individuals requiring a transplant receive one. Thus need for alternative treatments paramount. A BioArtificial machine could temporarily replace functions liver, buying time patient's to repair regenerate. We have designed, implemented tested clinical-scale containing biomass derived hepatoblastoma cell-line...
Fibrillar collagens promote cell proliferation, migration, and survival in various epithelial cancers are generally associated with tumor aggressiveness. However, the impact of fibrillar on soft tissue sarcoma behavior remains poorly understood. Unexpectedly, this study finds that collagen-related gene expression is favorable patient prognosis rhabdomyosarcoma. By developing using collagen matrices distinct stiffness vivo-like microarchitectures, uncovers activation DDR1 has pro-apoptotic...
Delivery of cell therapies often requires the ability to hold products in readiness whilst logistical, regulatory and potency considerations are dealt with recorded.This reversibly stopping biological time, a process which is achieved by cryopreservation.However, cryopreservation itself poses many biophysical challenges living cells that need be understood order apply low temperature technologies their best advantage.This review sets out history applied cryopreservation, our current...
Abstract Purpose: Undifferentiated uterine sarcomas (UUS) are rare, extremely deadly, with no effective treatment. The goal of this study was to identify novel intrinsic molecular UUS subtypes using integrated clinical, histopathologic, and evaluation a large, fully annotated, patient cohort. Experimental Design: Fifty cases full clinicopathologic annotation were analyzed for gene expression (n = 50), copy-number variation (CNV, n 40), cell morphometry 39), protein 22). Gene ontology network...
Uterine sarcomas are rare but deadly malignancies without effective treatment. Immunotherapy is a promising new approach to treat these tumors has shown heterogeneous effects in sarcoma patients. With the goal of identifying key factors for improved patient treatment, we characterized tumor immune landscape 58 uterine cases with full clinicopathological annotation. Immune cell characterization revealed overall prevalence FOXP3+ cells and pro-tumor M2-like macrophages. Hierarchical clustering...
Abstract Soluble macromolecules present in the tumour microenvironment (TME) alter physical characteristics of extracellular fluid and can affect cancer cell behaviour. A fundamental step progression is formation a new vascular network which may originate from both pre-existing normal endothelium cancer-derived cells. To study role TME affecting endothelial cells we exposed to inert polymer solutions with different physicochemical characteristics. The line SK-HEP-1, but not human umbilical...
Delivery of cells into tubular tissue constructs with large diameters poses significant spatial and temporal challenges. This study describes preliminary findings for a novel process rapid uniform seeding onto the luminal surface constructs. Fibroblasts, tagged superparamagnetic iron oxide nanoparticles (SPION), were directed by magnetic field generated k4-type Halbach cylinder device. The distribution attached cells, as measured mean number was compared conventional, dynamic, rotational...
For many bioengineered tissues to have practical clinical application, cryopreservation for use on demand is essential. This study examined different thermal histories warming and short holding periods at subzero temperatures subsequent functional recoveries of alginate encapsulated liver spheroids (ELS) in a bioartificial device. mimicked transport liquid nitrogen (-196°C) or dry ice (∼-80°C) temperatures. Holding -80°C after -196°C storage resulted ELS expressing significant (p < 0.001)...
Fibrillar collagen deposition, stiffness and downstream signalling support the development of leiomyomas (LMs), common benign mesenchymal tumours uterus, are associated with aggressiveness in multiple carcinomas. Compared epithelial carcinomas, however, impact fibrillar collagens on malignant tumours, including uterine leiomyosarcoma (uLMS), remains elusive. In this study, we analyse network morphology density combined gene expression within uLMS, LM normal myometrium (MM). We find that,...
<title>Abstract</title> Uterine leiomyosarcoma (uLMS) is a rare and aggressive cancer representing approximately 2-5% of all uterine malignancies. The molecular heterogeneity pathogenesis uLMS are not well understood translational studies with the aim discovering vulnerabilities this tumor type high priority. We conducted an innovative comprehensive multi-omics integration study from DNA to protein using fresh frozen tumors unravel unprecedented features uLMS. Here we present that two harbor...
Abstract High-grade serous ovarian cancer, the most common and aggressive form of generally metastasises to visceral adipose tissues. In these tissues, extracellular matrix through which cancer cells adhere migrate is confined by presence preponderance adipocytes. How in this unique environment not known, yet critical understanding metastatic progression. To study processes, we develop biomimetic organo-hydrogels that recreate structural mechanical properties human We show present invasive...
Event Abstract Back to Use of non-adherent hydrogels control the behaviour human hepatocarcinoma cell lines Jordi Gonzalez-Molina1*, Natasha Maurmann1* and Clare Selden1* 1 University College London, Institute for Liver Digestive Health, United Kingdom Introduction: Cells are able sense physical chemical properties their surroundings mainly via proteins located in plasma membrane. Integrins transmembrane receptors that bind components extracellular matrix sending information interior cell....
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