A5038379862- Prostate Cancer Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Estrogen and related hormone effects
- Cancer Genomics and Diagnostics
- Urologic and reproductive health conditions
- ATP Synthase and ATPases Research
- Hormonal Regulation and Hypertension
- Hypothalamic control of reproductive hormones
- Gut microbiota and health
- Sexual Differentiation and Disorders
- Cancer, Hypoxia, and Metabolism
- Growth Hormone and Insulin-like Growth Factors
- Trace Elements in Health
- Cell Adhesion Molecules Research
- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Radiomics and Machine Learning in Medical Imaging
- Molecular Biology Techniques and Applications
- Bladder and Urothelial Cancer Treatments
- Redox biology and oxidative stress
- Urinary Tract Infections Management
University of Turku
2010-2024
Turku University Hospital
2013-2024
University of Foggia
2021
Weatherford College
1997
Abstract Due to its dynamic nature, the evolution of cancer cell-extracellular matrix (ECM) crosstalk, critically affecting metastasis and treatment resistance, remains elusive. Our results show that platinum-chemotherapy itself enhances resistance by progressively changing cell-intrinsic adhesion signaling cell-surrounding ECM. Examining ovarian high-grade serous carcinoma (HGSC) transcriptome histology, we describe fibrotic ECM heterogeneity at primary tumors distinct metastatic sites,...
Chemotherapy resistance is a critical contributor to cancer mortality and thus an urgent unmet challenge in oncology. To characterize chemotherapy processes high-grade serous ovarian cancer, we prospectively collected tissue samples before after analyzed their transcriptomic profiles at single-cell resolution. After removing patient-specific signals by novel analysis approach, PRIMUS, found consistent increase stress-associated cell state during chemotherapy, which was validated RNA situ...
The broad research use of organoids from high-grade serous ovarian cancer (HGSC) has been hampered by low culture success rates and limited availability fresh tumor material. Here, we describe a method for generation long-term expansion HGSC with efficacy markedly improved over previous reports (53% vs. 23%-38%). We established cryopreserved material, demonstrating the feasibility using viably biobanked tissue organoid derivation. Genomic, histologic, single-cell transcriptomic analyses...
Previous studies suggest that Leber9s hereditary optic neuropathy (LHON) may be a systemic disorder with manifestations in organs other than the nerves. To evaluate nervous system involvement 38 men and eight women LHON were re-examined. The patients divided into three groups according to mtDNA analysis--namely, 11778 or 3460 mutation without these primary mutations. Fifty nine per cent of had neurological abnormalities but there was no significant difference between groups. Movement...
Magnetic resonance imaging (MRI) combined with targeted biopsy (TB) is increasingly used in men clinically suspected prostate cancer (PCa), but the long acquisition times, high costs, and inter-center/reader variability of routine multiparametric MRI limit its wider adoption.The aim was to validate a previously developed unique reporting protocol, IMPROD biparametric (bpMRI) (NCT01864135), clinical suspicion PCa multi-institutional trial (NCT02241122). bpMRI has average time 15 minutes (no...
Although prostate cancer (PCa) is the most common in men Western countries, there significant variability geographical incidence. This might result from genetic factors, discrepancies screening policies, or differences lifestyle. Gut microbiota has recently been associated with progression, but its role PCa unclear. Characterization of gut and functions PCa. In a prospective multicenter clinical trial (NCT02241122), profiles 181 suspicion were assessed utilizing 16S rRNA sequencing....
Prostate cancer (PCa) and benign prostatic hyperplasia (BPH) are prevalent conditions affecting a significant portion of the male population, particularly with advancing age. Traditional diagnostic methods, such as digital rectal examination (DRE) prostate-specific antigen (PSA) tests, have limitations in specificity sensitivity, leading to potential overdiagnosis unnecessary biopsies.
Many patients with muscle-invasive bladder cancer (BC) are either ineligible for or do not benefit from cisplatin-based chemotherapy, and there is an unmet need to estimate individuals' drug sensitivities. We investigated the suitability of conditionally reprogrammed (CR) cells characterization BC properties their feasibility personalized sensitivity screening. The CR cultures were established six tumors varying histology stage. Four successfully propagated genomic, transcriptomic, protein...
Hydroxysteroid (17β) dehydrogenase 7 (HSD17B7) has been shown to catalyze the conversion of both estrone estradiol (17-ketosteroid reductase activity) and zymosterone zymosterol (3-ketosteroid activity involved in cholesterol biosynthesis) vitro. To define metabolic role enzyme vivo, we generated knockout mice deficient (HSD17B7KO). The data showed that lack HSD17B7 results a blockage de novo biosynthesis mouse embryos HSD17BKO die at embryonic day (E) 10.5. Analysis neural structures...
Background Multiparametric MRI of the prostate has been shown to improve risk stratification men with an elevated prostate‐specific antigen (PSA). However, long acquisition time, high cost, and inter‐center/reader variability a routine multiparametric limit its wider adoption. Purpose To develop validate nomograms based on unique rapid biparametric (bpMRI) qualitative quantitative derived variables for prediction clinically significant cancer (SPCa). Study Type Retrospective analyses single...
A major challenge in analyzing cancer patient transcriptomes is that the tumors are inherently heterogeneous and evolving. We analyzed 214 bulk RNA samples of a longitudinal, prospective ovarian cohort found sample composition changes systematically due to chemotherapy between anatomical sites, preventing direct comparison treatment-naive treated samples.To overcome this, we developed PRISM, latent statistical framework simultaneously extract cell-type-specific whole-transcriptome profiles...
Considerable attention has been paid to the role of sex steroids during periods major skeletal turnover, but interaction gonadotropic hormones, which include LH, FSH, and human chorionic gonadotropin (hCG), within bone tissue have overlooked. The question is pertinent due recent detection extragonadal expression receptors. Western blotting, immunolocalization, RT-PCR supported presence osteoblast LH However, cells failed bind [(125)I]hCG treatment with hCG generate either cAMP or...
FSH is a pituitary gonadotropin that along with LH plays key role in the regulation of gonadal function. The gonadotropic hormones are composed two subunits, common α subunit and hormone-specific β subunit, which determines binding to specific receptors induction biological response. Unlike LHβ gene, shown earlier studies harbour several amino acid-altering polymorphisms mutations, information about eventual sequence variation human FSHβ not available. In this study, we made analysis...
Hydroxysteroid (17beta) dehydrogenases (HSD17Bs) belong to the short-chain dehydrogenase/reductase family consisting of a diverse pool enzymes with oxidoreductase activity. HSD17B catalyze conversion between 17-keto and 17-hydroxy steroids, either activating or inactivating sex steroids. Previous studies have demonstrated role for human HSD17B1 enzyme in estradiol (E2) biosynthesis both gonads extragonadal steroid target tissues various estrogen-dependent diseases. In present study, five...
The functional properties of actin-regulating formin proteins are diverse and in many cases cell-type specific. FHOD1, a expressed predominantly cells mesenchymal lineage, bundles actin filaments participates maintenance cell shape, migration cellular protrusions. FHOD1 cancer-associated epithelial to transition (EMT) oral squamous carcinoma breast cancer. role melanomas has not been characterized. Here, we show that expression is typically strong cutaneous cultured melanoma while the low or...
Abstract Urinary extracellular vesicles (uEVs) are enriched with glycosylated proteins which have been extensively studied as putative biomarkers of urological cancers. Here, we characterized the glycosylation and integrin profile EVs derived from cancer cell lines. We used fluorescent europium‐doped nanoparticles coated lectins antibodies to identify a biomarker combination consisting subunit alpha 3 (ITGA3) fucose. In addition, same line‐derived analytical standards assess sensitivity...