A5067156052- Cancer Cells and Metastasis
- Autophagy in Disease and Therapy
- Ovarian cancer diagnosis and treatment
- Glioma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Reproductive Biology and Fertility
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Cancer Genomics and Diagnostics
- Cellular Mechanics and Interactions
- Cancer-related molecular mechanisms research
University of Copenhagen
2016-2023
The broad research use of organoids from high-grade serous ovarian cancer (HGSC) has been hampered by low culture success rates and limited availability fresh tumor material. Here, we describe a method for generation long-term expansion HGSC with efficacy markedly improved over previous reports (53% vs. 23%-38%). We established cryopreserved material, demonstrating the feasibility using viably biobanked tissue organoid derivation. Genomic, histologic, single-cell transcriptomic analyses...
Hypoxia-inducible factors enhance glioma stemness, and stem cells have an amplified hypoxic response despite residing within a perivascular niche. Still, little is known about differential HIF regulation in versus bulk cells. We show that the intracellular domain of cell marker CD44 (CD44ICD) released at hypoxia, binds HIF-2α (but not HIF-1α), enhances target gene activation, required for hypoxia-induced stemness glioma. In mouse model, was restricted to tumor regions, human glioma, hypoxia...
During the metastatic process, breast cancer cells must come into contact with extra-cellular matrix (ECM) at every step. The ECM provides both structural support and biochemical cues, cell–ECM interactions can lead to changes in drug response. Here, we used fibroblast-derived (FDM) perform high throughput screening of 4T1 on organ (lung), see that response differs from treatment plastic. FDMs produce different organs are abundant contains a complex mixture proteins. We also show differences...
Abstract The broad research use of organoids from high-grade serous ovarian carcinoma (HGSC) has been hampered by low culture success rates and limited availability fresh tumor material. Here we describe a method for generation long-term expansion HGSC with efficacy markedly improved over previous reports (55% vs. 23-38%). We established cryopreserved material, demonstrating the feasibility using viably biobanked tissue organoid derivation. Genomic, histologic single-cell transcriptomic...
Abstract Extensive utilization of organoids from high-grade serous ovarian carcinoma (HGSOC), the most common and lethal cancer, has been hampered by low success rates long-term culture scarcity fresh tumor material. Here we present development a novel method for efficient generation, expansion use HGSOC cryopreserved First, assessed commonly used organoid media components found that supplements such as FGF-2, R-Spondin1, Wnt or Noggin had negative impact on derivation. But further,...