A5027567583- Autophagy in Disease and Therapy
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Endoplasmic Reticulum Stress and Disease
- PARP inhibition in cancer therapy
- Cancer-related gene regulation
- Toxoplasma gondii Research Studies
- Polyamine Metabolism and Applications
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Telomeres, Telomerase, and Senescence
- Advanced biosensing and bioanalysis techniques
- Calcium signaling and nucleotide metabolism
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Advanced Proteomics Techniques and Applications
- RNA regulation and disease
- Plant responses to water stress
- Genomics and Chromatin Dynamics
- Cellular transport and secretion
- Mass Spectrometry Techniques and Applications
University of Copenhagen
2014-2025
Danish Cancer Society
2006-2025
Institute for Medical Research
2007
MicroRNAs are emerging as important regulators of cancer-related processes. The miR-21 microRNA is overexpressed in a wide variety cancers and has been causally linked to cellular proliferation, apoptosis, migration. Inhibition mir-21 MCF-7 breast cancer cells causes reduced cell growth. Using array expression analysis depleted miR-21, we have identified mRNA targets shown link between the p53 tumor suppressor protein. We furthermore found that protein Programmed Cell Death 4 (PDCD4)...
Background MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized key players in tumorigenesis. miR-145 is reported to be down-regulated several cancers, but knowledge of its targets colon cancer remains limited. Methodology/Principal Findings To investigate the role cancer, we employed a microarray based approach identify targets. Based on seed site enrichment analyses unbiased word analyses, found significant miRNA binding sites 3′-untranslated regions (UTRs)...
Abstract Background MicroRNAs (miRNAs) are well recognized as gene regulators and have been implicated in the regulation of development human diseases. miR-143 is located at a fragile site on chromosome 5 frequently deleted cancer, has reported to be down-regulated several cancers including colon cancer. Methods To gain insight into role we used microarray-based approach combination with seed enrichment analysis identify targets. Results As expected, transcripts upon overexpression had...
Abstract Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting coordinated response to stimuli. Mass spectrometry-based proteomics combination with fractionation can provide comprehensive maps spatio-temporal regulation protein networks cells, but involves laborious workflows does not cover the phospho-proteome level. Here we present high-throughput workflow based on sequential cell profile global proteome and...
Plasma membrane regeneration after injury requires the removal of damaged via LC3-associated macropinocytosis.
Impairment of translation can lead to collisions ribosomes, which constitute an activation platform for several ribosomal stress-surveillance pathways. Among these is the ribotoxic stress response (RSR), where sensing by MAP3K ZAKα leads p38 and JNK kinases. Despite insights, physiological ramifications impairment downstream RSR signaling remain elusive. Here, we show that stalling ribosomes sufficient activate ZAKα. In amino acid deprivation full nutrient starvation, impacts on ensuing...
The centrosome is the master orchestrator of mitotic spindle formation and chromosome segregation in animal cells. Centrosome abnormalities are frequently observed cancer, but little known their origin about pathways affecting homeostasis. Here we show that autophagy preserves organization stability through selective turnover centriolar satellite components, a process termed doryphagy. Autophagy targets organizer PCM1 by interacting with GABARAPs via C-terminal LIR motif. Accordingly,...
Autophagy is an evolutionarily conserved process that captures aberrant intracellular proteins and/or damaged organelles for delivery to lysosomes, with implications cellular and organismal homeostasis, aging diverse pathologies, including cancer. During cancer development, autophagy may play both tumour-supporting tumour-suppressing roles. Any relationships of the established oncogene-induced replication stress (RS) ensuing DNA damage response (DDR)-mediated anti-cancer barrier in early...
Oncogene-induced senescence (OIS) is a persistent anti-proliferative response that acts as barrier against malignant transformation. During OIS, cells undergo dynamic remodeling, which involves alterations in protein and organelle homeostasis through autophagy. Here, we show ribosomes are selectively targeted for degradation by autophagy during OIS. By characterizing senescence-dependent the ribosomal interactome, find deubiquitinase USP10 dissociates from ribosome transition to This release...
ABSTRACT RNA interference (RNAi) is now widely used for gene silencing in mammalian cells. The mechanism uses the RNA-induced complex, which Dicer, Ago2, and human immunodeficiency virus type 1 (HIV-1) TAR binding protein (TRBP) are main components. TRBP a that increases HIV-1 expression replication by inhibition of interferon-induced kinase PKR increasing translation viral mRNA. After HIV infection, could restrict through its activity RNAi or contribute more to enhancement replication. To...
Nutrient deprivation is a major trigger of autophagy, conserved quality control and recycling process essential for cellular tissue homeostasis. In high-content image-based screen the human ubiquitome, we here identify E3 ligase Pellino 3 (PELI3) as crucial regulator starvation-induced autophagy. Mechanistically, PELI3 localizes to autophagic membranes, where it interacts with ATG8 proteins through an LC3-interacting region (LIR). This facilitates PELI3-mediated ubiquitination ULK1, driving...
Cancer cells utilize lysosomes for invasion and metastasis. Myeloid Zinc Finger1 (MZF1) is an ErbB2-responsive transcription factor that promotes of breast cancer via upregulation lysosomal cathepsins B L. Here we identify let-7 microRNA, a well-known tumor suppressor in cancer, as direct negative regulator MZF1. Analysis primary tissues reveals gradual MZF1 from normal epithelium to invasive ductal carcinoma correlation between several family members mRNA, suggesting the inverse regulatory...
EIF4A3 (eukaryotic translation initiation factor 4A3) is an RNA helicase and core component of the exon junction complex. While this RNA-binding protein (RBP) well-characterized for its crucial roles in splicing, trafficking nonsense-mediated decay, role regulation metabolic signaling pathways remains elusive. In a recent study, we describe new as negative regulator macroautophagy/autophagy. Mechanistically, report that EIF4A3, through ability to safeguard can maintain low basal levels...