A5102725532- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Neuroendocrine Tumor Research Advances
- Epigenetics and DNA Methylation
- Thyroid Cancer Diagnosis and Treatment
- Glioma Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Sarcoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Renal cell carcinoma treatment
- Genomic variations and chromosomal abnormalities
- Pancreatitis Pathology and Treatment
- Thyroid Disorders and Treatments
- Cancer Mechanisms and Therapy
- Oral and Maxillofacial Pathology
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Pulmonary Hypertension Research and Treatments
- Liver Disease Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Colorectal and Anal Carcinomas
- Cardiovascular and Diving-Related Complications
University of Milan
1998-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2024
Vita-Salute San Raffaele University
2024
IRCCS Ospedale San Raffaele
2024
Ospedale per gli Infermi
2020-2023
Azienda Unità Sanitaria Locale Della Romagna
2021
Policlinico S.Orsola-Malpighi
2012-2020
University of Bologna
2013-2020
Ospedale degli Infermi
2020
Hypoxia-inducible factors enhance glioma stemness, and stem cells have an amplified hypoxic response despite residing within a perivascular niche. Still, little is known about differential HIF regulation in versus bulk cells. We show that the intracellular domain of cell marker CD44 (CD44ICD) released at hypoxia, binds HIF-2α (but not HIF-1α), enhances target gene activation, required for hypoxia-induced stemness glioma. In mouse model, was restricted to tumor regions, human glioma, hypoxia...
Autonomous thyroid adenomas (ATAs) are a frequent cause of hyperthyroidism. Mutations in the genes encoding TSH receptor (TSHR) or Gs protein α subunit (GNAS) found approximately 70% ATAs. The involvement other and pathogenesis remaining cases presently unknown. Here, we performed whole-exome sequencing 19 ATAs that were paired with normal DNA samples identified recurrent hot-spot mutation (c.1712A>G; p.Gln571Arg) enhancer zeste homolog 1 (EZH1) gene, which codes for catalytic polycomb...
Abstract Background Immune checkpoint inhibitors (ICIs) reported remarkable achievements in several solid tumours. However, metastatic colorectal cancer (mCRC) promising results are limited to patients with deficient mismatch repair/microsatellite instability-high (dMMR/MSI-high) tumours due their immune-enriched microenvironment. Combining cytotoxic agents and bevacizumab mCRC proficient stability (pMMR/MSS) could make ICIs efficacious by increasing the exposure of neoantigens, especially...
This study investigated the efficacy of chemoradiotherapy (CRT) followed by durvalumab as neoadjuvant therapy locally advanced rectal cancer.The PANDORA trial is a prospective, phase II, open-label, single-arm, multicenter aimed at evaluating and safety preoperative treatment with (1500 mg every 4 weeks for three administrations) following long-course radiotherapy (RT) plus concomitant capecitabine (5040 cGy RT in 25-28 fractions over 5 administered 825 mg/m2 twice daily). The primary...
Aim: The purpose of the present multicentric study was to review stereotactic body radiotherapy (SBRT) with or without chemotherapy (CHT) experience in locally advanced pancreatic cancer (LAPC). Endpoints were overall survival (OS), local control (LC), and distant metastasis-free (DMFS). Several parameters9 impact on these outcomes assessed. Materials Methods: Fifty-six patients LAPC undergoing SBRT+/-CHT included. SBRT median BED<sub>α/β10Gy</sub> 48.0 Gy (range=28.0-78.7). Survival curves...
// Elisa Stellaria Grassi 1 , Valeria Vezzoli Irene Negri 2,7 Árpád Lábadi 3 Laura Fugazzola 4,5 Giovanni Vitale 1,2,6 and Luca Persani DISCCO, Department of Clinical Sciences Community Health, University Milan, Italy 2 Laboratory Endocrine Metabolic Research, Istituto Auxologico Italiano IRCCS, Cusano Milanino, Medicine, Pécs, Hungary 4 Pathophysiology Transplantation, 5 Unit-Fondazione IRCCS Ca' Granda, 6 Division Diseases, 7 Current address: IRIBHM, Institute Interdisciplinary Research in...
Abstract Glioblastoma multiforme is characterized in part by severe hypoxia associated with tumor necrosis. The cellular response to can influence several properties of cells aggressive growth, including metabolic adaptations and cell migration invasion. Here, we found that Delta Like Non-Canonical Notch Ligand 1 (DLK1) expression was elevated as compared normal brain a genetically engineered mouse model glioma, DLK1 increased grade human glioma samples. highest hypoxic perivascular areas,...
Cyclic AMP (cAMP) inhibits the proliferation of several tumor cells. We previously reported an antiproliferative effect PKA I-selective cAMP analogs (8-PIP-cAMP and 8-HA-cAMP) on two human cancer cell lines different origin. 8-Cl-cAMP, another analog with known properties, has been investigated as a potential anticancer drug. Here, we compared 8-Cl-cAMP in three (ARO, NPA WRO). had similarly potent effects BRAF-positive ARO cells, but not BRAF-negative WRO which only consistently inhibited...
Medullary thyroid cancer (MTC) is a tumor highly resistant to chemo- and radiotherapy. Drug resistance can be induced by epigenetic changes such as aberrant DNA methylation. To overcome drug resistance, we explored promising approach based on the use of 5-aza-2'-deoxycytidine (AZA), demethylating agent, in combination with mTOR inhibitor everolimus MTC cells (MZ-CRC-1 TT). This combined treatment showed strong synergistic antiproliferative activity through induction apoptosis. The effect...
The aim of the current study was to implement whole transcriptome massively parallel sequencing (RNASeq) and copy number analysis investigate molecular biology pancreatic ductal adenocarcinoma (PDAC). Samples from 16 patients with PDAC were collected by ultrasound‑guided biopsy or surgical specimens for DNA RNA extraction. All samples analyzed RNASeq performed at 75x2 base pairs on a HiScanSQ Illumina platform. Single‑nucleotide variants (SNVs) detected SNVMix filtered dbSNP, 1000 Genomes...
Context:Congenital hypothyroidism (CH) is one of the most common inborn endocrine disorders with genetic background. Despite well-established newborn CH screening program in Hungary, no systematic examination underlying alterations has been performed as yet.
Multicellular spheroids represent an interesting experimental model with promising applications in the pre-clinical studies on anticancer drugs. We recently demonstrated that thyroid recapitulate features of original tissues, either differentiated and "stem-like" components. Here we were aimed to characterize investigate vivo proangiogenic potential patient-derived xenografts (PDX) obtained from papillary cancer (PTC) matched normal tissues.Spheroids cultures 11 PTCs tissues characterized by...
// Valentina Cirello 1, 2 , Vaira 3 Elisa Stellaria Grassi 4 Valeria Vezzoli Dario Ricca Carla Colombo 5 Silvano Bosari 2, Leonardo Vicentini 6 Luca Persani 4, Stefano Ferrero 3, 7 Laura Fugazzola 1 Endocrine Unit, Fondazione IRCCS Ca' Granda, 20122 Milan Department of Pathophysiology and Transplantation, University Milan, Division Pathology, Laboratory Metabolic Research, Istituto Auxologico Italiano IRCCS, 20149 Clinical Sciences Community Health, Surgery Biomedical, Surgical Dental...
Tumor cell behaviors associated with aggressive tumor growth such as proliferation, therapeutic resistance, and stem characteristics are regulated in part by soluble factors derived from the microenvironment. Tumor-associated astrocytes represent a major component of glioma microenvironment, have an active role maintenance normal neural cells niche, via secretion delta-like noncanonical Notch ligand 1 (DLK1). We found that astrocytes, when exposed to stresses microenvironment hypoxia or...
Despite the genomic characterization of pancreatic cancer (PC), marked advances in development prognosis classification and novel therapeutic strategies have yet to come. The present study aimed better understand alterations associated with invasive phenotype PC, order improve patient selection for treatment options. A total 30 PC samples were analysed by either whole transcriptome (9 samples) or exome sequencing (21 on an Illumina platform (75X2 100X2 bp), results matched normal DNA...
Introduction FOXE1 is required for thyroid function and its homozygous mutations cause a rare syndromic form of congenital hypothyroidism (CH). has polymorphic polyalanine tract whose involvement in pathology controversial. Starting from genetic studies CH family, we explored the functional role variations large population. Methods We applied NGS screening to family cohort 1752 individuals validated these results by silico modeling vitro experiments. Results A new heterozygous variant...