A5061814902- Gut microbiota and health
- Diet and metabolism studies
- Dermatology and Skin Diseases
- Bone Tissue Engineering Materials
- Clostridium difficile and Clostridium perfringens research
- Dietary Effects on Health
- Dental materials and restorations
- Infant Health and Development
- Liver Disease Diagnosis and Treatment
- Urinary Tract Infections Management
- Breastfeeding Practices and Influences
- Medicine and Dermatology Studies History
- Microscopic Colitis
- Muscle metabolism and nutrition
- Tryptophan and brain disorders
- Probiotics and Fermented Foods
- Metabolomics and Mass Spectrometry Studies
- Appendicitis Diagnosis and Management
- Facial Trauma and Fracture Management
- Bone health and osteoporosis research
- Intraperitoneal and Appendiceal Malignancies
- Adipokines, Inflammation, and Metabolic Diseases
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Maternal Mental Health During Pregnancy and Postpartum
University of Turku
2016-2025
Turku University Hospital
2016-2025
Turku Centre for Biotechnology
2022
University of Jyväskylä
2010-2016
Abstract Dissolution behavior of six bioactive glasses was correlated with the antibacterial effects same against sixteen clinically important bacterial species. Powdered (<45 μm) were immersed in simulated body fluid (SBF) for 48 h. The pH solution inside glass powder measured situ a microelectrode. After 2, 4, 27, and h, concentration ions after removing particles mixing SBF normal electrode ICP‐OES. bacteria cultured broth up to 4 days, which viability determined. effect increased...
Recent studies suggest that exercise alters the gut microbiome. We determined whether six-weeks endurance exercise, without changing diet, affected metagenome and systemic metabolites of overweight women. Previously sedentary women (n = 19) underwent a intervention, but two were excluded due to antibiotic therapy. The microbiota composition functions analyzed by 16S rRNA gene amplicon sequencing metagenomics. Body was with DXA X-ray densitometer serum metabolomics NMR metabolomics. Total...
Next-generation sequencing (NGS) is currently the method of choice for analyzing gut microbiota composition. As composition a potential future target clinical diagnostics, it utmost importance to enhance and optimize NGS analysis procedures. Here, we have analyzed impact DNA extraction selected 16S rDNA primers on results. Bacterial from frozen stool specimens was extracted with 5 commercially available kits. Special attention paid semiautomated methods that could expedite procedure, thus...
One of the key behavioral phenotypes in infancy are different temperament traits, and certain early life traits have been shown to precede later mental health problems. Differences gut microbiota composition (GMC) suggested link with neurodevelopment. For example, toddler found associate differences GMC; however, studies infants lacking although is a rapid period neurodevelopment as well GM development. Thus, we aimed investigate association between infant GMC temperament. The study...
ABSTRACT A 613-bp fragment of an essential ketosynthase gene from the biosynthetic pathway aromatic polyketide antibiotics was sequenced 99 actinomycetes isolated soil. Phylogenetic analysis showed that isolates clustered into clades correspond to various classes polyketides. Additionally, sequencing a 120-bp γ-variable region 16S ribosomal DNA (rDNA) and subsequent comparative sequence revealed incongruity between rDNA phylogenetic trees, which strongly suggests there has been horizontal...
The aim of this study was to investigate whether overweight/obese women in metabolic disorder group (MDG, n = 27) differ their gut microbiota composition from non-metabolic (NMDG, 47) and normal weight (NWG, 11). Gut profiled fecal samples by 16S rRNA fluorescence situ hybridization flow cytometry 85 premenopausal women. Body measured bioimpedance, dietary intakes were collected via food diaries. Standard procedures used assess plasma glucose, serum insulin, lipids, inflammatory status. We...
To identify serum biomarkers through metabolomics approach that distinguishes physically inactive overweight/obese women with metabolic syndrome from those who are metabolically healthy, independent of body weight and fat mass.We applied nuclear magnetic resonance spectroscopy-based profiling fasting samples to examine the differences between 78 previously inactive, mass matched premenopausal without MetS. MetS was defined as presence at least three following five criteria: waist...
Maternal prenatal stress associates with infant developmental outcomes, but the mechanisms underlying this association are not fully understood. Alterations in composition and function of intestinal microbiota may mediate some observed health effects, a viewpoint that is supported by animal studies along small human study showing exposure to modifies offspring's microbiota. In current study, we aim investigate associations between maternal psychological distress (PPD) hair cortisol...
The aim of this study was to investigate the association between cardiorespiratory fitness and gut microbiota composition in premenopausal women. participants consisted 71 Finnish women (aged 19-49 years). Gut were analyzed using flow cytometry, 16S rRNA gene hybridization DNA-staining. Maximum oxygen uptake (VO2max) assessed by respiratory gas analyzer body Bioimpdance. We found that with low VO2max had lower Bacteroides, but higher Eubacterium rectale-Clostridium coccoides than high group...
Abstract Objective To investigate the long-term effects of duration postpartum lactation on maternal body composition and risk for cardio-metabolic disorders in later life. Design Retrospective study. Body was measured using dual-energy X-ray absorptiometry serum glucose, insulin lipids were analysed enzymatic photometric methods 16–20 years after last pregnancy. Medical history lifestyle factors collected via a self-administered questionnaire. Detailed information regarding weight change...
This study aimed at establishing bacterial flagellin-recognizing toll-like receptor 5 (TLR5) as a novel link between gut microbiota composition, adipose tissue inflammation, and obesity. An microarray database was used to compare women having the highest (n = 4, H-TLR) lowest L-TLR) expression levels of TLR5-signaling pathway genes. Gut composition profiled using flow cytometry FISH. Standard laboratory techniques were determine anthropometric clinical variables. In vivo results verified...
Several studies have reported that the intestinal microbiota composition of celiac disease (CD) patients differs from healthy individuals. The possible role gut in pathogenesis is, however, not known. Here, we aimed to assess differences early fecal between children later developed CD and controls matched for age, sex HLA risk genotype.We used 16S rRNA gene sequencing examine 27 with high genetic developing CD. Nine these by age 4 years. Stool samples were collected at 9 12 months, before...
Although prostate cancer (PCa) is the most common in men Western countries, there significant variability geographical incidence. This might result from genetic factors, discrepancies screening policies, or differences lifestyle. Gut microbiota has recently been associated with progression, but its role PCa unclear. Characterization of gut and functions PCa. In a prospective multicenter clinical trial (NCT02241122), profiles 181 suspicion were assessed utilizing 16S rRNA sequencing....
Objectives To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI). Materials and methods Nonbacterial CPI was induced Wistar strain with subcutaneous testosterone 17β‐oestradiol (E 2 ) hormone pellets for 18 weeks. Rats placebo served as healthy controls. were stratified into two groups, which drank either plain tap water (control group) or supplemented 2% galactoglucomannan‐rich hemicellulose extract ( GGM from...
Understanding the importance of gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised hope for therapeutic microbes. We have shown that high hepatic fat content associated with low abundance Faecalibacterium prausnitzii humans and, further, administration F. prevented NAFLD mice. Here, we aimed at targeting by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD. First, effect XOS on growth was assessed vitro. Then, supplemented or not (HFD, 60% energy from fat) (LFD)...
Human brain and intestinal microbes reportedly maintain a constant bidirectional connection through diverse neural, endocrine, immune, metabolic pathways. Increasing evidence indicates that this communication system, referred to as microbiota-gut-brain axis, enables the gut influence several aspects of function behavior, including hypothalamic-pituitary-adrenal (HPA) axis stress responses, on other hand, can affect microbiota. However, role microbiota in HPA functioning humans remains be...
Abstract A better understanding of how bone growth is regulated during peripuberty important for optimizing the attainment peak mass and prevention osteoporosis in later life. In this report we used hierarchical models to evaluate associations insulin-like factor 1 (IGF-1), estradiol (E2), testosterone (T) with peripubertal a 7-year longitudinal study. Two-hundred fifty-eight healthy girls were assessed at baseline (mean age 11.2 years) 1, 2, 3.5, 7 years. Serum concentrations IGF-1, E2, T...