A5045557464- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Epigenetics and DNA Methylation
- Gastrointestinal motility and disorders
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Barrier Structure and Function Studies
- Vibrio bacteria research studies
- Fecal contamination and water quality
- Helicobacter pylori-related gastroenterology studies
Merck & Co., Inc., Rahway, NJ, USA (United States)
2024
McGill University
2016-2021
Stunting, a severe and multigenerational growth impairment, globally affects 22% of children under the age 5 years. Stunted have altered gut bacterial communities with higher proportions Proteobacteria, phylum several known human pathogens. Despite links between an microbiota stunting, role bacteriophages, highly abundant viruses, is unknown. Here, we describe bacteriophage Bangladeshi stunted younger than 38 months. We show that these harbor distinct bacteriophages relative to their...
ABSTRACT Bacterial communities are highly complex, with interaction networks dictating ecosystem function. interactions constrained by the spatial organization of these microbial communities, yet studying at single-cell level has been technically challenging. Here, we use recently developed high-phylogenetic-resolution microbiota mapping fluorescence in situ hybridization technology to image gut species and level. We simultaneously 63 different bacterial spatially characterize perturbation...
Changes in bacterial diversity the human gut have been associated with many conditions, despite not always reflecting changes activity. Methods linking identity to function are needed for improved understanding of how communities adapt and respond their environment, including gut. Here, we optimized bioorthogonal non-canonical amino acid tagging (BONCAT) microbiota combined it fluorescently activated cell sorting sequencing (FACS-Seq) identify translationally active members community. We...
While the diversity of human gut microbiota is becoming increasingly well characterized, bacterial physiology still a critical missing link in understanding how may be implicated disease. The current best practice for studying involves immediate storage fecal samples an anaerobic chamber. This reliance on access to chambers greatly limits scope sample populations that can studied. Here, we assess effects short-term oxygen exposure and diversity. We use relative nucleic acid content membrane...
Longitudinal studies on the gut microbiome that follow effect of a perturbation are critical in understanding microbiome's response and succession to disease. Here, we use dextran sodium sulfate (DSS) mouse model colitis as tractable study how bacteria change their physiology over course perturbation. Using single-cell methods such flow cytometry, bioorthogonal noncanonical amino acid tagging (BONCAT), population-based cell sorting combined with 16S rRNA sequencing, determine diversity...
Abstract Background Changes in bacterial diversity the human gut microbiome, characterized primarily though DNA sequencing methods, have been associated with many different adverse health conditions. However, these changes do not always reflect activity, and thus how microbiome is implicated disease still often understood. New methods that link together function to identity are needed further explore role of disease. We optimized bioorthogonal non-canonical amino acid tagging (BONCAT) for...