A5053277089- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Extraction and Separation Processes
- Environmental Policies and Emissions
- Nanoplatforms for cancer theranostics
- Enzyme Structure and Function
- Healthcare professionals’ stress and burnout
- Intimate Partner and Family Violence
- 3D Printing in Biomedical Research
- Workplace Violence and Bullying
- Microbial Metabolic Engineering and Bioproduction
- HIV/AIDS drug development and treatment
- Nanoparticle-Based Drug Delivery
- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- Cancer Cells and Metastasis
- Acute Lymphoblastic Leukemia research
- Radiation Therapy and Dosimetry
- Bacteriophages and microbial interactions
- Endoplasmic Reticulum Stress and Disease
SciencePharma (Poland)
2021-2024
Medical University of Warsaw
2023
University of Oslo
2023
Gdańsk Medical University
2023
University of Gdańsk
2023
Gdańsk University of Technology
2012-2023
Ashkelon Academic College
2022
Barzilai Medical Center
2022
Institute of Cytology and Genetics
2016
Siberian Branch of the Russian Academy of Sciences
2016
Poor efficiency of chemotherapeutics in the eradication Cancer Stem Cells (CSCs) has been driving search for more active and specific compounds. In this work, we show how cell density-dependent stage culture profiles can be used drug development workflows to achieve robust activity (IC50 EC50) results. Using flow cytometry light microscopy, characterized cytological HL-60-, A-549-, HEK-293-derived sublines with a focus on their primitive content. We then range cytotoxic substances—C-123,...
Workplace violence (WPV) against healthcare workers, a serious public health problem with profound implications, has worsened during the COVID-19 pandemic. This study examined incidence of different types WPV in hospital Israel pandemic and analyzes factors associated its occurrence. A cross-sectional was performed via an online questionnaire 486 workers at government Israel. Data were collected about sociodemographic occupational characteristics, exposure to forms over preceding six months,...
Seven conjugates composed of well-known fluoroquinolone antibacterial agents, ciprofloxacin (CIP) or levofloxacin (LVX), and a cell-penetrating peptide transportan 10 (TP10-NH2) were synthesised. The drugs covalently bound to the via an amide bond, methylenecarbonyl moiety, disulfide bridge. Conjugation fluoroquinolones TP10-NH2 resulted in congeners demonstrating antifungal vitro activity against human pathogenic yeasts Candida genus (MICs 6.25–100 µM range), whereas components poorly...
Hematopoietic cell transplantation (HCT) is often considered a last resort leukemia treatment, fraught with limited success due to microbial infections, leading cause of mortality in patients. To address this critical issue, we explored novel approach by synthesizing antileukemic agents containing antibacterial substances. This innovative strategy involves conjugating fluoroquinolone antibiotics, such as ciprofloxacin (CIP) or levofloxacin (LVX), the cell-penetrating peptide transportan 10...
The fusion of penetrating peptides (PPs), e.g., cell penetration (CPPs), or antimicrobial (AMPs) together with agents is an expanding research field. Specific AMPs, such as lactoferricin B (LfcinB), have demonstrated strong antibacterial, antifungal, and antiparasitic activity, well valuable anticancer proving beneficial in the development conjugates. resulting conjugates offer potential dual functionality, acting both agent. This especially necessary cancer treatment where microbial...
Glioblastoma is a leader among the most malignant brain tumors with average lifespan of patients around 9-12 months. For prevention and treatment neuropathology, variety therapeutic surgical approaches are being developed improved, including radiation chemical therapy methods. In our work, we investigated cytopathic effect X-ray irradiation application metal oxides nanoparticles such as manganese oxide (MnO) on U87 human glioblastoma cells. We used dose 0.5, 4, 40 100 Gy in combination at...
Consistent with a role in catalyzing rate-limiting step of protein folding, removal genes encoding cytoplasmic folding catalysts belonging to the family peptidyl-prolyl cis/trans isomerases (PPIs) Escherichia coli confers conditional lethality. To address molecular basis essentiality PPIs, multicopy suppressor approach revealed that overexpression chaperones (DnaK/J and GroL/S), transcriptional factors (DksA SrrA), replication proteins Hda/DiaA, asparatokinase MetL, Cmk acid resistance...
Protein folding often requires molecular chaperones and catalysts, such as peptidyl-prolyl cis/trans isomerases (PPIs). The Escherichia coli cytoplasm contains six well-known PPIs, although a requirement of their PPIase activity, the identity substrates relative enzymatic contribution is unknown. Thus, strains lacking all periplasmic one cytoplasmic PPIs were constructed. Measurement activity revealed that PpiB major source in cytoplasm. Furthermore, viable Δ6ppi could be constructed only on...
The fusion of penetrating peptides (PPs), e.g., cell penetration (CPPs) or antimicrobial (AMPs), together with agents is an expanding research field. Specific AMPs, such as lactoferricin B (LfcinB), have demonstrated strong antibacterial, antifungal, and antiparasitic activity, well valuable anticancer proving beneficial in the development conjugates. resulting conjugates offer potential dual functionality, acting both agent. This especially necessary cancer treatment, where microbial...
The fusion of penetrating peptides (PPs) e.g., cell penetration (CPP) or antimicrobial (AMPs) together with aniti-micriobial agents is an expanding research field. Specific AMPs, such as Lactoferricin B (LfcinB), have demonstrated strong antibacterial, anti-fungal and antiparasitic activity, well valuable anticancer proving beneficial in the development conjugates. resulting conjugates offer potential dual functionality, acting both agent. This particularly important treating leukemia...