A5044429664- Enzyme Catalysis and Immobilization
- T-cell and B-cell Immunology
- Microbial Metabolic Engineering and Bioproduction
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Biofuel production and bioconversion
- Enzyme-mediated dye degradation
- Plant biochemistry and biosynthesis
- Chromium effects and bioremediation
- Sugarcane Cultivation and Processing
- Steroid Chemistry and Biochemistry
- Electrochemical sensors and biosensors
- Pharmacogenetics and Drug Metabolism
- Biochemical and biochemical processes
- Lignin and Wood Chemistry
- Enzyme Structure and Function
Canada's Michael Smith Genome Sciences Centre
2023-2024
University of British Columbia
2017-2021
Vancouver Biotech (Canada)
2019
Abstract The recalcitrance of woody biomass, particularly its lignin component, hinders sustainable transformation to fuels and biomaterials. Although the recent discovery several bacterial ligninases promises development novel biocatalysts, these enzymes have largely been characterized using model substrates: direct evidence for their action on biomass is lacking. Herein, we report delignification by a small laccase (sLac) from Amycolatopsis sp . 75iv3. Incubation steam-pretreated poplar...
The development of microbial cell factories requires robust synthetic biology tools to reduce design uncertainty and accelerate the design-build-test-learn process. Herein, we developed a suite integrative genetic facilitate engineering Rhodococcus, genus bacteria with considerable biocatalytic potential. We first created pRIME, modular, copy-controlled integrative-vector, provide platform for strain characterizing parts. This vector was then employed benchmark series strong promoters. found...
Lipid accumulation in<italic>Rhodococcus jostii</italic>RHA1 was re-wired through heterologous pathway engineering to create an industrially-viable biocatalyst for the sustainable production of high-value wax esters.
Many rhodococci are oleaginous and, as such, have considerable potential for the sustainable production of lipid-based commodity chemicals. Herein, we demonstrated that Rhodococcus jostii RHA1, a soil bacterium catabolizes wide range organic compounds, produced wax esters (WEs) up to 0.0002% its cellular dry weight during exponential growth on glucose. These WEs were fully saturated and contained primarily 31 34 carbon atoms. Moreover, they present at higher levels than under...
Steroid-degrading bacteria, including Mycobacterium tuberculosis (Mtb), utilize an architecturally distinct subfamily of acyl coenzyme A dehydrogenases (ACADs) for steroid catabolism. These ACADs are α2β2 heterotetramers that usually encoded by adjacent fadE-like genes. In mycobacteria, ipdE1 and ipdE2 (formerly fadE30 fadE33) occur in divergently transcribed operons associated with the catabolism 3aα-H-4α(3′-propanoate)-7aβ-methylhexahydro-1,5-indanedione (HIP), a metabolite. smegmatis,...
Current tools for functionally profiling T cell receptors with respect to cytotoxic potency and cross-reactivity are hampered by difficulties in establishing model systems test these proteins the contexts of different HLA alleles against broad arrays potential antigens. We have implemented a granzyme-activatable sensor cytotoxicity universal prototyping platform which enables facile recombinant expression any combination TCR-, peptide-, class I MHC-coding sequences direct assessment...
Current tools for functionally profiling T cell receptors with respect to cytotoxic potency and cross-reactivity are hampered by difficulties in establishing model systems test these proteins the contexts of different HLA alleles against broad arrays potential antigens. We have implemented validated a granzyme-activatable sensor cytotoxicity novel universal prototyping platform which enables facile recombinant expression any combination TCR-, peptide-, class I MHC-coding sequences direct...
Abstract Current tools for functionally profiling T cell receptors with respect to cytotoxic potency and cross-reactivity are hampered by difficulties in establishing model systems test these proteins the contexts of different HLA alleles against broad arrays potential antigens. We have implemented validated a granzyme-activatable sensor cytotoxicity novel universal prototyping platform which enables facile recombinant expression any combination TCR-, peptide-, class I MHC-coding sequences...