A5103142716- RNA and protein synthesis mechanisms
- Gut microbiota and health
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Prenatal Screening and Diagnostics
- CRISPR and Genetic Engineering
- Dermatology and Skin Diseases
- Fetal and Pediatric Neurological Disorders
- RNA Research and Splicing
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- Complementary and Alternative Medicine Studies
- Meat and Animal Product Quality
- CAR-T cell therapy research
- Metabolomics and Mass Spectrometry Studies
- Genomic variations and chromosomal abnormalities
- Machine Learning in Bioinformatics
- Psoriasis: Treatment and Pathogenesis
- Antibiotic Resistance in Bacteria
- Probiotics and Fermented Foods
- MicroRNA in disease regulation
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
Eurofins (Germany)
2018-2024
GATC Biotech (Germany)
2012-2019
Technical University of Munich
2006
The main source of error in gene expression is messenger RNA decoding by the ribosome. Translational accuracy has been suggested on a purely correlative basis to positively coincide with maximum possible life span among different rodent species, but causal evidence that translation errors accelerate aging vivo and limit lacking. We have now addressed this question experimentally creating heterozygous knock-in mice express ribosomal ambiguity mutation RPS9 D95N, resulting genome-wide...
Objective The objective of this study is to validate the diagnostic accuracy a non-invasive prenatal test for detecting trisomies 13, 18, and 21 population in Germany Switzerland. Methods Random massively parallel sequencing was applied using Illumina platform HiSeq2000. Fetal aneuploidies were identified median absolute deviation based z-score equation. A bioinformatics algorithm on guanine-cytosine normalization after data unblinded. Results invasive procedures compared. Overall, 40/42...
Klebsiella pneumoniae is responsible for nosocomial infections causing significant morbidity and mortality. Treatment of newly emerging multi-drug resistant strains hampered due to severely limited antibiotic choices. Passive immunization targeting LPS O-antigens has been proposed as an alternative therapeutic option, given the variability O-antigens. Here we report that O3 serogroup, previously considered have uniform O-antigen built mannan, represents three different subtypes differing in...
Abstract Background Availability of high-resolution RNA crystal structures for the 30S and 50S ribosomal subunits subsequent validation comparative secondary structure models have prompted biologists to use three-dimensional (rRNA) evaluating sequence alignments rRNA genes. Furthermore, tertiary structural features are highly useful successfully employed in designing targeted oligonucleotide probes intended situ hybridization experiments. RNA3D, a program combine alignment information with...
The complex smear microbiota colonizing the surface of red-smear cheese fundamentally impacts ripening process, appearance and shelf life cheese. To decipher prokaryotic composition microbiome, a semi-hard ripened was studied post-ripening by culture-based culture-independent molecular approaches. aim to detect potential bacterial alterations in resulting from storage vacuum film-prepackaging, which is often accompanied development defect. Next-generation sequencing amplified 16S rRNA gene...
Biological agents used to treat severe psoriasis may alter the gut microbiota, though current knowledge is limited. This study examines whether switching from TNFα inhibitors, which patients had reduced or lost effect, brodalumab, an IL-17 inhibitor, affects microbiota in with and how these changes correlate clinical variables of severity depressive symptoms. Fecal samples were collected before treatment switch 12 weeks after analyzed for composition using next-generation sequencing...
Polynucleobacter asymbioticus strain QLW-P1DMWA-1T represents a group of highly successful heterotrophic ultramicrobacteria that is frequently very abundant (up to 70% total bacterioplankton) in freshwater habitats across all seven continents. This was originally isolated from shallow Alpine pond characterized by rapid changes water temperature and elevated UV radiation due its location at an altitude 1300 m. To elucidate the strain's adjustment fluctuating environmental conditions, we...
Process intensification is increasingly used in the mammalian biomanufacturing industry. The key driver of this trend need for more efficient and flexible production strategies to cope with increased demand biotherapeutics predicted next years. Therefore, such intensified should be designed, established, characterized. We established a CHO cell process consisting an fed-batch (iFB), which inoculated by N-1 perfusion that reaches high concentrations (100 × 106 c ml-1 ). investigated impact...
The gut microbiota plays a critical role in immune system function, with dysbiosis linked to systemic inflammation, contributing conditions like psoriasis and depression. Although biological treatments for severe are known impact bacteria, less is understood about their effects on fungi. This study aims investigate fungal changes patients transitioning from TNF-α inhibitors brodalumab. Fecal samples 20 were analyzed using Illumina MiSeq sequencing of the ITS2 region 18S rRNA. Microbial...
Noninvasive prenatal detection of fetal aneuploidies using cell free DNA from maternal plasma has recently been shown to be feasible by massively parallel sequencing (MPS). Here, we describe a large-scale clinical study, which will finished in spring 2012. The aim the study is validate diagnostic accuracy our noninvasive test based on MPS for detecting aneuploidies. Maternal blood samples were collected more than 500 pregnant women prior invasive procedures at different clinics located...