A5033968393- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Cell Image Analysis Techniques
- Functional Brain Connectivity Studies
- Veterinary Pharmacology and Anesthesia
- Neurological disorders and treatments
- Advanced Vision and Imaging
- Traumatic Brain Injury and Neurovascular Disturbances
- Axon Guidance and Neuronal Signaling
- Neurotransmitter Receptor Influence on Behavior
- Ultrasound Imaging and Elastography
- Neuroendocrine regulation and behavior
- Barrier Structure and Function Studies
- Silk-based biomaterials and applications
- Animal testing and alternatives
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neural Engineering
- Memory and Neural Mechanisms
- Pain Mechanisms and Treatments
- Medical Imaging Techniques and Applications
- Scientific Computing and Data Management
- Neuroscience and Neuropharmacology Research
Harvard University
2015-2023
Howard Hughes Medical Institute
2015-2021
Inscopix (United States)
2021
McGill University Health Centre
2011
In vivo calcium imaging through microendoscopic lenses enables of previously inaccessible neuronal populations deep within the brains freely moving animals. However, it is computationally challenging to extract single-neuronal activity from data, because very large background fluctuations and high spatial overlaps intrinsic this recording modality. Here, we describe a new constrained matrix factorization approach accurately separate then demix denoise signals interest. We compared proposed...
In probabilistic and nonstationary environments, individuals must use internal external cues to flexibly make decisions that lead desirable outcomes. To gain insight into the process by which animals choose between actions, we trained mice in a task with time-varying reward probabilities. our implementation of such two-armed bandit task, thirsty information about recent action action–outcome histories two ports deliver water probabilistically. Here comprehensively modeled choice behavior...
Highlights•Silk/AAV films deposited on implants lead to localized, aligned expression•Silk/AAV facilitates targeting small nuclei for optogenetic experiments•Silk/AAV coatings allow continuous expression along tapered optical fibers•Widespread can be achieved by coating cranial windows with silk/AAVSummaryOptical methods of interrogating neural circuits have emerged as powerful tools understanding how the brain drives behaviors. Optogenetic proteins are widely used control neuronal activity,...
Abstract The lack of sensitive and robust behavioral assessments pain in preclinical models has been a major limitation for both research the development novel analgesics. Here, we demonstrate data acquisition analysis platform that provides automated, quantitative, objective measures naturalistic rodent behavior an observer-independent unbiased fashion. technology records freely behaving mice, dark, over extended periods continuous 2 parallel video streams: (1) near-infrared frustrated...
Here we describe the development and application of miniature integrated microscopes (miniscopes) paired with microendoscopes that allow for visualization manipulation neural circuits in superficial subcortical brain regions freely behaving animals. Over past decade miniscope platform has expanded to include simultaneous optogenetic capabilities, electrically-tunable lenses enable multi-plane imaging, color-corrected optics, an data acquisition streamlines multimodal experiments. Miniscopes...
Scientists are adopting new approaches to scale up their activities and goals. Progress in neurotechnologies, artificial intelligence, automation, tools for collaboration promises bursts of discoveries. However, compared other disciplines the industry, neuroscience laboratories have been slow adopt key technologies support collaboration, reproducibility, automation. Drawing on progress fields, we define a roadmap implementing automated research workflows diverse teams. We propose...
In vivo calcium imaging through microscopes has enabled deep brain of previously inaccessible neuronal populations within the brains freely moving subjects. However, microendoscopic data suffer from high levels background fluorescence as well an increased potential for overlapping signals. Previous methods fail in identifying neurons and demixing their temporal activity because cellular signals are often submerged large fluctuating background. Here we develop efficient method to extract with...
Establishing precise synaptic connectivity during development is crucial for neural circuit function. However, very few molecules have been identified that are involved in determining where and how many synapses form. The Plexin cell-surface a conserved family of axon guidance receptors mediate fasciculation repulsion development, later PlexinA eliminating axonal branches synapse numbers. Here we investigate the roles PlexinB branch varicosity formation Drosophila. We knocked down or...
Abstract In probabilistic and nonstationary environments, individuals must use internal external cues to flexibly make decisions that lead desirable outcomes. To gain insight into the process by which animals choose between actions, we trained mice in a task with time-varying reward probabilities. our implementation of such “two-armed bandit” task, thirsty information about recent action action-outcome histories two ports deliver water probabilistically. Here, comprehensively modeled choice...
SUMMARYThe lack of robust quantifiable behavioral assessments pain in pre-clinical models, especially ongoing pain, has been a major limitation for both neurobiology research and development novel analgesics. Current methods typically require extensive animal-human observer interaction are often followed by manual, thus subjective, scorings, which susceptible to bias variability, labor-intensive time-consuming. Here we demonstrate that, leveraging modern machine vision learning tools,...
Healthy brain function requires a delicate balance between neural activity and local vascular dynamics. Perturbations to this balance, such as vessel diameter changes or fluctuations in red blood cell velocity, are thought underlie number of neurodegenerative neurovascular diseases, including migraine, stroke, epilepsy. Here, for the first time, we demonstrate high fidelity simultaneous acquisition circuit dynamics freely-moving animals using our newly developed nVue dual color miniscope....