A5013389044- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Complement system in diseases
The University of Texas at Austin
2017-2020
Accurately measuring antibody repertoire sequence composition in a small amount of blood is challenging yet important for understanding responses to infection and vaccination. We develop molecular identifier clustering-based immune sequencing (MIDCIRS) use it study age-related development diversification before during acute malaria infants (< 12 months old) toddlers (12-47 with 4-8 ml blood. Here, we show this accurate high-coverage repertoire-sequencing method can as few 1000 naive B cells....
Follicular helper CD4+ T cells (TFH) play an integral role in promoting B cell differentiation and affinity maturation. Whereas TFH frequencies are increased lymph nodes (LNs) from individuals infected with HIV, humoral immunity remains impaired during chronic HIV infection. Whether inhibits responses LNs unclear. Advances this area have been limited by the difficulty of accessing human lymphoid tissues. Here, we combined high-dimensional mass cytometry receptor repertoire sequencing to...
CXCR5 is a key marker of follicular helper T (TFH) cells. Using primary lymph nodes (LNs) from HIV-infected patients, we identified population CXCR5− CD4+ cells with TFH-cell-like features. This subset becomes expanded in severe HIV infection and characterized by the upregulation activation markers high PD-1 ICOS surface expression. Integrated analyses on phenotypic heterogeneity, functional capacity, cell receptor (TCR) repertoire, transcriptional profile, epigenetic state CXCR5−PD-1+ICOS+...
Abstract The Ab response to HIV is of great interest, particularly in the context a protective vaccine and broadly neutralizing Abs, but research typically geared toward elite controllers because their ability successfully control virus. In this study, we studied evolution repertoire over first year infection people classified as rapid progressors (RP) compared with typical progressors. RPs are an important yet understudied group patients by decline CD4 counts accelerated development AIDS....
ABSTRACT Accurately measuring antibody repertoire sequence composition in a small amount of blood is challenging yet important to the understanding response infections and vaccinations. Here, we describe an accurate high-coverage sequencing method, MIDCIRS, which uses as few 1,000 naïve B cells. Using it, studied age-related development diversification before during acute malaria infants (< 12 months old) toddlers (12 – 47 with 4-8 ml draws. Unexpectedly, discovered high levels somatic...
Summary CXCR5 is a key surface marker expressed on follicular helper T (T FH ) cells. We report here B cell help functionality in population of CD4 + cells isolated from primary human lymph nodes (LN) that lacked expression. This - subset distinguished other by high PD-1 Accumulation correlated with peripheral depletion and an increase T-bet the LN, highlighting these atypical as component lymphoid dysregulation during chronic HIV infection. By interrogating phenotypic heterogeneity,...