Aphelenchoides fragariae, commonly known as strawberry crimp nematodes, primarily target the aerial parts of plants, affecting both internal and external structures. In Indonesia, where strawberries are predominantly cultivated in highland regions, presence disease has been confirmed. Infected plants exhibit symptoms such stunted growth, reddened foliage, crimped or curled leaves, malformed buds blooms. Aboveground damage caused by nematodes includes contorted shoots, undersized reddish...

Normal 0 false IN X-NONE Begomovirus infection has a significant impact of lowering chilli yield in Indonesia. A constraint narrow genetic variability Indonesia made the mutation breeding program as solution worth-pursuing increasing variability. The objective this study was to determine LD 50 point for each five irradiated genotypes and optimum dose gamma irradiation inducing resistance other improved agronomical traits. conducted Indonesian Vegetable Research Institute (IVEGRI) at...

ABSTRACT Two begomoviruses (Java virus-1 and Java virus-2), two satellite DNAs (DNAbeta01 DNAbeta02), a recombinant DNA (recDNA) were cloned from single tomato plant Indonesia with leaf curl symptoms, the role of these in etiology begomovirus disease was investigated. The genome organizations viruses similar to those other Old World monopartite begomoviruses. Comparison sequences revealed that newly described virus for which name Tomato (ToLCJAV) is proposed. virus-2 strain Ageratum yellow...

High incidence of Pepper yellow leaf curl virus (PepYLCV) was observed in Indonesia since early 2000. Disease Yogyakarta, Central and West Java reached 100% on Capsicum frutescens, but only 10-35% C. annuum. As an exception, the disease annuum cv. TM 999 range 70-100%. The causal agent disease, PepYLCV, detected by polymerase chain reaction. Viral specific DNA fragment size ∼1600 bp ∼550 amplified from infected plants using two pairs geminivirus universal primers pAL1v1978/pAL1c715,...

Twenty‐four isolates of Chilli veinal mottle virus (ChiVMV) from China, India, Indonesia, Taiwan and Thailand were analysed to determine their genetic relatedness. Pathogenicity was confirmed by induction systemic mosaic and/or necrotic ringspot symptoms on Capsicum annuum after mechanical inoculation. The 3′ terminal sequences the viral genomic RNA determined. coat protein (CP) coding regions ranged 858 864 nucleotides untranslated (3′UTR) 275 289 in length. All had inverted repeat sequence...

Background and Objective: Squash mosaic virus (SqMV) is a seed-borne infecting Cucurbitaceae, including cucumber plants.The SqMV cause systemic infections in plants it very difficult to find the effective control strategies reduce its spread.Chitosan Plant Growth Promoting Rhizobacteria (PGPR) has been reported suppress plant diseases.The objective of this study was determine potential chitosan PGPR disease intensity caused by enhance growth on cucumbers.Methodology: Cucumber cultivars first...

Yellowing and leaf curl symptoms were observed in tomato pepper fields near Bogor, Java, Indonesia 2000. Samples collected from one diseased (Lycopersicum esculentum) three chili (Capsicum annuum) plants. Viral DNA was extracted (2) tested for the presence of geminiviral DNA-A, DNA-B, associated satellite using polymerase chain reaction (PCR) with previously described primers (1,3,4). The begomovirus DNA-A general primer pair PAL1v1978/PAR1c715 amplified predicted 1.4-kb fragment two...

Downy mildew is very detrimental disease of maize production in Indonesia. Adequate information regarding the identification key based on morphological and morphometric characteristic causal fungi downy Indonesia limited. Study for detection morphological, morphometric, moleculer base urgently required. Artificial sporulation induction method performed to obtain morphology fungus as a whole.The were morphologically identified symptomatic collected from 13 provinces Indonesia.Three species,...

Bean dwarf mosaic geminivirus (BDMV) was shown to have a bipartite genome (DNA-A and DNA-B) from restriction map sequence data comparisons of cloned DNAs. The nucleotide sequences the infectious clones DNA-A DNA-B were determined. Computer analyses showed that BDMV resembles other whitefly-transmitted geminiviruses. (2,615 nt) (2,575 little homology than within common region []

Perkembangan infeksi <em>Begomovirus </em>pada cabai di Indonesia tidak menutup kemungkinan adanya isolat baru yang berbeda gejala dan kisaran inangnya. Tujuan penelitian adalah untuk melakukan identifikasi </em>cabai dari beberapa sentra produksi berdasarkan Penelitian dilakukan laboratorium rumah kasa virologi Balai Tanaman Sayuran (BALITSA) Lembang, Bulan Mei 2013 sampai dengan Agustus 2013. Isolat diperoleh enam daerah, yaitu: Leuwikopo-Bogor, Brebes, Magelang, Kediri,...

Yellow leaf curl disease in chili pepper has been reported Bali Island since the early 2012. Research was conducted to identify virus causing this and distribution Bali. Field survey carried out observe intensity collect field samples from several growing areas (Karangasem, Bangli, Tabanan, Gianyar). Begomovirus identification then by polymerase chain reaction method using universal primers SPG1/SPG2, followed an analysis of amplified target DNA sequences. The incidence yellow reached 100%...

Selection criteria for developing Phytophthora blight-resistant varieties of pepper had been done in both laboratory and fi  eld conditions. Resistance screening were conducted on 28-days-old plants grown 72-cell fl  ats by inoculating 5 mL inoculum (contain 105 zoospore mL-1) to the base each plant. capsici isolate used this experiment was TG01, which then identifi  ed as race-3. The evaluation genotypes characteristics eld.  Eight characters demonstrated a wide genetic variability; those...

Begomovirus was identified as one of the causal agents causing yellow mosaic disease on yard long bean (Vigna unguiculata subsp. sesquipedalis L.) in Java. Research conducted to characterize virus based its nucleotide sequences. started by field survey collect leaf samples, followed detection using polymerase chain reaction and analysis Samples from Tegal, Klaten, Magelang, Cirebon, Subang, Bogor were positively infected specific viral DNA amplification. Sequence indicated that infecting...

One of factors that affect the severity plant diseases caused by virus is time Begomovirus infection. There a tendency younger plants are infected, greater damage infection will be. This study aimed to reveal exact for purpose resistance selection and determine onset which resulted in most devastating plant. The was conducted factorial randomized completely block design with two factors. first factor 5 chili pepper genotypes second at three phases no as control treatment. results showed...

Background and Objective: Anthracnose disease on chili pepper caused by Colletotrichum spp. is known as one of the devastated world-wide including Indonesia.This study was carried out to identify species recovered from fruits with anthracnose symptoms collected some areas in Indonesia through morphological molecular approaches.Materials Methods: Infected fruit samples showed symptom several were isolated purified using single spore methods.All isolates then characterized based conidial shape...

Yellow leaf curl disease of chili pepper caused by Pepper yellow virus (PYLCV) has been reported as an important in Java and Bali. Disease severity reached 80−100% it may cause significant yield losses. In order to reduce the negative impact synthetic insecticides, silica application was evaluated for its potency suppress disease. A greenhouse experiment conducted using randomly block design with 2 factors: PYLCV isolate (Java Bali) (SiO2) treatment (with without). Parameters observed were...

ABSTRACTIdentification of the causal agent yellow leaf curl disease on cucumbers. Yellow has been reported to cause serious diseases and yield losses tobacco, chilli pepper, tomato plants in Java. Similar symptoms were observed recently cucumber from several growing areas West Java (Bogor), Central (Tegal Sukoharjo), Yogyakarta (Sleman). Symptom variations including mosaic, chlorotic spotting, curling, blistering, vein banding, reduction distortion fruit observed. Serological detection using...

Abstract We report that several begomoviruses are associated with tomato leaf curl disease in Java, Indonesia. Tomato plants symptoms were collected from Bandung (west Java), Purwokerto (central Magelang Java) and Malang (east of Indonesia, the major tomato‐growing areas country. Viruses detected using polymerase chain reaction (PCR), universal primers for genus Begomovirus . PCR‐amplified fragments cloned sequenced. Based on sequence comparisons phylogenetic analyses, viruses divided into...

Yellow leaf curl disease of pepper has become an emerging important in West Sumatra since early 2000. Several attempts have been made, including survey and detection, order to identify the causal agent disease. Pepper (Capsicum annuum) plants showing yellow from were analyzed for presence Begomovirus employing Polymerase Chain Reaction (PCR) with degenerate primers pAL1v 1978 pARc 715. A DNA fragment 1.6 kb was successfully amplified subjected direct sequencing. stem loop region found...

<p>Bawang merah umumnya diperbanyak secara vegetatif menggunakan umbi. Bibit unggul bawang ditentukan<br />antara lain oleh status kesehatan benihnya termasuk bebas dari infeksi virus. Penyakit yang disebabkan virus bersifat<br />tular umbi merupakan salah satu kendala dalam meningkatkan produksi merah. Penelitian bertujuan untuk mengetahui<br />jenis-jenis terbawa pada beberapa varietas berasal Jawa Tengah (Brebes) dan Barat<br />(Cirebon, Kuningan, Majalengka,...