A5001657741- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Diabetes and associated disorders
- Immune Response and Inflammation
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- Sexual Differentiation and Disorders
- Neonatal Respiratory Health Research
- Transgenic Plants and Applications
- Veterinary medicine and infectious diseases
- Hepatocellular Carcinoma Treatment and Prognosis
- Glycosylation and Glycoproteins Research
- Helicobacter pylori-related gastroenterology studies
- RNA and protein synthesis mechanisms
- Evolution and Genetic Dynamics
- Toxin Mechanisms and Immunotoxins
- Xenotransplantation and immune response
- Cancer Cells and Metastasis
- Genetic diversity and population structure
Kyoto University
1995-2024
The Graduate University for Advanced Studies, SOKENDAI
2011-2021
Hiroshima University
2021
Pennsylvania State University
2016-2020
Temple University
2016-2020
Evolutionary Genomics (United States)
2020
Nihon University
2010-2019
Kansai Rosai Hospital
2016
University of California, Berkeley
2015
Osaka University
2015
We found that c-kit-positive, lineage marker-negative, Thy-1lo cells are present in both bone marrow and thymus ("BM c-kit" "thymus cells). Although the two cell types phenotypically similar, only BM c-kit showed potential to form colonies vitro as well vivo. However, of them revealed extensive growth differentiation T after direct transfer into an irradiated adult thymus, or a deoxyguanosine-treated fetal thymus. Time course analysis differentiated CD4CD8 double-positive approximately 4 d...
Flow cytometric and immunocytochemical analyses of murine fetal thymus (FT) cells with antibodies to various surface markers transcription factors reveal that the synthesis TCF-1 GATA-3 protein begins simultaneously in a fraction most immature population FT cells, which have phenotype CD4-CD8-CD44+CD25-. No TCF-1-producing is found liver (FL). In CD44+CD25- production immediately followed by intracellular expression CD3 epsilon. It also T cell development from FL, but not FT, progenitors...
We have recently revealed that the thymus is organ showing highest expression of thromboxane (TX) A2 receptor in mice. In this study, thymic cell populations expressing were identified, and effects a TXA2 agonist on these cells examined. Radioligand binding using receptor-specific radioligand single class sites thymocytes with an affinity specificity identical to those reported for receptor. The density was comparable seen blood platelets. This most highly expressed CD4-8- CD4+8+ immature...
Thymic epithelial cells, which create a three-dimensionally organized meshwork structure peculiar to the thymus, develop from simple epithelia of third pharyngeal pouch and cleft during organogenesis. We comparatively investigated thymus anlages normal nude mice by immunohistochemical analysis with regard organization distribution hematopoietic progenitor cells at early stages Our results show that development mouse anlage can be subdivided into least two differences in organization, i.e....
Abstract IL-7 induced the proliferation of normal thymocytes and effect was synergistically potentiated by a small dose IL-2, which itself hardly affected thymocyte proliferation. No synergism observed between any one other lymphokines including IL-1, IL-3, IL-4. The culture stimulated with IL-2 consisted single positive (CD4+CD8- CD4-CD8+) double negative (CD4-CD8-) populations, (CD4+CD8+) cells were completely deleted. Both expressed CD3, but only former exhibited V beta 8 6 in an expected...
The present study was performed to identify cells responsible for the elimination of T reactive with minor lymphocyte-stimulating (Mls) antigens during cell development. Experiments were carried out in a fetal thymus organ culture (FTOC) system. To examine tolerance-inducing activity, various populations from adult CBA/J (Mls-1a) mice injected into deoxyguanosine (dGuo)-treated FTOC C3H/He (Mls-1b) microinjector, and 2 d later, lobes as precursors. After 14 cultivation, harvested assayed...
Patients with 46,XY gonadal dysgenesis (GD) exhibit genital anomalies, which range from hypospadias to complete male-to-female sex reversal. However, a molecular diagnosis is made in only 30% of cases. Heterozygous mutations the human FGFR2 gene cause various craniosynostosis syndromes including Crouzon and Pfeiffer, but testicular defects were not reported. Here, we describe patient whose features would suggest represent new FGFR2-related syndrome, XY reversal or CSR. The was chromosomally...
Embryogenesis in placental mammals is sustained by exquisite interplay between the embryo proper and placenta. UTF1 a developmentally regulated gene expressed both cell lineages. Here, we analyzed consequence of loss during mouse development. We found that homozygous mutant newborn mice were significantly smaller than wild-type or heterozygous mice, suggesting insufficiency caused expression extra-embryonic ectodermal cells at least part contributed to this phenotype. also effects embryonic...
The evolutionary mode of a multi-gene family can change over time, depending on the functional differentiation and local genomic environment members. In this study, we demonstrate such in melanoma antigen (MAGE) gene mammalian X chromosome. MAGE is composed ten subfamilies that be categorized into two types. Type I genes are relatively recent origin, they encode epitopes for human leukocyte (HLA) cancer cells. II ancient some their products known to involved apoptosis or cell proliferation....
Thymus cells of murine fetuses at day 12 gestation are exclusively the CD3-CD4-CD8-CD44+CD25- phenotype, which is known as a hallmark most immature subset thymus cells. In present study, we show that fetal (FT) express Fc gamma RII/ III (FcR) broad range levels on their surface. The FcR+ FT seem to represent T lineage cells, because large majority them specific transcription factors TCF-1 and GATA-3 well CD3 epsilon in cytoplasm. Also shown FcR- population contains progenitors capable...
Abstract Sex chromosomes constantly exist in a dynamic state of evolution: rapid turnover and change heterogametic sex during homomorphic state, often stepping out to heteromorphic followed by chromosomal decaying. However, the forces driving these different trajectories chromosome evolution are still unclear. The Japanese frog Glandirana rugosa is one taxon well suited study on forces. species has two systems, XX‐XY ZZ‐ZW, which separated geographic populations. Both ZZ‐ZW represented 7 (2n...
The East Asian region, including China, Japan and Korea, accounts for half of gastric cancer deaths. However, different areas have contrasting incidences the population structure Helicobacter pylori in this ethnically diverse region is yet unknown. We aimed to investigate genomic differences H. between these identify sequence polymorphisms associated with increased risk. analysed 381 H . genomes collected from three countries using phylogenetic genetic tools characterize differentiation....
We investigated the hemopoietic progenitors in fetal liver (FL) compared with those adult bone marrow (BM) for their ability to differentiate into T, B, and myeloid cells. Lineage marker-negative (Lin-) c-kit+ cells isolated from FL BM were used as source of progenitors. No apparent difference was observed between generate B when these cultured on a monolayer BM-derived stromal cell line. In marked contrast, able T much more rapidly than both vivo intrathymic transfer thymus organ culture...
The mechanism of cell complex formation between lymphocytes and stromal cells was investigated. We found that lymphoid lines both T B lineages could form complexes with from the thymus as well bone marrow but not macrophages or typical fibroblast lines. Formation these is temperature dependent requires presence Mg2+, active cellular metabolism, microfilament assembly cytoskeleton. raised an antiserum against a thymic clone (BATE-2) in rats that, after absorption, this serum effectively block...
Mammalian sex chromosomes originated from a pair of autosomes, and homologous genes on the (gametologs) differentiated through recombination arrest between chromosomes. It was hypothesized that this differentiation in eutherians took place stepwise fashion left footprint X chromosome termed “evolutionary strata.” The evolutionary stratum hypothesis claims strata 1 2 (which correspond to first two steps chromosomal differentiation) were generated stem lineage Theria or before divergence...
Throughout mammalian evolution, recombination between the two sex chromosomes was suppressed in a stepwise manner. It is thought that suppression of led to an accumulation deleterious mutations and frequent genomic rearrangements on Y chromosome. In this article, we review three evolutionary aspects related structures, such as inverted repeats (IRs) palindromes (PDs), chromosomes. First, describe manner which X placental mammals discuss rearrangement might have formation present...
Conditions for the induction of delayed-type hypersensitivity (DTH) in mice to serum proteins ware studied. DTH bovine albumin (BSA) was induced by a subcutaneous immunization with antigen emulsified Freund’s complete adjuvant. The level found be dependent not only on dose but also amount mycobacteria included other under same sensitizing conditions as BSA. Success adoptive transfer lymphoid cells and suppressive effect anti-thymocyte indicated that reaction studied here manifestation...