A5030950947- Advanced Fluorescence Microscopy Techniques
- RNA and protein synthesis mechanisms
- Gene Regulatory Network Analysis
- Photoacoustic and Ultrasonic Imaging
- Advanced biosensing and bioanalysis techniques
- Photochromic and Fluorescence Chemistry
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photoreceptor and optogenetics research
- Advanced Electron Microscopy Techniques and Applications
- Light effects on plants
- Force Microscopy Techniques and Applications
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Electrohydrodynamics and Fluid Dynamics
- Microbial metabolism and enzyme function
- Photonic Crystals and Applications
- Nanopore and Nanochannel Transport Studies
- Lipid Membrane Structure and Behavior
- Luminescence and Fluorescent Materials
- Cell Image Analysis Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Dengue and Mosquito Control Research
- Photodynamic Therapy Research Studies
- Polyamine Metabolism and Applications
- Cellular Automata and Applications
Johns Hopkins University
2022-2023
Johns Hopkins Medicine
2022-2023
Institute for Basic Science
2017-2021
Seoul National University
2012-2020
Seoul Institute
2017-2020
Government of the Republic of Korea
2017
A three-beam femtosecond stimulated Raman scattering (SRS) scheme is formulated and demonstrated to simultaneously induce two different SRS processes associated with Raman-active modes in the same molecule. Two SR gains involving a common pump pulse are coupled compete: As one of Stokes beam intensities increases, other selectively suppressed. We provide theoretical description experimental evidence that selective suppression behavior due limited number photons used for both when an intense...
Abstract We introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-resolution imaging with photon numbers equivalent to the brightest photoswitchable red protein. only fluoresces upon binding fluorogenic metabolite, bilirubin, enabling UV-free reversible easily controllable kinetics and low background under Epi illumination. The on- off-switching rates are controlled by concentration ligand excitation light intensity, respectively, where dissolved...
Abstract Far-field optical nanoscopy has been widely used to image small objects with sub-diffraction-limit spatial resolution. Particularly, reversible saturable fluorescence transition (RESOLFT) photoswitchable fluorescent proteins is a powerful method for super-resolution imaging of living cells low light intensity. Here we demonstrate the first time implementation RESOLFT biological system using organic fluorophores, which are smaller in size and easier be chemically modified. With...
We developed a new pH-sensitive drug delivery carrier based on β-cyclodextrin (β-CD) and 1-methyl-2-(2′-carboxyethyl) maleic anhydrides (MCM). The primary hydroxyl groups of β-CD were successfully attached to MCM residues produce medusa-like β-CD–MCM. residue was conjugated with cephradine (CP) high efficiency ( > 90%). More importantly, β-CD–MCM–CP responded the small pH drop from 7.4 5.5 released greater than 80% drugs within 0.5 h at 5.5. In addition, inclusion complex between adamantane...
We introduce a new, easily implementable sub-diffraction-limit microscopy technique utilizing the optical AND-gate property of fluorescent nanodiamond (FND). demonstrate that when FND is illuminated by two spatially-offset lights different wavelengths, emission comes only from region their overlap, which used to reduce effective point spread function ~300 nm ~130 in lateral plane, well below diffraction limit.
We introduced enhanced UnaG (eUnaG), a ligand-activatable fluorescent protein, for conventional and super-resolution imaging of subcellular structures in the mammalian cells. eUnaG is V2L mutant with twice brighter bulk fluorescence. previously discovered reversible fluorescence switching behavior demonstrated high photon outputs localization numbers single-molecule microscopy (SMLM). In this study, we showed that can be switched off under blue-light illumination, while concentration...
Incorporation of STED into ALEX-FRET increases the concentration limit single-molecule detection by 100-fold to 5 nM.
Summary Stochasticity has emerged as a mechanism to control gene expression. Much of this so-called “noise” been attributed bursting transcription. However, the stochasticity translation not similarly investigated due lack enabling imaging technologies. We developed techniques track single mRNAs and their in live cells for hours, allowing measurement previously uncharacterized dynamics. applied genetic pharmacological perturbations kinetics. Like transcription, is constitutive process but...
Cy5 is one of the most widely used organic dyes with a photoswitching property. It can be reversibly photoconverted to dark state through thiolation primary thiols. Although has been in super-resolution nanoscopy, its mechanism remains unclear. We carried out time-dependent density functional theory calculations investigate excited dynamics and observed site-selective on both ground states. Scanning potential energy surfaces by rotating individual C-C bonds revealed structural similarity...
We report a serendipitous discovery of light-induced generation circular microstructure on glass surface. The has ring shape with notable photophysical properties such as highly bright luminescence and strong resistance to photobleaching. investigated the formation process well micro understand origin this peculiar phenomenon.
In this study, from the point of view ecological systems model, meta path analysis was performed on correlations among peer relationships, anti-bullying, school culture, and social-emotional competence in micro-system. Through this, we suspected significance direct indirect effects between factors towards defending behaviors, by anti-bullying culture environmental variables, mediating social emotional competence, personal variables. To do meta-path conducted domestic academic journals (33...