- Advanced Fluorescence Microscopy Techniques
- Advanced machining processes and optimization
- Metal Alloys Wear and Properties
- Advanced Electron Microscopy Techniques and Applications
- Metallurgy and Material Forming
- Structural Behavior of Reinforced Concrete
- Concrete and Cement Materials Research
- Innovative concrete reinforcement materials
- Photoacoustic and Ultrasonic Imaging
- Nanocluster Synthesis and Applications
- Quantum Dots Synthesis And Properties
- Force Microscopy Techniques and Applications
- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
- Carbon and Quantum Dots Applications
Chung-Ang University
2023
Ulsan National Institute of Science and Technology
2015-2021
Korea Brain Research Institute
2021
The pursuit of exceptionally high photoluminescence (PL) and stability is critical in the development novel fluorophores for use challenging bioimaging optoelectronic devices. Carbon nanodots (CDs) doped with heteroatoms provide a particularly attractive means effectively tailoring their intrinsic properties exploiting new phenomena. Here, we report one-step, scalable synthesis boron-and-nitrogen co-doped CD (BN-CD) outstanding optical unlike those nitrogen-doped (N-CD) solid state as well...
Abstract We introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-resolution imaging with photon numbers equivalent to the brightest photoswitchable red protein. only fluoresces upon binding fluorogenic metabolite, bilirubin, enabling UV-free reversible easily controllable kinetics and low background under Epi illumination. The on- off-switching rates are controlled by concentration ligand excitation light intensity, respectively, where dissolved...
Critical determinants of synaptic functions include subcellular locations, input sources, and specific molecular characteristics. However, there is not yet a reliable efficient method that can detect synapses. Electron microscopy gold-standard to synapses due its exceedingly high spatial resolution. it requires laborious time-consuming sample preparation lengthy imaging time with limited labeling methods. Recent advances in various fluorescence methods have highlighted as substitute for...
Recent development of super-resolution fluorescence imaging technique such as stochastic optical reconstruction microscopy (STORM) and photoactived localization microscope (PALM) has brought us beyond the diffraction limits. It allows numerous opportunities in biology because vast amount formerly obscured molecular structures, due to lack spatial resolution, now can be directly observed. A drawback imaging, however, is that it lacks complete structural information. For this reason, we have...
The contour lathe turning test was performed to evaluate the machinability of PosGRAM70 (eco-friendly, free-cutting steel). A high-speed camera used capture shapes generated chips. with a diameter 30 mm machined into jar-shaped specimens concave and convex contours for different cutting paths, feed rate, depth cut. For comparison, SUM24L (most widely used, but harmful humans by emitting toxic gas during cutting) employed in test. Finely dispersed graphite particles low strength served as...