A5100727690- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Cardiac Ischemia and Reperfusion
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Neuroscience and Neuropharmacology Research
- Genetics, Aging, and Longevity in Model Organisms
- Hepatitis C virus research
- NF-κB Signaling Pathways
- Metabolism and Genetic Disorders
- Influenza Virus Research Studies
- Cytokine Signaling Pathways and Interactions
- Medical Research and Treatments
- Neuroscience and Neural Engineering
- Microbial Metabolites in Food Biotechnology
- Viral Infections and Immunology Research
- ATP Synthase and ATPases Research
- Signaling Pathways in Disease
- Immune Cell Function and Interaction
- Advanced Fluorescence Microscopy Techniques
- Circadian rhythm and melatonin
- Biofuel production and bioconversion
- Neural dynamics and brain function
Pohang University of Science and Technology
2013-2024
Seoul National University
2017-2024
Korea Brain Research Institute
2019-2021
Inje University
2019
Hallym University
2018
University of Toronto
2017
Korea Post
2015
University of Ulsan
2014
Ulsan College
2014
Gyeongsang National University
2013
Abstract Hepatitis C virus (HCV) utilizes autophagy to promote its propagation. Here we show the autophagy-mediated suppression of HCV replication via endoplasmic reticulum (ER) protein SCOTIN. SCOTIN overexpression inhibits and infectious virion production in cells infected with cell culture-derived HCV. nonstructural 5A (NS5A) protein, which is a critical factor for RNA replication, interacts IFN-β-inducible SCOTIN, transports NS5A autophagosomes degradation. Furthermore, suppressive...
Microtubule-associated protein (MAP) 2 has been perceived as a static cytoskeletal enriched in neuronal dendritic shafts. Emerging evidence indicates dynamic functions for various MAPs activity-dependent synaptic plasticity. However, it is unclear how MAP2 associated with plasticity mechanisms. Here, we demonstrate that specific silencing of high-molecular-weight vivo abolished induction long-term potentiation (LTP) the Schaffer collateral pathway CA1 pyramidal neurons and vitro blocked...
Nitric oxide (NO) plays an important role in anoxic preconditioning to protect the heart against ischemia-reperfusion injuries. The present work was performed study better NO-cGMP-protein kinase G (PKG) signaling pathway activation of both sarcolemmal and mitochondrial ATP-sensitive K+ (KATP) channels during (APC) final influence on reducing anoxia-reperfusion (A/R)-induced cardiac damage rat hearts. upstream regulating elements controlling NO-cGMP-PKG signal-induced KATP channel opening...
ABSTRACT Beta interferon (IFN-β) is involved in a wide range of cellular functions, and its secretion must be tightly controlled to inhibit viral spreading while minimizing damage. Intracellular replication triggers signaling cascades leading the activation transcription factors NF-κB regulatory factor 3 (IRF3) IRF7 (IRF3/7), which synergistically bind IFN-β gene promoter induce expression. The mitochondrial antiviral protein (MAVS) governing adaptor that mediates communications between...
RIG-I is a key cytosolic RNA sensor that mediates innate immune defense against virus. Aberrant activity leads to severe pathological states such as autosomal dominant multi-system disorder, inflammatory myophathies and dermatomyositis. Therefore, identification of regulators ensure efficient without harmful immune-pathology particularly critical deal with RIG-I-associated diseases. Here, we presented the inducible FAT10 novel negative regulator RIG-I-mediated response. In various cell...
Other SectionsABSTRACTINTRODUCTIONCOORDINATED REGULATION OF BIDIRECTIONAL TRANSPORT WITHIN THE GOLGI COMPLEXCARGO SORTING AND IN TRANS-GOLGI NETWORKSIGNALING PATHWAYS REGULATED BY COMPLEX FOR ORGANELLE HOMEOSTASISCONCLUSIONACKNOWLEDGEMENTSCONFLICTS INTERESTFIGURESREFERENCES
We investigated the role of mitochondrial ATP-sensitive K(+) (K(ATP)) channel, big-conductance Ca(2+)-activated (BK(Ca)) and permeability transition pore (MPTP) in ouabain-induced increase Ca(2+) native rat ventricular myocytes by loading cells with rhod 2-AM. To overload Ca(2+), we pretreated ouabain before applying K(ATP) or BK(Ca) channel and/or MPTP opener. Ouabain (1 mM) increased 2-sensitive fluorescence intensity (160 +/- 5.0% control), which was dramatically decreased to control...
Newly synthesized proteins in the endoplasmic reticulum (ER) are sorted by coat protein complex II (COPII) at ER exit site en route to Golgi. Under cellular stresses, COPII become targets of regulation control transport. Here, we show that outer Sec31 and Sec13 selectively sequestered into biomolecular condensate SCOTIN/SHISA-5, which interferes with vesicle formation inhibits ER-to-Golgi SCOTIN is an transmembrane a cytosolic intrinsically disordered region (IDR), required essential for...
Proteoglycans function not only as structural components of the extracellular compartment but also regulators various cellular events, including cell migration, inflammation, and infection. Many microbial pathogens utilize proteoglycans to facilitate adhesion invasion into host cells. Here we report a secreted form novel heparan sulfate proteoglycan that functions against virus The expression SPOCK2/testican-2 was significantly induced in virus-infected lungs or interferon (IFN)-treated...
MON-2, which mediates Golgi-endosome trafficking, mitochondrial inhibition–induced longevity by enhancing autophagy.
Calcineurin-binding protein 1 (CABIN-1) regulates calcineurin phosphatase activity as well the activation, apoptosis, and inflammatory responses of fibroblast-like synoviocytes (FLS), which actively participate in chronic rheumatoid arthritis (RA). However, mechanism action CABIN-1 FLS apoptosis is not clear. This study was undertaken to define regulatory role from mice with collagen-induced (CIA).Transgenic overexpressing human joint tissue under control a type II collagen promoter were...
To determine the functional specificity of inflammation, it is critical to orchestrate timely activation and repression inflammatory responses. Here, we explored PAF1 (RNA polymerase II associated factor)-mediated signal- locus-specific genes induced through pro-inflammatory cytokine interleukin (IL)-1β. Using microarray analysis, identified target whose expression was further enhanced by knockdown in IL-1β–stimulated HepG2 hepatocarcinomas. bound near transcription start sites dissociated...
The PAF complex (PAFc) participates in various steps of the transcriptional process, from initiation to termination, by interacting with and recruiting proteins proper locus for each step. PAFc is an evolutionarily conserved, multi-protein comprising PAF1, CDC73, CTR9, LEO1, yRTF1 and, humans, hSKI8. These components work together, their protein levels are closely interrelated. In present study, we investigated mechanism PAF1 degradation. We found that negatively regulated expression CNOT4,...
ATG2: autophagy related 2; BECN1: beclin 1; COPII: coat protein II; DMSO: dimethyl sulfoxide; EBSS: Earle's balanced salt solution; EGFP: enhanced green fluorescent protein; ER: endoplasmic reticulum; ERES: ER exit site(s); GFP: H89: H-89 dihydrochloride hydrate; LAMP1: lysosomal-associated membrane MAP1LC3/LC3: microtubule-associated 1 light chain 3; MTORC1: mechanistic target of rapamycin kinase complex NS5A: nonstructural 5A; PIK3C3/VPS34: phosphatidylinositol 3-kinase catalytic subunit...
Abstract The ER regulates the spatiotemporal organization of endolysosomal systems by membrane contact. In addition to tethering via heterotypic interactions on both organelles, we present a novel ER‐endosome mechanism mediated homotypic interactions. single‐pass transmembrane protein SCOTIN is detected in and endosomes. ‐knockout (KO) cells, ER‐late endosome contacts are reduced, perinuclear positioning endosomes disturbed. cytosolic proline‐rich domain (PRD) forms assemblies vitro...
A major goal of breast cancer research is to prevent the molecular events that lead tumour metastasis. It well-established both cytoplasmic and mitochondrial reactive oxygen species (ROS) play important roles in cell migration Accordingly, this study examined mechanisms anti-metastatic effects NecroX-5, a ROS scavenger. NecroX-5 inhibited lung metastasis by ameliorating mouse model. In human cells, inhibition type-dependent. We observed effect correlated with reduction ROS, but MitoQ did not...
The posterior medial nucleus of the thalamus (POm) and vibrissal primary motor cortex (vM1) convey essential information to barrel (S1BF) regarding whisker position movement. Therefore, understanding relative spatial relationship these two inputs is a critical prerequisite for acquiring insights into how S1BF synthesizes interpret location an object. Using array tomography, we identified locations synapses from vM1 POm on distal tuft dendrites L5 pyramidal neurons where are combined....
The hair growth cycle consists of the anagen, catagen, and telogen phases, follicle dermal papilla (HDP) cells human play a role in initiation maintenance anagen phase. Reduction HDP contributes to loss; however, limited treatment options are associated with negative side effects. Therefore, naturally derived substance loss-preventing properties is needed.We investigated growth-stimulating activities Plantago asiatica L. extract (PAE) its molecular mechanism cells.Cell proliferation was...