A5075392155- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Systemic Lupus Erythematosus Research
- Cancer, Lipids, and Metabolism
- Antioxidant Activity and Oxidative Stress
- Endometriosis Research and Treatment
- Blood Coagulation and Thrombosis Mechanisms
- Celiac Disease Research and Management
- Cell Adhesion Molecules Research
- Pregnancy and Medication Impact
- Fatty Acid Research and Health
- Birth, Development, and Health
- Preterm Birth and Chorioamnionitis
- Testicular diseases and treatments
- Free Radicals and Antioxidants
- Neonatal Respiratory Health Research
- Retinoids in leukemia and cellular processes
- Adipokines, Inflammation, and Metabolic Diseases
- Gastrointestinal disorders and treatments
- Pregnancy-related medical research
- Intestinal and Peritoneal Adhesions
- Cholesterol and Lipid Metabolism
- Hedgehog Signaling Pathway Studies
- Immune Response and Inflammation
- Pituitary Gland Disorders and Treatments
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2025
Università Cattolica del Sacro Cuore
2013-2025
Agostino Gemelli University Polyclinic
2016-2023
University of the Sacred Heart
2007
University of Calabria
2004
University of Rome Tor Vergata
1999
n-3 Polyunsaturated fatty acids (PUFAs) inhibit the development of microvessels in mammary tumors growing mice. Human colorectal produce vascular endothelial growth factor (VEGF) whose expression is up-regulated tumor cells by both cyclooxygenase-2 (COX-2) and PGE(2) directly correlated to neoangiogenesis clinical outcome. The goal this study was examine capability PUFAs regulate VEGF HT-29 human vitro vivo. Constitutive augmented cultured serum starvation effects eicosapentaenoic (EPA) or...
Although an antioxidant mechanism has been involved in the beneficial effects of ferulic acid human diseases, there are few reports on properties this compound isolated membranes and intact cells. Here, we evaluated ability inhibiting lipid peroxidation rat liver microsomal reactive oxygen species production NIH-3T3 fibroblasts, induced by both tert-BOOH AAPH. We also compared its efficiency with that other antioxidants, such as alpha-tocopherol, beta-carotene, ascorbic acid, added alone or...
Antiphospholipid antibodies (aPL) represent an important risk factor for thrombosis and recurrent miscarriage in patients with antiphospholipid syndrome (APS). The mechanisms of aPL-mediated pregnancy failure have been researched. Previous studies demonstrated that aPL bind trophoblast cells, reducing proliferation, human chorionic gonadotrophin release, vitro invasiveness. Recent data suggest are also able to react decidual inducing a proinflammatory phenotype. Decidua, newly formed tissue...
Preeclampsia (PE) is a major cause of maternal and neonatal morbidity mortality. Epidemiological association between Helicobacter pylori (Hp) infection PE onset has been widely shown. The aim this study was to analyze possible correlation Hp the severity clinical presentation identify an immunologic mechanism triggered by potentially contributing pathogenesis PE.Sera from 93 preeclamptic women 87 healthy pregnant were tested for immunoassay anti-CagA antibodies Western blot assay. serologic...
Although the pharmacological role of β-carotene in prevention and treatment colon cancer has received increasing attention, little is known about molecular mechanisms action this carotenoid. The present study demonstrates that β-carotene, a natural pigment widely fruit vegetables, inhibits growth several human adenocarcinoma cell lines (COLO 320 HSR, LS-174, HT-29 WiDr) by inducing cycle arrest G 2 /M phase apoptosis. These effects were dose time dependent strictly related to ability...
Non-steroidal anti-inflammatory drugs cause enterocyte damage inducing an increase of intestinal permeability. Tight junctions are the key structures in permeability mucosa. ZO-1 is a tight junction associated protein considered good marker their integrity. It has been suggested that probiotics could play protective role barrier function. We determined, vitro, whether heat-killed Lactobacillus acidophilus strain LB (LaLB) with its spent culture supernatant protects HT-29 cells from aspirin...
Abstract Background Previous studies reported an epidemiological association between C ag A ‐positive H . pylori strains and pre‐eclampsia. As antibodies anti‐ cross‐react with endothelial cells trophoblast show phenotypic profile, we hypothesized that may recognize antigens of cytotrophoblast cells, thus impairing their function. Materials Methods Placenta samples were obtained from healthy women. Cytotrophoblast cultured in a medium containing increasing concentration polyclonal...
Recurrent pregnancy loss (RPL) occurs in 3–5% about 30% of cases no cause can be found. Women with RPL show higher prevalence undiagnosed gut disorders. Furthermore, endometrial tissues women, expression pro-inflammatory cytokines and Nalp-3 inflammasome has been observed. Aim this study was to investigate whether an abnormal permeability might occur women allow passage into systemic circulation molecules able induce inflammation. 70 idiopathic 30 healthy were recruited at the Pregnancy Loss...
Endometriosis is an estrogen-linked gynecological disease defined by the presence of endometrial tissue on extrauterine sites where it forms invasive lesions. Alterations in estrogen-mediated cellular signaling seems to have essential role pathogenesis endometriosis. Higher estrogen receptor (ER)-β levels and enhanced ER-β activity were detected endometriotic tissues. It well known that interacts with components cytoplasmic inflammasome-3 (NALP-3), NALP-3 activation increases interleukin...
Resistin is a novel hormone that secreted by human adipocytes and mononuclear cells probably associated with insulin resistance. Recently, resistin has been postulated to play role in pregnancy, gene expression observed placental tissues. However, it still not known if able affect trophoblast functions development. Therefore, we investigated the hypothesis might regulate production of matrix metalloproteinases (MMPs), tissue inhibitors (TIMPs), invasive behavior angiogenic processes. In...
The association between maternal celiac disease (CD) and both reduced fertility increased risk of adverse pregnancy-related events has been long documented. However, no evidences are available regarding the pathogenic mechanisms this link. aim study was to determine whether anti-tissue transglutaminase (anti-tTG) antibodies involved in damage trophoblastic cells vitro.Human primary cells, isolated from term placenta, were exposed anti-tTG immunoglobulin G (IgG) antibodies, commercially...
Heparin-binding epidermal growth factor-like factor (HB-EGF) plays a role in blastocyst implantation and is down-regulated preeclampsia hypertensive pregnancy disorders associated with defective extravillous trophoblast invasion. Defective placentation severe are also features of the antiphospholipid syndrome (APS). The purpose this study was to investigate whether abnormal HB-EGF expression pathogenic antibody (aPL)-mediated placentation.HB-EGF placental tissue evaluated by Western blotting...
Antiphospholipid syndrome (APS) is an autoimmune disorder characterized by vascular thrombosis and/or pregnancy morbidity in the presence of circulating antiphospholipid antibodies (aPL). Different pathogenic mechanisms for aPL-mediated failure have been proposed. In particular a direct effect aPL on both maternal and fetal side placental tissue has reported, since their reactivity with β2-glycoprotein I (β2GPI) makes them adhere to trophoblast human endometrial endothelial cell (HEEC)...
Problem Aim of our study was to investigate whether TIFI, a syntetic peptide able compete with anti‐phospholipid antibodies (aPL) in the binding endothelium, may restore aPL‐inhibited endometrial angiogenesis. Methods The protective role TIFI evaluated on: i) aPL–inhibited human endothelial cells (HEEC) angiogenesis vitro ; ii) vascular growth factor (VEGF) and metalloproteases (MMPs) expression; iii) Nuclear Factor‐κB (NF‐κB) Extracellular signal–Regulated Kinase (ERK) activation (iv) vivo...
Celiac disease (CD) is an autoimmune enteropathy triggered by gluten ingestion and characterized circulating anti-transglutaminase type 2 (anti-TG2) autoantibodies. An epidemiological link between maternal CD increased risk of pregnancy failure has been established; however, the mechanism underlying this association still poorly understood. Because proper endometrial angiogenesis decidualization are prerequisites for placental development, we investigated effect anti-TG2 antibodies on...
Problem A threefold higher prevalence of antinuclear antibodies (ANA) has been reported in patients with recurrent pregnancy loss (RPL). Nevertheless, the role ANA reproductive failure is still unclear. The aim this study was to investigate during early vivo. Method We used pregnant mice treated immunoglobulin G (IgG) obtained from normal healthy subjects (NHS); ANA+ sera RPL; and women uncomplicated pregnancies (HW). Placental immunohistochemical/immunofluorescence staining performed detect...
Granulosa cell (GC) differentiation, stimulated by FSH and LH, drives oocyte maturation follicle development. promotes GC proliferation, LH triggers ovulation. In clinical practice, hCG is used to mimic LH. Despite various controlled ovarian stimulation (COS) protocols employing exogenous gonadotropins GnRH analogs prevent premature ovulation, their effectiveness safety remain debated. To identify markers predicting a positive treatment response, the secretome of gonadotropin-stimulated...