A5035465038- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immune responses and vaccinations
- vaccines and immunoinformatics approaches
- Long-Term Effects of COVID-19
- COVID-19 Impact on Reproduction
- Immunotherapy and Immune Responses
Benaroya Research Institute
2021-2024
Virginia Mason Medical Center
2022-2024
The aim of this study was to define the breadth and specificity dominant SARS-CoV-2-specific T cell epitopes using a comprehensive set 135 overlapping 15-mer peptides covering SARS-CoV-2 envelope (E), membrane (M) nucleoprotein (N) in cohort 34 individuals with acute (n = 10) resolved 24) COVID-19. Following short-term virus-specific vitro cultivation, single peptide-specific CD4+ response each patient screened enzyme linked immuno spot assay (ELISpot) confirmed by single-peptide...
Class II tetramer reagents for eleven common DR alleles and a DP allele prevalent in the world population were used to identify SARS-CoV-2 CD4+ T cell epitopes. A total of 112, 28 42 epitopes specific Spike, Membrane Nucleocapsid, respectively, with defined HLA-restriction identified. Direct ex vivo staining PBMC was define immunodominant subdominant estimate frequencies these cells exposed naïve individuals. Majority identified have <67% amino acid sequence identity endemic coronaviruses...
Here, we longitudinally assessed the ex vivo frequency and phenotype of SARS-CoV-2 membrane protein (aa145-164) epitope-specific CD4+ T-cells an anti-CD20-treated patient with prolonged viral positivity in direct comparison to immunocompetent through MHC class II DRB1*11:01 Tetramer analysis. We detected a high stable membrane-specific T-cell response both patients, higher frequencies virus-specific B-cell-depleted patient. However, found altered memory that was skewed towards late...
Class II tetramer reagents were used to identify SARS-CoV-2-specific CD4+ T cell epitopes and study these epitope-specific cells ex vivo. Dominant subdominant identified. Majority of identified have <67% amino acid sequence identity with endemic coronaviruses are unlikely elicit cross-reactive responses. However, four Spike highly homologous Specific for three such DR-restricted present at relatively low frequencies in convalescent individuals. memory that recognized a DPB1*04:01 restricted...