Background and Aim: Chronic Opisthorchis viverrini (OV) infection induces significant biliary changes is a major risk factor for cholangiocarcinoma. However, the role of goblet cell metaplasia (GCM) mucin dynamics in host defense parasite persistence remains poorly understood. This study aims to characterize histological changes, particularly types, compare responses between susceptible (hamsters) non-susceptible (mice) hosts during early chronic OV infection. Materials Methods: Thirty-five...

The Bambara groundnut is the indigenous legume in southern part of Thailand. It contains a seed coat rich polyphenols, which can serve as natural antimicrobial agents. extracts from red and white coats groundnuts, namely RSC WSC, respectively, were prepared using an ultrasound-assisted extraction process. yield, total phenolic content (TPC), activity both examined. extract demonstrated significantly higher yield (8.35%) than WSC (2.34%) (

Summary Rapid softening of texture is a major problem associated with quality loss and consumer rejection harpiosquillid mantis shrimp ( Harpiosquilla raphidea ) (HMS). Effects endogenous proteases in muscles digestive tract on protein degradation were investigated. Scanning electron microscopic histological images indicated drastic destruction muscle during iced storage. Based autolysis, maximal activity was found at pH 8.5 55 °C, serine including trypsin dominant. Cathepsin B L + altered...

Abstract Coccidiosis is an important disease in juvenile fish because of severe intestinal injury during infection. We first reported the mixed infection coccidia and its association with health status pathological findings Asian seabass ( Lates calcarifer ) cultured Thailand. Two groups seabass, 60‐day 90‐day fish, were sampled to investigate prevalence coccidian intensity using morphological characterization PCR. Phylogenetic analysis 18S rRNA gene amplified from intestines revealed...

Abstract Objective Acute mortality with clinical symptoms of streptococcal-like infections was observed in red tilapia Oreochromis sp. cultured floating cages Prachin Buri Province, Thailand, during May 2023. Herein, we identified an emerging pathogen, Lactococcus garvieae, as the etiological agent. Methods After bacterial isolation from brain and kidney diseased fish, identification performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry VITEK 2 system....

Co-infection with the cagA strain of Helicobacter pylori exacerbates pathology human liver fluke Opisthorchis viverrini (OV) infection leading to cholangiocarcinoma. However, underlying mechanisms remain unclear. We report a significant increase in cagA-positive and cagA-negative H. stomach, blood, bile, OV worms co-infected Syrian golden hamsters at one hour, three hours, month, post-infection, compared infected either or alone. Except worms, numbers declined months particularly bile fluid...

Abstract Opisthorchis viverrini is a carcinogenic parasite that can cause bile duct cancer called cholangiocarcinoma. A study of the immune response this in susceptible and non-susceptible hosts may provide clue to develop vaccines immunodiagnostic markers, which are currently not available. Here, we compared antibody Golden Syrian hamsters BALB/c mice infected by liver fluke. In mice, was detected between 1 2 weeks post-infection, whereas it positive 4 post-infection hamsters....

Bio-calcium derived from fish frames may offer several advantages for osteoporosis prevention. This study aimed to evaluate the effects of bio-calcium skipjack tuna on bone loss in ovariectomized rats. Tuna was prepared through enzymatic hydrolysis, defatting, bleaching, and grinding processes. The bioavailability calcium tested using Caco-2 cell monolayer model, showing that 13% absorbed, compared 10% carbonate. Rats were divided into five following groups: (1) OVX, (2) sham-operated, (3),...

Background: Individual host susceptibility is believed to be a risk factor in the interaction between and parasite. Since studying time series humans limited, animal models are replaced. Aim: This study aims explore compare pattern of inflammatory cell types along biliary tract their association with proliferative lesions early development CCA from susceptible non-susceptible models. Methods: Thirty male Syrian golden hamsters 30 BALB/c mice, serving as models, were used this comparative...

Background and Aim: Coccidian infection (coccidiosis) is one of the most important causes illness death in fish population, including Asian sea bass. The fingerling developmental stage sensitive to various infectious agents. Economic losses are sustained by bass aquaculture industry due coccidiosis annually. However, related pathological changes fingerlings' three-part intestine remain unknown. This study aimed investigate rate, location site, specific lesions small intestinal tissues a...

Opisthorchis viverrini infection-induced inflammation contributes to cholangiocarcinoma (CCA) development in humans and animals. Inflammation generates free radicals, such as reactive oxygen species nitrogen (RNS), which damage the host's DNA. However, only 5% of O. viverrini-infected individuals develop malignancy, suggesting that variations inflammatory response parasite may influence susceptibility. Due limitations studying human susceptibility, we used an animal model investigate...

Harpiosquillid mantis shrimp (

Inflammation caused by

Abstract Piscine intestinal Cryptosporidium infection is a known cause of severe illness and mortality in juvenile Asian sea bass, with inflammation playing central role. This triggers the generation reactive oxygen species (ROS), leading to irreversible DNA damage activation apoptosis pathways. However, specific impact on ROS production, damage, this context remains unclear. study investigated pathogenic role causing cell piscine cryptosporidiosis. Forty‐four samples from 60‐ 90‐day bass...

Cetaceans and sirenians are endangered marine mammals that threatened by stranding mortality. In Thailand's Andaman Sea, limited data exist on the causes patterns of these events. This retrospective study investigated characterization underlying cetacean sirenian mortality events in Sea from 2018 to 2023 using information Department Marine Coastal Resources, Thailand. Data 363 stranded mammals, including both live dead strandings, species types, carcass condition, necropsy findings...

Roundworms cause infections in the avian population that lead to illness and poor production. The singing zebra dove is an economically important animal Indo-Malay region. prevalence of these parasitic groups doves unknown. This study estimated associated human risk factors gastrointestinal nematode farming. A cross-sectional survey was conducted from January April 2021. on 184 three farms. Fecal samples were collected pooled droppings. Major proportions infection intensity nematodes...

Prolonged assisted ventilation during anesthesia and critical care is associated with increased morbidity mortality in both humans animals. However, no study of mechanically ventilated patients has examined the mortality-related physiology pathology. Our investigated physiological, hematological pathological changes pigs that died anesthesia. Six were placed under ventilation-assisted for up to 48h. The heart rate (HR), respiratory (RR), mean arterial blood (MAP), end-tidal carbon dioxide...