A5040517947- Nerve injury and regeneration
- Pharmacological Effects and Toxicity Studies
- Neurogenesis and neuroplasticity mechanisms
- Obsessive-Compulsive Spectrum Disorders
- Stress Responses and Cortisol
- Electroconvulsive Therapy Studies
- Epilepsy research and treatment
- Treatment of Major Depression
- Diet and metabolism studies
- Cancer Treatment and Pharmacology
- Schizophrenia research and treatment
- Tryptophan and brain disorders
- Cytokine Signaling Pathways and Interactions
- Neuroblastoma Research and Treatments
- Bipolar Disorder and Treatment
- Drug-Induced Ocular Toxicity
- Anesthesia and Sedative Agents
- Autism Spectrum Disorder Research
- Neurological and metabolic disorders
- Poisoning and overdose treatments
- Parkinson's Disease and Spinal Disorders
- Photoreceptor and optogenetics research
- Analytical Chemistry and Chromatography
- Blood disorders and treatments
- Hematological disorders and diagnostics
A.T. Still University
2012-2023
University of St Andrews
2010
University of Southampton
2010
University of Nebraska Medical Center
2006-2007
St. Jude Children's Research Hospital
1998-2000
Salk Institute for Biological Studies
1988-1994
University of California, San Diego
1992
Karolinska Institutet
1992
California Institute of Technology
1983-1985
University of Utah
1985
We have screened an adult rat cerebellar cDNA library in search of novel protein tyrosine-kinase (PTK) cDNAs. A for a putative PTK, trkB, was cloned, and its sequence indicates that it is likely to be derived from gene ligand-regulated receptor closely related the human trk oncogene. Northern (RNA) analysis showed trkB expressed predominantly brain expresses multiple mRNAs, ranging 0.7 9 kb. Hybridization cerebral mRNAs with variety probes there are encoding truncated receptors. Two...
ABSTRACT The human immunodeficiency virus type 1 (HIV-1) protease inhibitors (PIs)—saquinavir, ritonavir, nelfinavir, and indinavir—interact with the ABC-type multidrug transporter proteins MDR1 MRP1 in CEM T-lymphocytic cell lines. Calcein fluorescence was significantly enhanced + CEM/VBL100 CEM/VM-1-5 cells incubated presence of various HIV PIs calcein acetoxymethyl ester. also cytotoxic activity doxorubicin, a known substrate for MRP1, both VBL100 VM-1-5 Saquinavir, nelfinavir doxorubicin...
Expression of neurotrophins and neurotrophin receptors was examined with in situ hybridization immunohistochemical techniques 10 days to 6 weeks after ventral or dorsal funiculus spinal cord lesions adult rats cats, that have previously been shown allow axon regrowth. Strongly elevated levels trkB mRNA were seen the scar tissue formed white matter both types lesions. Only small increases detected for nerve growth factor, brain-derived neurotrophic 3, 4, trk, trkC response injuries....
We have screened an adult rat cerebellar cDNA library in search of novel protein tyrosine-kinase (PTK) cDNAs. A for a putative PTK, trkB, was cloned, and its sequence indicates that it is likely to be derived from gene ligand-regulated receptor closely related the human trk oncogene. Northern (RNA) analysis showed trkB expressed predominantly brain expresses multiple mRNAs, ranging 0.7 9 kb. Hybridization cerebral mRNAs with variety probes there are encoding truncated receptors. Two...
The TrkB receptor protein-tyrosine kinase is a for brain-derived neurotrophic factor and neurotrophin-3. In response to neurotrophin-3 treatment, expressed exogenously in Rat-2 cells rapidly phosphorylated on tyrosine residues. At least 2 regions of contain tyrosines. major sites autophosphorylation are the region containing Tyr-670, Tyr-674, Tyr-675, which lies domain corresponds by sequence homology Tyr-416 site p60c-Src. Tyr-785, just COOH-terminal side relatively short tail...
Brain-derived neurotrophic factor (BDNF) promotes neuronal survival and protection against damage. We addressed whether BDNF might promote chemoprotection in neuroblastoma (NB) using a drug-sensitive human NB cell line. All-trans-retinoic acid (ATRA) induces striking phenotypic differentiation of NB1643 cells, exogenous treatment these differentiated cells. ATRA TRKB expression, stimulates both autophosphorylation induction the immediate early gene, FOS, cells.BDNF mRNA is expressed Because...
Brain-derived neurotrophic factor (BDNF) and other neurotrophins induce a unique prolonged activation of mitogen-activated protein kinase (MAPK) compared with growth factors. Characterization kinetic spatial modeling the signaling pathways underlying this MAPK by BDNF will be important in understanding physiological role many complex systems nervous system. In addition to Shc, fibroblast receptor substrate 2 (FRS2) is required for BDNF-induced MAPK. induces phosphorylation FRS2. However,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIdentification of subunits acetylcholine receptor that interact with a cholesterol photoaffinity probeDavid S. Middlemas and Michael A. RafteryCite this: Biochemistry 1987, 26, 5, 1219–1223Publication Date (Print):March 1, 1987Publication History Published online1 May 2002Published inissue 1 March 1987https://pubs.acs.org/doi/10.1021/bi00379a003https://doi.org/10.1021/bi00379a003research-articleACS PublicationsRequest reuse permissionsArticle...
The acetylcholine receptor (AChR) from Torpedo electroplax can be isolated in a pure form either its native membrane-bound state or by affinity chromatography after solubilization. This is due to very dense packing of the AChR molecules postsynaptic membrane that excludes other intrinsic components. Purified fragments reseal, forming closed, right-side-out vesicles, which used for functional and structural studies AChR.
Major depressive disorder (MDD) is arguably the largest contributor to global disease and disability burden, but very few treatment options exist for juvenile MDD patients. Ghrelin principal hunger-stimulating peptide, it has also been shown reduce depressive-like symptoms in adult rodents. We examined effects of intracerebroventricular (icv) injection ghrelin on behavior. Moreover, we determined whether increased neurogenesis hippocampus. (0.2-nM, 0.5-nM, 1.0-nM) was administered acutely by...
Brain-derived neurotrophic factor (BDNF) promotes neuronal survival. Gaining an understanding of how BDNF, via the tropomyosin-related kinase B (TRKB) receptor, elicits specific cellular responses is contemporary interest. Expression mutant TrkB in fibroblasts, where tyrosine 484 was changed to phenylalanine, abrogated Shc association with TrkB, but only attenuated and did not block BDNF-induced phosphorylation mitogen-activated protein (MAPK). This suggests there another signaling mechanism...
There has been growing recognition that major depressive disorder is a serious medical also affects children. This accompanied by an increased use of antidepressant drugs in adolescents; however, not all classes antidepressants are effective children and adolescents. increasing need to understand the differences action different developmental stages. some data indicating behavioral effect chronic treatment adult rodents dependent on hippocampal neurogenesis; it known which induce...