A5004590763- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Glycosylation and Glycoproteins Research
- HIV/AIDS Research and Interventions
- Orthopedic Infections and Treatments
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Infectious Diseases and Tuberculosis
- Mosquito-borne diseases and control
- Monoclonal and Polyclonal Antibodies Research
- Spinal Fractures and Fixation Techniques
- COVID-19 epidemiological studies
Tsinghua University
2022-2023
Peking Union Medical College Hospital
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
University of Pittsburgh
2019-2021
The HIV-1 Nef accessory factor is critical to the viral life cycle in vivo and promotes immune escape of infected cells via downregulation cell-surface MHC-I. Previously, we discovered small molecules that bind directly block many its functions, including enhancement infectivity replication T cell lines. These compounds also restore MHC-I expression HIV-infected CD4 from AIDS patients, enabling recognition killing by autologous cytotoxic lymphocytes (CTLs). In this study, describe synthesis...
The HIV-1 virulence factor Nef promotes high-titer viral replication, immune escape, and pathogenicity. interacts with interleukin-2-inducible T-cell kinase (Itk) Bruton's tyrosine (Btk), two Tec-family kinases expressed in target cells (CD4 T macrophages, respectively). Using a cell-based bimolecular fluorescence complementation assay, here we demonstrate that recruits both Itk Btk to the cell membrane induces constitutive activation transfected 293T cells. homodimerization-defective...
Non-receptor protein-tyrosine kinases regulate cellular responses to many external signals and are important drug discovery targets for cancer infectious diseases. While assays exist the assessment of kinase activity in vitro, methods that report changes tyrosine single cells have potential provide information about at cell population level. In this protocol, we combined bimolecular fluorescence complementation (BiFC), an established method protein-protein interactions, immunofluorescence...
Abstract Background Patients with suspected tuberculosis of the spine often present back pain and/or neurological symptoms due to underlying compression fractures. Needle biopsy is frequently needed for confirmatory diagnosis. An additional surgical procedure may be required stabilize and prevent complications. Methods T0 retrospectively reviewed medical records 44 patients spinal from 2013–2017 who underwent bone diagnosis simultaneous PVP stabilization spine. A follow-up telephone...