A5102761865- Viral Infections and Vectors
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Plant and Fungal Interactions Research
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Immunodeficiency and Autoimmune Disorders
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Gastrointestinal disorders and treatments
- HIV Research and Treatment
- Plant Virus Research Studies
- Parvovirus B19 Infection Studies
- Animal Disease Management and Epidemiology
- Bartonella species infections research
- Hepatitis B Virus Studies
- Diabetes and associated disorders
Columbia University
2018-2023
We employed high throughput sequencing to survey the microbiomes of Ixodes scapularis collected in New York and Connecticut. examined 197 individual I. adults pools from 132 nymphs. detected Borrelia burgdorferi sensu stricto 56.3% ticks, Anaplasma phagocytophilum 10.6%, miyamotoi 5%, Babesia microti 7.6%, Powassan virus 3.6%. did not detect mayonii, Ehrlichia muris eauclairensis, Bartonella spp. or pathogenic species other than B. microti. The most abundant bacterium (65%), only rickettsial...
Abstract Ticks are vectors of a wide variety pathogens that implicated in mild to severe disease humans and other animals. Nonetheless, the full range tick-borne is unknown. Viruses, particular, have been neglected discovery efforts targeting agents. High throughput sequencing was used characterize virome 638 ticks, including Rhipicephalus microplus (n = 320), sanguineus 300), Amblyomma ovale 18) collected throughout Trinidad Tobago 2017 2018. Sequences representing nine viruses were...
Tick-borne diseases are a serious threat to both public and veterinary health. In this study, we used high-throughput sequencing characterize the virome of three tick species implicated in spread vector-borne disease throughout Croatia. Ten viruses were identified, including seven potential novel within viral families Flaviviridae, Nyamiviridae, Rhabdoviridae, Peribunyaviridae, Phenuiviridae, Nairoviridae.
Background: Polymerase chain reaction analyses of cardiac tissues have detected viral sequences in up to 67% cases myocarditis. However, viruses not been implicated giant cell myocarditis (GCM). Furthermore, efforts detect unsuccessful more accessible samples such as peripheral blood. Methods: We used Virome Capture Sequencing for Vertbrate Viruses (VirCapSeq-VERT), a method that simultaneously screens all known vertebrate viruses, investigate 33 patients with investigated blood mononuclear...
Recent advances in high-throughput sequencing technology have led to a rapid expansion the number of viral sequences associated with samples from vertebrates, invertebrates and environmental samples. Accurate host identification can be difficult assays complex that contain more than one potential host. Using unbiased metagenomic sequencing, we investigated wild house mice (Mus musculus) brown rats (Rattus norvegicus) New York City determine aetiology liver disease. Light microscopy was used...
Abstract Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is estimated to affect 0.4%–2.5% of the global population. Most cases are unexplained; however, some patients describe an antecedent viral infection or response antiviral medications. We report here a multicenter study for presence nucleic acid in blood, feces, and saliva with ME/CFS using polymerase chain reaction high‐throughput sequencing. found no consistent group‐specific differences other than lower prevalence...
Abstract Background Viral infections have been suggested as possible triggers for the onset of ulcerative colitis (UC). Methods We employed VirCapSeq-Vert, a high-throughput sequencing virus capture platform, to examine stool virome children with newly diagnosed moderate severe UC. surveyed fecal samples collected at presentation, after symptom remission, and from control group irritable bowel syndrome. Results Seventy subjects UC (mean age 13 years, 45 had symptoms, 25 severe, 69 70 Mayo...
Hunters are at a higher risk for exposure to zoonotic pathogens due their close interactions with wildlife and arthropod vectors. In this study, high throughput sequencing was used explore the viromes of two tick species, Amblyomma dissimile Haemaphysalis juxtakochi, removed from hunted in Trinidad Tobago. We identified sequences 3 new viral families Orthomyxoviridae, Chuviridae Tetraviridae A. dissimile.
New technologies enable viral discovery in a diversity of hosts, providing insights into evolution. We used one such approach, the virome capture sequencing for vertebrate viruses (VirCapSeq-VERT) platform, on 21 samples originating from six dead Maxwell's duikers (Philantomba maxwellii) Taï National Park, Côte d'Ivoire. detected presence an orthohepadnavirus animal and characterized its 3128 bp genome. The highest copy numbers were spleen, followed by lung, blood, liver, with lowest kidney...