A5022772823- HIV Research and Treatment
- Nanoparticles: synthesis and applications
- HIV/AIDS Research and Interventions
- Antifungal resistance and susceptibility
- Nanocluster Synthesis and Applications
- Bacterial biofilms and quorum sensing
- Cytomegalovirus and herpesvirus research
- Antimicrobial agents and applications
- COVID-19 Impact on Reproduction
- Environmental remediation with nanomaterials
- Nanomaterials for catalytic reactions
- Aquaculture disease management and microbiota
- Advanced Nanomaterials in Catalysis
- Reproductive System and Pregnancy
- HIV-related health complications and treatments
- HIV, Drug Use, Sexual Risk
- Medical and Biological Ozone Research
- Reproductive tract infections research
- Immune Cell Function and Interaction
- Food Safety and Hygiene
- Selenium in Biological Systems
- Trace Elements in Health
- Parasite Biology and Host Interactions
- Herpesvirus Infections and Treatments
- HIV/AIDS drug development and treatment
Texas Center for Infectious Disease
2018-2023
The University of Texas at San Antonio
2015-2023
University of Monterrey
2014-2015
Winston-Salem State University
2011-2012
Universidad Autónoma de Nuevo León
2005-2011
Cupron (United States)
2007
Kaplan Medical Center
2004-2007
Weizmann Institute of Science
2003
Hebrew University of Jerusalem
2003
The interaction of nanoparticles with biomolecules and microorganisms is an expanding field research. Within this field, area that has been largely unexplored the metal viruses. In work, we demonstrate silver undergo a size-dependent HIV-1, exclusively in range 1-10 nm attached to virus. regular spatial arrangement nanoparticles, center-to-center distance between fact exposed sulfur-bearing residues glycoprotein knobs would be attractive sites for nanoparticle suggest interact HIV-1 virus...
Silver nanoparticles have proven to exert antiviral activity against HIV-1 at non-cytotoxic concentrations, but the mechanism underlying their HIV-inhibitory has not been fully elucidated. In this study, silver are evaluated elucidate mode of action using a panel different in vitro assays. Our data suggest that anti-HIV an early stage viral replication, most likely as virucidal agent or inhibitor entry. bind gp120 manner prevents CD4-dependent virion binding, fusion, and infectivity, acting...
Candida albicans is the most common pathogenic fungus isolated in bloodstream infections hospitalized patients, and candidiasis represents fourth infection United States hospitals, mostly due to increasing numbers of immune- medically-compromised patients. C. has ability form biofilms morphogenetic conversions between yeast hyphal morphologies contribute biofilm development represent an essential virulence factor. Moreover, these attached communities cells are surrounded by a protective...
Silver nanoparticles, which are being used increasingly as antimicrobial agents, may extend its antibacterial application to methicillin-resistant Staphylococcus aureus (MRSA), the main cause of nosocomial infections worldwide. To explore properties silver nanoparticles against MRSA, present work includes an analysis relation between nanosilver effect and MRSA's resistance mechanisms, a study size dependence bactericidal activity toxicity assessment epithelial human cells. Minimum inhibitory...
Previous in vitro studies have demonstrated that polyvinylpyrrolidone coated silver nanoparticles (PVP-coated AgNPs) antiviral activity against HIV-1 at non-cytotoxic concentrations. These particles also demonstrate broad spectrum virucidal by preventing the interaction of gp120 and cellular CD4, thereby inhibiting fusion or entry virus into host cell. In this study, we evaluated PVP-coated AgNPs as a potential topical vaginal microbicide to prevent transmission infection using human...
Candida auris is an emerging pathogenic fungus implicated in healthcare-associated outbreaks and causes bloodstream infections associated with high mortality rates. Biofilm formation represents one of the major pathogenetic traits this microorganism. Unlike most other species, C. has ability to survive for weeks on different surfaces. Therefore, there urgent need develop new effective control strategies combat threat auris. Advances nanotechnologies have emerged that carry significant...
Candida albicans is a major opportunistic fungal pathogen. One of the most important virulence factors that contribute to pathogenesis candidiasis its ability form biofilms. A key characteristic biofilms their resistance antifungal agents. Due significant morbidity and mortality rates related biofilm-associated drug resistance, there an urgency develop novel nanotechnology-based approaches preventing biofilm-related infections.In this study, we report, for first time, synthesis selenium...
ABSTRACT Human immunodeficiency virus type 1 (HIV-1) can be transmitted through breast-feeding and contaminated blood donations. Copper has potent biocidal properties been found to inactivate HIV-1 infectivity. The objective of this study was determine the capacity copper-based filters in culture media. Medium spiked with high titers exposed copper oxide powder or oxide-impregnated fibers passed filters, infectious viral before after treatment were determined. Cell-free cell-associated...
We report the capacity of copper oxide-containing filters to reduce infectious titers a panel viruses spiked into culture media. Enveloped, nonenveloped, RNA, and DNA were affected, suggesting possibility using devices deactivate wide spectrum found in filterable suspensions.
HIV/AIDS pandemic is a worldwide public health issue. There need for new approaches to develop antiviral compounds or other therapeutic strategies limit viral transmission. The envelope glycoproteins gp120 and gp41 of HIV are the main targets both silver nanoparticles (AgNPs) neutralizing antibodies. an urgency optimize efficiency antibodies (NABs). In this study, we demonstrated that there additive effect between four NABs AgNPs when combined against cell-associated HIV-1 infection in...
Silver nanoparticles offer a possible means of fighting antibacterial resistance. Most their properties are attributed to silver ions. In the present work, we study actions positively charged against both methicillin-sensitive Staphylococcus aureus and methicillin-resistant . We use aberration-corrected transmission electron microscopy examine bactericidal effects ultrastructural changes in bacteria that induced by nanoparticles. The revealed our 1 nm average size thinning permeabilization...
Infections caused by pathogenic fungi, especially Candida albicans, are a growing global challenge for public health. Current antifungal drugs have been administered commonly to treat infections, yet their high toxicity and the development of multidrug-resistant strains pose threat. The present report describes synthesis, characterization, activity novel class water-soluble conjugated polymers (CPs) bearing imidazolium functionality in polymer backbone that combine both photodynamic...
Objective: To determine the efficacy of UC781 in preventing HIV-1 transmission through cervical tissue. Design: Use human tissue organ culture, which cervix is upper chamber a transwell and infective virus quantified lower chamber. Methods: Five-millimeter pieces tissues are exposed to UC781. After thorough removal drug, high doses cell-free or cell-associated HIV-1. Transmission measured by determining infection target cells Results: Exposure 0.5 μM for 30 min, followed extensive washing...
Here we report on the identification and applications of an aqueous 29-atom silver cluster stabilized with 12 lipoate ligands, i.e. Ag29(R-α–LA)12 or (29,12), wherein R-α–LA = R-α-lipoic acid, a natural dithiolate. Its uniformity is checked by HPLC-ESI-MS analytical ultracentrifugation, which confirms its small dimension (∼3 nm hydrodynamic diameter). For first time, this has been detected intact via electrospray ionization mass spectrometry, allowing one to confirm composition, [3-]...
Both bacterial and fungal organisms display the ability to form biofilms; however, mixed bacterial/fungal biofilms are particularly difficult control eradicate. The opportunistic microbial pathogens Candida albicans Staphylococcus aureus among most frequent causative agents of healthcare-acquired infections, often co-isolated forming biofilms, especially from contaminated catheters. These species a high level antibiotic resistance; thus, these infections challenging treat resulting in excess...
Abstract Coccidioides species are fungal pathogens that can cause a widely varied clinical manifestation from mild pulmonary symptom to disseminated, life-threatening disease. We have previously created subunit vaccine by encapsulating recombinant coccidioidal Ag (rCpa1) in glucan–chitin particles (GCPs) as an adjuvant-delivery system. The GCP-rCpa1 has shown elicit mixed Th1 and Th17 response confers protection against coccidioidomycosis mice. In this study, we further delineated the...
Dialyzable leukocyte extract (DLE) is one of the immunological agents used as an adjuvant in cancer therapy; it has been associated with improved quality life during chemotherapy. Based on these previous findings and observed clinical benefits attributed to DLE other types cancer, we investigated its effects a therapy breast patients who received only chemotherapy, compared administered bovine (bDLE) adjuvant. This study included 43 were about begin group was divided follows: 25 chemotherapy...
Bacitracin and the membrane-impermeant thiol reagent 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) are agents known to inhibit protein disulfide isomerase (PDI), a cell-surface critical in HIV-1 entry therefore they fusion inhibitors (FI). Here we investigated possibility that or DTNB might have other antiviral activities besides FI. By means of residual activity assays, found both compounds showed only viruses T-tropic strain. Cell-based assays inhibition on HeLa-CD4-LTR-β-gal (CD4) HL2/3 cells...
Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis (AHPNS) is a disease produced by gram-negative bacteria Vibrio parahaemolyticus ( V. ), which has caused declines in worldwide production of white shrimp Litopenaeus vannamei L. ). In this work, we propose the implementation silver nanoparticles (AgNPs) synthesized with Rumex hymenosepalus (Rh) extract as an alternative on control. AgNPs were characterized UV-Vis spectroscopy, scanning electron microscopy (SEM), transmission...