A5015669067- Neurobiology and Insect Physiology Research
- Circadian rhythm and melatonin
- Sleep and Wakefulness Research
- Insect and Arachnid Ecology and Behavior
- Plant Molecular Biology Research
- Plant and Biological Electrophysiology Studies
- Plant and animal studies
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience of respiration and sleep
- Photoreceptor and optogenetics research
- Bat Biology and Ecology Studies
- Zebrafish Biomedical Research Applications
- Physiological and biochemical adaptations
- Sleep and related disorders
- Neuroscience and Neuropharmacology Research
University of California, Los Angeles
2017-2024
University of Oxford
2014-2018
Washington University in St. Louis
2009-2014
Sleep is believed to play an important role in memory consolidation. We induced sleep on demand by expressing the temperature-gated nonspecific cation channel Transient receptor potential (UAS-TrpA1) neurons, including those with projections dorsal fan-shaped body (FB). When temperature was raised 31°C, flies entered a quiescent state that meets criteria for identifying sleep. 4 hours after massed-training protocol courtship conditioning not capable of inducing long-term (LTM) itself,...
Sleep-promoting neurons in the dorsal fan-shaped body (dFB) of Drosophila are integral to sleep homeostasis, but how these cells impose on organism is unknown. We report that dFB communicate via inhibitory transmitters, including allatostatin-A (AstA), with interneurons connecting superior arch ellipsoid central complex. These "helicon cells" express galanin receptor homolog AstA-R1, respond visual input, gate locomotion, and inhibited by AstA, suggesting promote rest suppressing visually...
Sleep is important for memory consolidation and responsive to waking experience. Clock circuitry uniquely positioned coordinate interactions between processes underlying sleep need. Flies increase both after exposure an enriched social environment protocols that induce long-term memory. We found flies mutant rutabaga, period, blistered were deficient experience-dependent increases in sleep. Rescue of each these genes within the ventral lateral neurons (LNVs) restores increased enrichment....
Sleep is an evolutionarily conserved state that supports brain functions, including synaptic plasticity, in species across the animal kingdom. Here, we examine neuroanatomical and cell-type distribution of presynaptic scaling fly after sleep loss. We previously found loss drives accumulation active zone scaffolding protein Bruchpilot (BRP) within cholinergic Kenyon cells Drosophila melanogaster mushroom body (MB), but not other classes MB neurons. To test whether similar cell type–specific...
Recent human studies suggest that genetic polymorphisms allow an individual to maintain optimal cognitive functioning during sleep deprivation. If such were not associated with additional costs, selective pressures would these alleles spread through the population evolutionary alternative emerge. To determine whether there are indeed costs resiliency loss, we challenged natural allelic variants of foraging gene ( for ) either deprivation or starvation. Flies high levels Protein Kinase G...
The central complex (CX) is a midline-situated collection of neuropil compartments in the arthropod brain, implicated higher-order processes such as goal-directed navigation. Here, we provide systematic genetic-neuroanatomical analysis ellipsoid body (EB), compartment which represents major afferent portal Drosophila CX. volume EB, along with its prominent input compartment, called bulb, subdivided into precisely tessellated domains, distinguishable based on intensity global marker...
(Neuron 81, 860–872; February 19, 2014) We thank Douglas Armstrong, Hugo Bellen, Ronald Davis, Ulrike Heberlein, Martin Heisenberg, Liqun Luo, Gerald Rubin, and Helen Skaer for fly strains. In a regrettable oversight, some strains were credited to stock centers rather than the original sources. The correct attributions are as follows: cv-cMB03717, cv-cMB01956, cv-cDG20401 (Bellen et al., 2011Bellen H.J. Levis R.W. He Y. Carlson J.W. Evans-Holm M. Bae E. Kim J. Metaxakis A. Savakis C. Schulze...
The Serotonin Transporter (SERT) regulates extracellular serotonin levels and is the target of most current drugs used to treat depression. mechanisms by which inhibition SERT activity influences behavior are poorly understood. To address this question in model organism Drosophila melanogaster , we developed new loss function mutations ( dSERT) . Previous studies both flies mammals have implicated as an important neuromodulator sleep, our newly generated dSERT mutants show increase total...
Aging has been linked with decreased neural plasticity and memory formation in humans laboratory model species such as the fruit fly, Drosophila melanogaster. Here, we examine plastic responses following social experience a high-throughput method to identify interventions that prevent these impairments.Wild-type transgenic melanogaster.Young (5-day old) or aged (20-day adult female were housed socially enriched (n = 35-40) isolated environments, then assayed for changes sleep structural...
Abstract The relationship between sleep and memory is an active topic of investigation. In this context, we demonstrate that enhancing restores to flies with ablated Mushroom Bodies (MB), a key center; consistent across several assays. Mapping the underlying circuitry reveals circadian modulation subset Dopaminergic neurons (DANs) modulate aversive learning. Using imaging, show MB-ablation disrupts, time day these are most responsive. Knocking down receptor for clock output signal,...
Sleep is essential for a variety of plastic processes, including learning and memory. However, the consequences insufficient sleep on circuit connectivity remain poorly understood. To better appreciate effects loss synaptic across memory-encoding circuit, we examined changes in distribution markers Drosophila mushroom body (MB). Protein-trap tags active zone components indicate that recent time inversely correlated with Bruchpilot (BRP) abundance MB lobes; elevates BRP while induction...
Recent work in
Sleep is broadly conserved across the animal kingdom but can vary widely between species. It currently unclear which selective pressures and regulatory mechanisms influence differences in sleep The fruit fly Drosophila melanogaster has become a successful model system for examining regulation function, little known about patterns many related Here, we find that species with adaptations to extreme desert environments, including D. mojavensis, exhibit strong increases baseline compared...
Summary Recent work in Drosophila has uncovered several neighboring classes of sleep-regulatory neurons within the central complex. However, logic connectivity and network motifs remains limited by incomplete examination relevant cell types. Using a recent genetic-anatomical classification ellipsoid body ring neurons, we conducted thermogenetic screen to assess sleep/wake behavior discovered two opposing populations: sleep-promoting R3m wake-promoting R3d neurons. Activation these influences...
Abstract Sleep is broadly conserved across the animal kingdom, but can vary widely between species. It currently unclear which types of selective pressures and sleep regulatory mechanisms influence differences in The fruit fly Drosophila melanogaster has become a successful model system for examining regulation function, little known about patterns need many related Here, we find that mojavensis , species adapted to extreme desert environments, exhibits strong increases compared D....