A5024836826- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Genetics and Neurodevelopmental Disorders
- Diet and metabolism studies
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Histone Deacetylase Inhibitors Research
- Digestive system and related health
- Lipoproteins and Cardiovascular Health
- Circular RNAs in diseases
- Genomic variations and chromosomal abnormalities
- Spine and Intervertebral Disc Pathology
- Genomics and Rare Diseases
- Helicobacter pylori-related gastroenterology studies
- Musculoskeletal pain and rehabilitation
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Pain Mechanisms and Treatments
- Folate and B Vitamins Research
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- Diabetes, Cardiovascular Risks, and Lipoproteins
Universidade Federal de São Paulo
2015-2024
McGill University
2012-2020
Universidade Federal da Paraíba
2016
Creative Research Enterprises (United States)
2016
Universidade Federal do Pará
2016
Centro de Genética Clínica
2016
Douglas Mental Health University Institute
2012-2015
Microsoft (Brazil)
2015
University of British Columbia
2015
Dalhousie University
2015
Longevity related genes were investigated concerning promoter methylation. SIRT3, SMARCA5, HTERT and CDH1 promoters analyzed in peripheral blood relation to gender, age Alzheimer's disease (AD). Methylation Specifc PCR assay (MSP) was use
To evaluate the suitability of reference genes in gastric tissue samples and cell lines.The ACTB, B2M, GAPDH, RPL29, 18S rRNA was assessed 21 matched pairs neoplastic adjacent non-neoplastic tissues from patients with adenocarcinoma, 27 normal without cancer, 4 lines using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The ranking best single combination determined by NormFinder, geNorm™, BestKeeper, DataAssist™. In addition, GenEx software used to...
Here, we first evaluated SMARCA5 expression and promoter DNA methylation in gastric carcinogenesis. Immunohistochemistry methylation-specific PCR were analyzed 19 48 normal mucosa 52 92 cancer samples, respectively. We observed higher immunoreactivity of samples than mucosa. Moreover, was associated with the absence protein expression. Our findings suggest that has a potential role proliferation malignancy
Gastric cancer is still the second leading cause of cancer-related death worldwide, even though its incidence and mortality have declined over recent few decades. Epigenetic control using histone deacetylase inhibitors, such as trichostatin A (TSA), a promising therapy. This study aimed to assess messenger RNA (mRNA) levels three deacetylases (HDAC1, HDAC2, HDAC3), two acetyltransferases (GCN5 PCAF), possible targets these modifiers (MYC CDKN1A) in 50 matched pairs gastric tumors...
Abstract Genes implicated in neurodevelopmental disorders (NDDs) important cognition and behavior may have convergent function several cellular pathways been implicated, including protein translational control, chromatin modification, synapse assembly maintenance. Here, we test the effects of methyl-CpG binding domain 5 ( MBD5) special AT-rich 2 SATB2) reduced dosage human neural stem cells (NSCs), two genes 2q23.1 2q33.1 deletion syndromes, respectively, to develop a generalized model for...
Gene expression dysregulation in the brain has been associated with bipolar disorder through candidate gene and microarray studies, but questions remain about isoform-specific role of noncoding RNAs whose importance suggested recently not yet characterized for disorder.The authors used RNA sequencing, a powerful technique that captures complexity expression, postmortem tissue from anterior cingulate cortex 13 case subjects matched comparison subjects. Differential was computed, global...
Gastric cancer is the second most prevalent cause of death worldwide. DNA methylation a common event in gastric carcinogenesis. hTERT seems to be rate-limiting determinant telomerase activation, which responsible for stability and life span. hypermethylation has been associated with expression. In present study, we investigated promoter status protein expression normal mucosa samples. One hundred nine 53 samples were through methylation-specific PCR. Immunohistochemistry was analysed using...
Aims: To evaluate H3K9 acetylation and gene expression profiles in three brain regions of Alzheimer's disease (AD) patients elderly controls, to identify AD region-specific abnormalities. Methods: Brain samples auditory cortex, hippocampus cerebellum from controls underwent chromatin immunoprecipitation sequencing, RNA sequencing network analyses. Results: We found a hyperacetylation slight hypoacetylation hippocampus. The transcriptome revealed differentially expressed genes the cortex....
Aberrant methylation has been reported in several neoplasias, including gastric cancer. The methyl-CpG-binding domain (MBD) family proteins have implicated the chromatin remodeling process, leading to modulation of gene expression. To evaluate role MBD2 and MBD3 carcinogenesis possible association with clinicopathological characteristics, we assessed mRNA levels promoter patterns tissues. In this study, were determined by RT-qPCR 28 neoplastic adjacent nonneoplastic 27 gastritis...
Aim: To analyze gene expression and copy number of five miRNAs (miR-1204, miR-1205, miR-1206, miR-1207 miR-1208) localized in this chromosome region gastric cancer (GC). Materials & methods: 65 paired neoplastic non-neoplastic specimens collected from GC patients 20 tissues cancer-free individuals were included study. The levels the accessed by real time qPCR correlated. Results: MiR-1207-3p, miR-1207-5p miR-1208 upregulated approximately 50% tumors relation to those adjacent tissues....