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Abstract Background Antibiotic resistance in Helicobacter pylori contributes to failure eradicating the infection and is most often due point missense mutations a few key genes. Methods The antibiotic susceptibility profiles of H. isolates from 46 Pakistani patients were determined by Etest. Resistance pathogenicity genes amplified, sequences analyzed determine presence mutations. Results A high percentage (73.9%) resistant metronidazole (MTZ), with considerable clarithromycin (CLR; 47.8%)...

Hospital environmental surfaces are potential reservoirs for transmitting hospital-associated pathogens. This study aimed to profile microbiomes and antibiotic resistance genes (ARGs) from hospital using 16S rRNA amplicon metagenomic sequencing at a tertiary teaching in Malaysia. Samples were collected patient sinks healthcare staff counters surgery orthopaedic wards. The samples’ DNA subjected shotgun identify bacterial taxonomic profiles, genes, virulence factor pathways. richness was more...

Antibiotic resistance is increasing worldwide, and it has been regarded as the main factor reducing efficacy of Helicobacter pylori therapy. The aim this study was to determine phenotype genotype antibiotic-resistant strains H. in Malaysian population evaluate impact antibiotic eradication outcome. One hundred sixty-one isolates were analysed study. Metronidazole, clarithromycin, fluoroquinolone, amoxicillin tetracycline susceptibilities determined by Etest. PCR followed DNA sequencing...

Aims and objectives: Helicobacter pylori has been classified as high priority pathogen by the WHO in 2017. The emergence of antibiotic-resistant strains is one main causes treatment failure H. infection. This study determined characterized primary secondary resistances Malaysia. Materials methods: Gastric biopsies from antrum (n=288) corpus (n=283) were obtained 288 patients who underwent endoscopy at Universiti Kebangsaan Malaysia Medical Center (UKMMC), Kuala Lumpur, Antibiotic...

Antimicrobial resistance in Acinetobacter baumannii is a growing public health concern and an important pathogen nosocomial infections. We investigated the genes involved to carbapenems cephalosporins clinical A. isolates from tertiary medical centre Malaysia. was isolated 167 specimens identified by sequencing of 16S rRNA rpoB genes. The MIC for imipenem, meropenem, ceftazidime cefepime were determined E-test method. presence carbapenemase cephalosporinase PCR. predominantly nonsusceptible...

Owing to its strong acid production, the stomach was known be a bacteria-free organ for many years.On other hand, presence of Helicobacter pylori (H.pylori) and acid-resistant microbiota that are persist in challenged this.It is now recognized existence H. non-H.pylori species have been linked improvement gastric disease; despite this, there little published data on interaction bacterial flora resultant effect health.The has unique including five major phyla, such as Firmicutes,...

Multidrug-resistant (MDR) Acinetobacter baumannii has increasingly emerged as an important nosocomial pathogen. The aim of this study was to determine the resistance profiles and genetic diversity in A. clinical isolates a tertiary medical center Malaysia. minimum inhibitory concentrations carbapenems (imipenem meropenem), cephalosporins (ceftazidime cefepime), ciprofloxacin were determined by E-test. PCR sequencing carried out for detection antibiotic genes mutations. Clonal relatedness...

The emergence of multidrug-resistant Helicobacter pylori has undermined eradication strategies to prevent the development gastric cancer. This study was conducted estimate prevalence secondary antibiotic resistance H. in urban multicultural areas Malaysia.From January 2017 December 2021, biopsies from 218 patients with a history failure were sent our laboratory for susceptibility testing. A minimal inhibitory concentration determined six antibiotics, namely metronidazole, clarithromycin,...

We have defined DNA repeat variability in the 3′-terminus of cagA gene Helicobacter pylori strains from Malaysian patients different ethnicities. identified alleles based on EPIYA repeats. types A-B-D and A-B-B-D are more similar to sequence Japanese strains, whereas A-B-C, A-B-C-C, A-B A-C displayed similarity strain 26695 sequences. A significant association was found between genotypes patients’ ethnicity, with type being predominantly isolated Chinese A-B-C Malays Indians. Our data...

More than half of the world's population is infected with Helicobacter pylori, which can cause chronic gastritis. WHO has regarded clarithromycin-resistant H. pylori as a high priority pathogen. Hence, accurate diagnosis and detection clarithromycin- levofloxacin-resistant strains essential for proper management infection. The objective this study was to develop optimize multiplex quantitative PCR assay mutations associated clarithromycin levofloxacin resistance in directly from gastric...

Helicobacter pylori infection of a distinct subtype cagA may lead to different pathological manifestation. The aim this study is determine the presence gene and its variants in H. among ethnic groups effect on gastroduodenal diseases. Overall detection 205 clinical isolates was 94%. Variations size 3' region were examined 192 Malaysian cagA-positive strains. Results showed that three differing fragment length PCR products detected designated as type A (621-651bp), B (732-735bp) C (525 bp)....

Abstract Background and aim: Polymorphisms of Helicobacter pylori cagA vacA genes do exist may contribute to differences in H. infection gastroduodenal diseases among races the Malaysian population. This study was conducted characterize polymorphisms Methods: A total 110 isolates were genotyped by PCR sequenced for PCR‐RFLP . Results: East Asian predominantly detected (64.5%), whereas s1m1 s1m2 alleles 60.9 37.3% strains, respectively. statistical association between type with patients’...

Respiratory infections represent a major public health problem worldwide. The study aimed to determine the prevalence of respiratory syncytial and influenza virus analyzed in respect demography clinical perspective. Methods : specimens were processed by cell culture immunofluorescent assay (IFA) real-time reverse transcriptase-PCR (rRT-PCR) for detection viruses. Results Out 505 189 (37.8%) positive, which RSV was positive 124(24.8%) cases A 65(13%) cases. Positive based on demography: age,...

Expression of cluster differentiation (CD) antigens changes according to disease status and inflammation. Profiles CD expression in gastric cancer patients are different based on the H. pylori infection.We conducted this study profile antigen markers adenocarcinoma cells (AGS cell line) infected with distinct cytotoxin-associated gene A (cagA) genotypes clinical isolates.The AGS were isolates cagA genotypes, was determined using DotScan™ antibody microarray. Formation "hummingbird" phenotype...