A5001931265- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- Influenza Virus Research Studies
- Cancer Research and Treatments
- Respiratory viral infections research
- Protein purification and stability
- Immune Cell Function and Interaction
- DNA and Nucleic Acid Chemistry
- Immune responses and vaccinations
- interferon and immune responses
- Inflammasome and immune disorders
- Receptor Mechanisms and Signaling
- Cardiac Fibrosis and Remodeling
- Neuropeptides and Animal Physiology
- Cancer Genomics and Diagnostics
Institute of Cell Biology
2020
Leipzig University
2019
Meridian Bioscience (United States)
2010
University of Nottingham
2006
Medical University of Vienna
2006
Comprehensive Cancer Center Vienna
2004
Boehringer Ingelheim (Austria)
1997-2003
University of Vienna
2001
Boehringer Ingelheim (Germany)
1997-2000
Teikyo University
1995-1996
Background Efficient gene transfer is a major challenge for non-viral therapy. Understanding how vectors initiate expression could lead to the development of new future with enhanced efficacy. Methods Linear or branched polyethylenimine (PEI)/DNA complexes were generated in varying salt conditions and their transfection efficiencies compared vitro vivo using reporter genes, luciferase green fluorescent protein, rhodamine labeled DNA (pGeneGrip™). Results The efficiency linear PEI22/DNA was...
Patients infected with SARS-CoV-2 show a wide spectrum of clinical manifestations ranging from mild febrile illness and cough up to acute respiratory distress syndrome, multiple organ failure, death. Data patients severe compared symptoms indicate that highly dysregulated exuberant inflammatory responses correlate severity disease lethality. Epithelial-immune cell interactions elevated cytokine chemokine levels, i.e. storm, seem play central role in lethality COVID-19. The present...
Background Efficient and target-specific in vivo gene delivery is a major challenge therapy. Compared to cell culture application, faces variety of additional obstacles such as anatomical size constraints, interactions with biological fluids extracellular matrix, binding broad non-target types. Methods Polycation-based vectors, including adenovirus-enhanced transferrinfection (AVET) transferrin-polyethylenimine (Tf-PEI), were tested for into subcutaneously growing tumors after local systemic...
Tumor-targeting DNA complexes which can readily be generated by the mixing of stable components and freeze-thawed would very advantageous for their subsequent application as medical products. Complexes were plasmid DNA, linear polyethylenimine (PEI22, 22 kDa) main condensing agent, PEG-PEI (poly(ethylene glycol)-conjugated PEI) surface shielding, Tf-PEG-PEI (transferrin-PEG-PEI) to provide a ligand receptor-mediated cell uptake. Within shielding conjugates, PEG chains varying size (5, 20, or...
With the aim of generating gene delivery systems for tumor targeting, we have synthesized a conjugate consisting polyethylenimine (PEI) covalently modified with epidermal growth factor (EGF) peptides. Transfection efficiency was evaluated and compared to native PEI in three cell lines: KB epidermoid carcinoma cells, CMT-93 rectum Renca-EGFR renal cells. Depending on line, incorporation EGF resulted an up 300-fold increased transfection efficiency. This ligand-mediated enhancement competition...
The SARS-CoV-2 Omicron variants have replaced all earlier variants, due to increased infectivity and effective evasion from infection- vaccination-induced neutralizing antibodies. Compared of concern (VoCs), the show high TMPRSS2-independent replication in upper airway organs, but lower lungs mortality rates. shift cellular tropism towards pathogenicity was hypothesized correlate with a toll-like receptor (TLR) activation, although underlying molecular mechanisms remained undefined. In...
Systemic tumor-targeted gene delivery is attracting increasing attention as a promising alternative to conventional therapeutical strategies. To be considered viable option, however, the respective transgene has administered with high tumor specificity. Here, we describe novel polyethylenimine (PEI)-based DNA complexes, shielded by covalent attachment of polyethylene glycol (PEG), that make use epidermal growth factor (EGF) ligand for targeting EGF receptor-expressing human hepatocellular...