A5083380034- Advanced Proteomics Techniques and Applications
- Homicide, Infanticide, and Child Abuse
- Suicide and Self-Harm Studies
- Mass Spectrometry Techniques and Applications
- Grief, Bereavement, and Mental Health
- Glycosylation and Glycoproteins Research
- Analytical Chemistry and Chromatography
- Enzyme Structure and Function
- Cancer, Hypoxia, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Child Abuse and Trauma
- Mitochondrial Function and Pathology
- Food Allergy and Anaphylaxis Research
- Protein purification and stability
- Immune cells in cancer
- PI3K/AKT/mTOR signaling in cancer
- Metabolomics and Mass Spectrometry Studies
- Pharmacogenetics and Drug Metabolism
- Retinal Development and Disorders
- Child Abuse and Related Trauma
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Mast cells and histamine
- Melanoma and MAPK Pathways
- Monoclonal and Polyclonal Antibodies Research
- Dye analysis and toxicity
ETH Zurich
2016-2021
University of Vienna
2005-2019
University of Southern Denmark
2010-2014
Medical University of Vienna
2009-2013
Norwegian Institute of Bioeconomy Research
2011
Philipps University of Marburg
1995
Living systems integrate biochemical reactions that determine the functional state of each cell. Reactions are primarily mediated by proteins. In proteomic studies, these have been treated as independent entities, disregarding their higher-level organization into complexes affects activity and/or function and is thus great interest for biological research. Here, we describe implementation an integrated technique to quantify cell-state-specific changes in physical arrangement protein...
Familicides have received relatively little attention and are mostly discussed in studies with broader aims. Here, we reviewed 67 from 18 countries on familicides, which an offender killed or attempted to kill their current former spouse/intimate partner one more of biological stepchildren. We conducted a systematic literature search PubMed, PsycINFO, Google Scholar. Eight investigated familicide specifically, while the remaining reported cases as subsample. retrieved data offenders' gender,...
Physiological processes in multicellular organisms depend on the function and interactions of specialized cell types operating context. Some these are rare thus obtainable only minute quantities. For example, tissue-specific stem progenitor cells numerically scarce, but functionally highly relevant, fulfill critical roles development, tissue maintenance, disease. Whereas low numbers routinely analyzed by genomics transcriptomics, corresponding proteomic analyses have so far not been possible...
To assess rates of neonaticide after the implementation a preventative 'anonymous delivery' law in mid-2001 Austria. Women are allowed to access antenatal care and give birth hospital anonymously, without showing any ID free charge.Retrospective study.A complete census police-reported neonaticides was obtained from police statistics Austria, Sweden Finland.All reported police, 1991-2009.Neonaticide before (1991-2001) (2002-2009) introduction anonymous delivery legislation per 100 000...
Mass spectrometry has become a valuable method for studying structural dynamics of proteins in solution by measuring their backbone amide hydrogen/deuterium exchange (HDX) kinetics. In typical experiment one or more are incubated deuterated buffer at physiological conditions. After given period deuteration, the reaction is quenched acidification (pH 2.5) and cooling (0 °C) protein (or digest thereof) analyzed mass spectrometry. The unavoidable loss deuterium (back-exchange) that occurs under...
Filicide is the tragic crime of murdering one's own child. Previous research has found that offending parents are commonly depressed and suicide often associated as an actual act or intention. Yet, filicide underreported previous studies have been strained with methodological problems. No comprehensive international on presented in literature until now. This was a descriptive, comprehensive, register-based study all filicides Austria Finland during 1995-2005. Filicide-suicide cases were also...
Filicide is the act of killing one's own child. We propose a new classification subtypes filicide and make suggestions for helping its prevention. This binational, register-based study covers all cases their perpetrators in Austria Finland years 1995–2005. The following variables emerged as differentiating factors: gender, age victim offender, circumstances before during offence, offender's socioeconomic criminal background, childhood conduct disorder. Latent class analysis (LCA) was used to...
Abstract Defined conditions of EOF along with different pH values the BGE were compared for purpose analyzing glycopetides by CZE coupled to MS (CZE‐MS). Hyphenation involved ESI as well MALDI, and single‐stage multistage applied. Variation was accomplished selecting appropriate coatings capillary, namely hexadimethrine bromide (HDMB) HDMB/dextran sulfate. A high reproducible anodic cathodic EOF, respectively, obtained in both approaches, allowing detection analytes net positive negative...
This study aims to identify differences between single and repeat perpetrators of filicide by using register-based data. The used register-based, comprehensive, nationwide data from both Austria Finland. current covers 23 perpetrators, 20 3 with a total 28 victims. All victims had maximum age 24 h all were women. Every third victim neonaticide was case. older; higher number children over their lifespan, some whom lived them; more likely live within established family structures; levels...
Abstract This study aimed to discover gender differences in filicide offenders' psychiatric diagnoses, history, and other mental health-related variables. nationwide register-based covered all offenders (n = 124) Austria Finland 1995–2005. There were 79 female 45 male offenders. Both genders had an equally challenged childhood. The filicidal fathers older than the mothers, mean ages 37.5 31.6 years, respectively (p < 0.001). Filicidal committed suicide at crime scene more often mothers (38%...
Abstract Affinity‐CIEF has been applied to characterize a recombinant product of the major birch pollen allergen Betula verrucosa isoform 1a (Bet v 1a) immuno‐chemically. For this purpose mAbs IgG‐type have produced in‐lab from two murine hybridoma lines, specified as clones 2 and 5.1. Both IgG were characterized by SDS‐PAGE, MALDI‐TOF‐MS CIEF. The purified solutions had be dialysed against 10 mmol/L phosphate (pH 7.4) prevent precipitation ensure appropriate CIEF separation. tested...
Abstract We have applied an arginine‐specific labeling technique to the study of peptides by matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS). The reaction converts guanidine group arginine side chain reacting it with 2,3‐butanedione and arylboronic acid. Despite general chemical lability tag under acidic conditions, was possible employ matrices like α ‐cyano‐4‐hydroxycinnamic acid without adverse effects, using thin‐layer for preparation. After optimizing method...
Recently, data-independent acquisition (DIA) MS has gained popularity as a qualitative-quantitative workflow for proteomics. One outstanding problem in the analysis of DIA-MS data is alignment chromatographic retention times across multiple samples, which facilitates peptide identification and accurate quantification. Here, we present novel hybrid (profile-based feature-based) algorithm LC-MS test it on sequential windowed all theoretical fragment ion mass spectra (SWATH) (a type DIA) data....
This study analyses the psychological, clinical and criminal characteristics of neonaticide focusing on court verdicts with aim formulating recommendations for judicial guidelines. was register based, comprising all known neonaticides in Austria Finland between 1995 2005. The cases ( n = 28) were obtained by screening death certificates from coroner departments analysing them alongside further reports available. Five out 21 convicted offenders imprisoned an average sentence 1.65 years. A...