A5024117382- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Lipoproteins and Cardiovascular Health
- Design Education and Practice
- Atherosclerosis and Cardiovascular Diseases
- Lysosomal Storage Disorders Research
- Statistical Methods and Inference
- Caveolin-1 and cellular processes
- Financial Risk and Volatility Modeling
- Sustainable Supply Chain Management
- Statistical Methods and Bayesian Inference
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- S100 Proteins and Annexins
- Chemistry and Chemical Engineering
- Interdisciplinary Research and Collaboration
- Advancements in Transdermal Drug Delivery
- Drug Solubulity and Delivery Systems
- Microencapsulation and Drying Processes
- Cell Image Analysis Techniques
- Diabetes, Cardiovascular Risks, and Lipoproteins
University of Southern Denmark
2014-2024
Aalborg University
2023
Cornell University
2012-2019
Visby lasarett
2016
Nonvesicular transport of cholesterol plays an essential role in the distribution and regulation within cells, but it has been difficult to identify key intracellular transporters. The steroidogenic acute regulatory-related lipid-transfer (START) family proteins is involved several pathways nonvesicular trafficking sterols. Among them, STARD4 shown increase cholesteryl ester formation controlled at transcriptional level by sterol levels cells. We found that very efficient transporting...
Plant protein ingredients from similar sources can vary in functionality not only because of compositional differences, but also differences their structure depending on processing history. It is essential to understand these distinctions develop novel food emulsion using plant proteins. hypothesized that differing interfacial properties be attributed structures, aggregation, and colloidal states. The adsorption behavior a commercial isolate, homogenized or non-homogenized, was compared...
Abstract Background Fluorescence loss in photobleaching (FLIP) is a widely used imaging technique, which provides information about protein dynamics various cellular regions. In FLIP, small region repeatedly illuminated by an intense laser pulse, while images are taken with reduced power time lag between the bleaches. Despite its popularity, tools lacking for quantitative analysis of FLIP experiments. Typically, user defines regions interest (ROIs) further subjective and does not allow...
Cholesterol is an essential constituent of membranes in mammalian cells. The plasma membrane and the endocytic recycling compartment (ERC) are both highly enriched cholesterol. abundance distribution cholesterol among organelles tightly controlled by a combination mechanisms involving vesicular nonvesicular sterol transport processes. Using fluorescent analogue dehydroergosterol, we examined between ERC using fluorescence recovery after photobleaching novel efflux assay. We found that these...
STARD4, a member of the evolutionarily conserved START gene family, has been implicated in nonvesicular intracellular transport cholesterol. However, direction and membranes with which this protein interacts are not clear. We present studies STARD4 function using small hairpin RNA knockdown technology to reduce expression HepG2 cells. In cholesterol-poor environment, we found that reduction leads retention cholesterol at plasma membrane, endoplasmic reticulum-associated cholesterol,...
Monocyte-derived cells use an extracellular, acidic, lytic compartment (a lysosomal synapse) for initial degradation of large objects or species bound to the extracellular matrix. Akin osteoclast bone, catabolism is used by macrophages degrade aggregates low density lipoprotein (LDL) similar those encountered during atherogenesis. However, unlike catabolism, synapse a highly dynamic and intricate structure. In this study, we high resolution three dimensional imaging visualize compartments...
Aqueous suspensions of poorly soluble, crystalline drug particles in the sub-micron range hold ability to regulate release for a defined period time after e.g., intramuscular, or subcutaneous administration, working as an eminent formulation strategy preparation long-acting injectables. are typically prepared by top-down approaches, wet bead media milling high-pressure homogenization, containing active pharmaceutical compound and surfactants and/or polymers stabilization purposes. Currently,...
The proangiogenic members of VEGF family and related receptors play a central role in the modulation pathological angiogenesis. Recent insights indicate that, due to strict biochemical functional relationship between VEGFs receptors, development new generation agents able target contemporarily more than one member might amplify antiangiogenic response representing an advantage term therapeutic outcome. To identify molecules that are prevent interaction with we have screened small molecule...
The endocytic pathway is a complex network of highly dynamic organelles, which has been traditionally studied by quantitative fluorescence microscopy. data generated this method can be overwhelming and its analysis, even for the skilled microscopist, tedious error-prone. We developed SpatTrack, an open source, platform-independent program collecting variety methods analysis vesicle dynamics distribution in living cells. SpatTrack performs 2D particle tracking, trajectory fitting diffusion...
Repair of damaged plasma membrane in eukaryotic cells is largely dependent on the binding annexin repair proteins to phospholipids. Changing biophysical properties may provide means compromise annexin-mediated and sensitize injury. Since, cancer experience heightened stress are more efficient repair, inhibiting approaches damage cell death. Here, we show that derivatives phenothiazines, which have widespread use fields psychiatry allergy treatment, strongly mechanical-, chemical-,...
We implement Bayesian model selection and parameter estimation for the case of fractional Brownian motion with measurement noise a constant drift. The approach is tested on artificial trajectories shown to make estimates that match well underlying true parameters, while has preference simple models when are finite. applied observed vesicles diffusing in Chinese hamster ovary cells. Here it supplemented goodness-of-fit test, which able reveal statistical discrepancies between predictions.
Rapid membrane repair is required to ensure cell survival after rupture of the plasma membrane. The annexin family proteins involved in (PMR) and activated by influx Ca
Abstract Background Cholesterol is an important membrane component, but our knowledge about its transport in cells sparse. Previous imaging studies using dehydroergosterol (DHE), intrinsically fluorescent sterol from yeast, have established that vesicular and non-vesicular modes contribute to trafficking the plasma membrane. Significant photobleaching, however, limits possibilities for in-depth analysis of dynamics DHE. Co-trafficking with DHE recently introduced cholesterol analog...
Summary Single particle tracking (SPT) is becoming a standard method to extract transport parameters from time‐lapse image sequences of fluorescent vesicles in living cells. Another obtain these data temporal correlation spectroscopy (TICS), but this less often used for measurement intracellular vesicle transport. Here, we present an extensive comparison SPT and TICS. First examine the effect photobleaching, shading noise on TICS analysis using simulated sequences. To end, developed simple...
Low-density lipoprotein (LDL) deposition, aggregation and retention in the endothelial sub-intima are critical initiating events during atherosclerosis. Macrophages digest aggregated LDL (agLDL) through a process called exophagy. High-density (HDL) plays an atheroprotective role, but studies attempting to exploit it therapeutically have been unsuccessful, highlighting gaps our current understanding of HDL function. Here, we characterized role exophagy agLDL. We find that atherosclerotic...
The active hallucinogen of magic mushrooms, psilocin, is being repurposed to treat nicotine addiction and treatment-resistant depression. Psilocin belongs the tryptamine class psychedelic compounds which include hormone serotonin. It believed that psilocin exerts its effect by binding serotonin 5-HT2A receptor. However, recent in-vivo evidence suggests may employ a different mechanism exert effects. Membrane-mediated receptor desensitization neurotransmitter receptors one such mechanism. We...