A5017975271- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advancements in Transdermal Drug Delivery
- Luminescence and Fluorescent Materials
- Bee Products Chemical Analysis
- Animal Vocal Communication and Behavior
- Spectroscopy and Quantum Chemical Studies
- Spectroscopy and Chemometric Analyses
- Erythrocyte Function and Pathophysiology
- Sphingolipid Metabolism and Signaling
- Organic Electronics and Photovoltaics
- Nonlinear Optical Materials Studies
- Porphyrin and Phthalocyanine Chemistry
- Photochromic and Fluorescence Chemistry
- Mechanical and Optical Resonators
- Animal Behavior and Reproduction
- Elasticity and Material Modeling
- Silk-based biomaterials and applications
- Quantum optics and atomic interactions
- Marine Biology and Environmental Chemistry
- Plasmonic and Surface Plasmon Research
- Cellular Mechanics and Interactions
- Cardiovascular Disease and Adiposity
- Force Microscopy Techniques and Applications
University of Southern Denmark
2016-2025
The University of Texas at Dallas
2024
The University of Texas Southwestern Medical Center
2022
José Rizal University
2022
University of Washington
2022
Asian Development Bank
2022
Norwegian Institute of Bioeconomy Research
2013-2019
Odense Municipality
2009-2017
Doheny Eye Institute
2012
University College South Denmark
2007
The skin barrier is fundamental to terrestrial life and its evolution; it upholds homeostasis protects against the environment. Skin capacity controlled by lipids that fill extracellular space of skin's surface layer--the stratum corneum. Here we report on determination molecular organization lipid matrix in situ, near-native state, using a methodological approach combining very high magnification cryo-electron microscopy (EM) vitreous section defocus series, modeling, EM simulation. are...
The use of nanoparticles in foods, materials, and clinical treatments has increased dramatically the past decade. Because possibility human exposure to nanoparticles, there is an urgent need investigate molecular mechanisms underlying cellular responses that might be triggered. Such information necessary assess potential health risks arising from for developing new formulations next generation treatments. Using mass spectrometry-based proteomic technologies complementary techniques (e.g.,...
The liver is essential for normal fatty acid utilization during fasting. Circulating acids are taken up by hepatocytes and esterified as triacylglycerols either oxidative metabolization ketogenesis or export. Whereas the regulation of oxidation in well understood, uptake retention non-esterified not. Here, we show that murine hepatic stellate cells (HSCs) their abundantly expressed plasmalemma vesicle-associated protein (PLVAP) control substrate preference fasting energy metabolism....
Abstract A new route to bottom‐up organic nanotechnology is presented. Molecular building blocks with specific optoelectronic properties are designed and grown via directed self‐assembly arrays of morphologically controlled light‐emitting nanofibers on template surfaces. The fibers can be easily transferred from the growth substrate device platforms either as single entities or ordered arrays. Due extraordinary flexibility in design their they serve key elements next‐generation nanophotonic devices.
Uropathogenic Escherichia coli (UPEC) strains are capable of invading bladder epithelial cells (BECs) on the luminal surface. Based primarily studies in mouse models, invasion is proposed to trigger an intracellular uropathogenic cascade involving bacterial proliferation followed by escape elongated, filamentous bacteria from colonized BECs. UPEC filaments epithelium able revert rod-shaped bacteria, which believed invade neighboring initiate new rounds colonization. So far, however, these...
Efficient conditions for the synthesis of tetra-, penta-, and hexasubstituted derivatives 1,4-dihydropyrrolo[3,2-b]pyrrole were developed. The tetraaryl obtained in a novel one-pot reaction among aromatic aldehydes, amines, butane-2,3-dione. After thorough examination various parameters (solvent, acid, temperature) p-toluenesulfonic acid was identified as crucial catalyst. As result, 1,4-dihydropyrrolo[3,2-b]pyrroles highest yields reported to date. scope limitation studies showed that this...
Topical therapies for chronic skin diseases suffer from a low patient compliance due to the inconvenient treatment regimens of available products. Dissolvable microneedles (MN) with modified release offer an interesting possibility increase by acting as depot in and thereby decreasing dosing frequency. Furthermore, bioavailability can be increased significantly bypassing barrier direct penetration MN into skin. In this study effect innovative dissolvable patch was assessed insertion ex vivo...
We investigate the texture of gel (g) domains in binary lipid membranes composed phospholipids DPPC and DOPC. Lateral organization bilayer is a topic fundamental biological importance. Whereas questions related to size composition fluid membrane domain are well studied, possibility has so far not been examined. When using polarized light for two-photon excitation fluorescent probe Laurdan, emission intensity highly sensitive angle between polarization tilt orientation acyl chains. By imaging...
In this study we use the combination of super resolution optical microscopy and raster image correlation spectroscopy (RICS) to mechanism action liposomes as transdermal drug delivery systems in human skin. Two different compositions were applied newly excised skin, a POPC liposome more flexible containing surfactant sodium cholate. Stimulated emission depletion (STED) images intact skin cryo-sections treated with labeled recorded displaying an low enough resolve 100 nm The revealed that...
Superfast muscles (SFMs) are extremely fast synchronous capable of contraction rates up to 250 Hz, enabling precise motor execution at the millisecond time scale. SFM phenotypes have been discovered in most major vertebrate lineages, but it remains unknown whether all SFMs share excitation-contraction coupling pathway adaptations for speed, and if arose once, or from independent evolutionary events. Here, we demonstrate that achieve rapid actomyosin crossbridge kinetics bat songbird express...
Cellular engulfment and uptake of macromolecular assemblies or nanoparticles via endocytosis can be associated to both healthy disease-related biological processes as well delivery drug potential nanotoxicity pollutants. Depending on the physical chemical properties system, adsorbed particles may remain at membrane surface, become wrapped by membrane, translocate across through an endocytosis-like process. In this paper, we address question how wrapping colloidal lipid membranes controlled...
Synapses are essential for neuronal function and central to numerous neurological disorders including developmental neurodegenerative diseases. structurally constitute a very small proportion of neuron, their protein content is difficult study using whole tissue preparations. Especially studying synapses on the functional level challenging. To overcome this limitation, can be captured as synaptosomes generated through enrichment isolated nerve terminals. Such have re-sealed plasma membrane...
Abstract Background Despite promising preclinical studies, the application of DNA methyltransferase inhibitors in treating patients with solid cancers has thus far produced only modest outcomes. The presence intratumoral heterogeneity response to could significantly influence clinical efficacy, yet our understanding single-cell these drugs tumors remains very limited. Methods In this study, we used cancer/testis antigen genes as a model for methylation-dependent gene expression examine...
Image resolution and field of view in far-field optical microscopy are often inversely proportional to one another due digital sampling limitations imposed by the magnification system pixel size sensor. We present a method including spatial shifting mechanism reconstruction algorithm that bypasses this trade-off sample be imaged subpixel increments, before registering images via phase correlation combining resulting registered using shift-and-add approach. Importantly, requires no specific...
Genome replication in eukaryotic cells is surveyed by the S-phase checkpoint, which orchestrates sequential origin activation to avoid exhaustion of hitherto poorly defined rate-limiting replisome components. Here, we find that excessive origins depletes chromatin-bound PCNA and lagging strand components, thereby limiting additional loading at new when checkpoint control disrupted. PAF15 (PCNA-associated factor 15) emerges as a dosage-sensitive regulator PCNA, delineating dynamic range...
Nanoplastics (NPs) pose potential ecological and health impacts. While previous studies have highlighted inconsistent toxicity levels of NPs, knowledge remains limited about the specific effects different NPs on embryonic development, early life-stage behaviors, bodily uptake. This study examines polystyrene (PS-NPs) with surface charges, plain (PS), amino-modified (PS-NH2), carboxyl-modified (PS-COOH) zebrafish life stages. High-resolution 3D bioimaging confirmed differential...