A5034624283- Advancements in Transdermal Drug Delivery
- Lipid Membrane Structure and Behavior
- Skin and Cellular Biology Research
- Skin Diseases and Diabetes
- Polysaccharides and Plant Cell Walls
- Autoimmune Bullous Skin Diseases
- Advanced Cellulose Research Studies
- Sphingolipid Metabolism and Signaling
- Cell Image Analysis Techniques
- Medical Imaging Techniques and Applications
- Surfactants and Colloidal Systems
- Advanced Electron Microscopy Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Computational Physics and Python Applications
- Plant nutrient uptake and metabolism
- Dermatology and Skin Diseases
Karolinska Institutet
2012-2020
Wageningen University & Research
2012
The skin barrier is fundamental to terrestrial life and its evolution; it upholds homeostasis protects against the environment. Skin capacity controlled by lipids that fill extracellular space of skin's surface layer--the stratum corneum. Here we report on determination molecular organization lipid matrix in situ, near-native state, using a methodological approach combining very high magnification cryo-electron microscopy (EM) vitreous section defocus series, modeling, EM simulation. are...
In vertebrates, skin upholds homeostasis by preventing body water loss. The skin's permeability barrier is located intercellularly in the stratum corneum and consists of stacked lipid lamellae composed ceramides, cholesterol, free fatty acids. We have combined cryo-electron microscopy with molecular dynamics modeling electron simulation our analysis lamellae's formation, a maturation process beginning granulosum ending corneum. Previously, we revealed initial- end-stage organizations. this...
Summary Cellulose is the most abundant biopolymer on earth, and has qualities that make it suitable for biofuel. There are new tools visualisation of cellulose synthase complexes in living cells, but those do not show their product, microfibrils (CMFs). In this study we report characteristics cell wall textures, i.e. architectures CMFs wall, root hairs Arabidopsis thaliana , Medicago truncatula Vicia sativa compare different techniques used to them. Root these species have a random primary...
The skin's permeability barrier consists of stacked lipid sheets splayed ceramides, cholesterol and free fatty acids, positioned intercellularly in the stratum corneum. We report here on early stage skin formation taking place inside tubuloreticular system secretory cells topmost viable epidermis intercellular space between process was analysed situ its near-native state, using cryo-EM combined with molecular dynamics modeling EM simulation. Stacks lamellae appear towards periphery they are...
Improved knowledge of the topology lamellar bodies is a prerequisite for molecular-level understanding skin barrier formation, which in turn may provide clues as to underlying causes barrier-deficient disease. The aim this study was examine key question continuity vs. discreteness body system using 3 highly specialized and complementary 3-dimensional (3D) electron microscopy methodologies; tomography vitreous sections (TOVIS), freeze-substitution serial section (FS-SET), focused ion beam...
Visual understanding is a key aspect when studying electron tomography data-sets, aside quantitative assessments such as registration of high-resolution structures. We here present the free software tool ProViz (Protein Visualization) for visualisation and template matching in tomograms biological samples. The contains methods tools which we have developed, adapted computationally optimised easy intuitive analysis with low signal-to-noise ratio. complements existing application field serves...