A5082538035- Advanced Electron Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Genetics and Biotechnology
- Electron and X-Ray Spectroscopy Techniques
- RNA modifications and cancer
- Force Microscopy Techniques and Applications
- Medical Imaging Techniques and Applications
- Chemical Thermodynamics and Molecular Structure
- Organoselenium and organotellurium chemistry
- RNA Research and Splicing
- Sparse and Compressive Sensing Techniques
- Advanced MRI Techniques and Applications
- Advanced X-ray Imaging Techniques
- Solid-state spectroscopy and crystallography
- Thermal and Kinetic Analysis
- Crystal structures of chemical compounds
- Nuclear Structure and Function
- Advanced Fluorescence Microscopy Techniques
- Organometallic Compounds Synthesis and Characterization
- Protist diversity and phylogeny
- Organic Chemistry Cycloaddition Reactions
- Molecular Spectroscopy and Structure
- Photosynthetic Processes and Mechanisms
Okinawa Institute of Science and Technology Graduate University
2013-2023
Karolinska Institutet
1998-2009
Van Andel Institute
2006
École Polytechnique Fédérale de Lausanne
2004
Columbia University
2004
Uppsala University
1982-1987
University of California, San Diego
1982
Stockholm University
1979-1982
Institute of Biophysics
1981
City of Hope
1981
The skin barrier is fundamental to terrestrial life and its evolution; it upholds homeostasis protects against the environment. Skin capacity controlled by lipids that fill extracellular space of skin's surface layer--the stratum corneum. Here we report on determination molecular organization lipid matrix in situ, near-native state, using a methodological approach combining very high magnification cryo-electron microscopy (EM) vitreous section defocus series, modeling, EM simulation. are...
Nephrin is a key functional component of the slit diaphragm, structurally unresolved molecular filter in renal glomerular capillaries. Abnormal nephrin or its absence results severe proteinuria and loss diaphragm. The diaphragm thin extracellular membrane spanning approximately 40-nm-wide filtration between podocyte foot processes covering capillary surface. Using electron tomography, we show that comprises network winding strands with pores same size as smaller than albumin molecules,...
The polypeptide growth factor, hepatocyte factor/scatter factor (HGF/SF), shares the multidomain structure and proteolytic mechanism of activation plasminogen other complex serine proteinases. HGF/SF, however, has no enzymatic activity. Instead, it controls growth, morphogenesis, or migration epithelial, endothelial, muscle progenitor cells through receptor tyrosine kinase MET. Using small-angle x-ray scattering cryo-electron microscopy, we show that conversion pro(single-chain)HGF/SF into...
Plasmodium falciparum virulence is associated with sequestration of infected erythrocytes. Microvascular binding mediated by PfEMP1 in complex non-immune immunoglobulin M (IgM) common among parasites that cause both severe childhood malaria and pregnancy-associated malaria. Here, we present cryo-molecular electron tomography structures human IgM, their complex. Three-dimensional reconstructions IgM reveal it has a dome-like core, randomly oriented Fab2s units, the overall shape turtle. C-...
Cell adhesion molecules (CAMs) sense the extracellular microenvironment and transmit signals to intracellular compartment. In this investigation, we addressed mechanism of signal generation by ectodomains single-pass transmembrane homophilic CAMs. We analyzed structure interactions carcinoembryonic antigen (CEA)–related CAM 1 (CEACAM1), which regulates cell proliferation, apoptosis, motility, morphogenesis, microbial responses. Soluble membrane-attached CEACAM1 were investigated surface...
Summary The division of Escherichia coli is mediated by a collection some 34 different proteins that are recruited to the septum and thought assemble into macromolecular complex known as ‘the divisome’. Herein, we have endeavored better understand structure divisome imaging two its core components; FtsZ FtsN. Super resolution microscopy (SIM gSTED) indicated both localized in large assemblies, which distributed around (i.e., forming discontinuous ring). Although rings had similar radii prior...
The divisome is the macromolecular complex that carries out cell division in Escherichia coli. Every generation it must be assembled, and then disassembled so sequestered proteins can recycled. Whilst assembly process has been well studied, virtually nothing known about disassembly process. In this study, we have used super-resolution SIM imaging to monitor pairs of fluorescently tagged as they depart from septum. These simple binary comparisons indicated occurs a coordinated consists at...
Sparsity exploiting image reconstruction (SER) methods have been extensively used with total variation (TV) regularization for tomographic reconstructions. Local TV fail to preserve texture details and often create additional artifacts due over-smoothing. Nonlocal (NLTV) proposed as a solution this but they either lack continuous updates computational constraints or limit the locality small region. In letter, we propose adaptive graph-based TV. The method goes beyond spatial similarity...
The issue of protein dynamics and its implications in the biological function proteins are arousing greater interest different fields molecular biology. In cryo-electron tomography experiments one may take several snapshots a given macromolecule. principle, large enough collection molecule then be used to calculate equilibrium configuration terms experimentally accessible degrees freedom and, hence, estimate potential energy. This information would crucial order analyze functions...
The core of late states maturing human immunodeficiency virus type 1 (HIV-1) has been visualized in three dimensions at approximately 7 nm resolution by electron microscopic tomography. After budding, 25 thick precursor material is observed densely assembled inside the viral envelope. Upon proteolysis transported and condensed center virion. core, 100 length, spans entire diameter virion showing a 40-60 wide free end narrow 20 nm. A model derived consisting two fibers packed into bilateral,...
Abstract The source of constriction required for division a bacterial cell remains enigmatic. FtsZ is widely believed to be key player, because in vitro experiments indicate that it can deform liposomes when membrane tethered. However vivo evidence such role has remained elusive as been challenging distinguish the contribution from peptidoglycan-ingrowth. To differentiate between these two possibilities we studied early stages Escherichia coli , present at site but peptidoglycan synthesizing...
Using electron tomography, we have analyzed whether the Balbiani ring (BR) pre-mRNP particles in transit from gene to nuclear pore complex (NPC) are bound any structure that could impair free diffusion through nucleoplasm. We show one-third of BR contact with thin connecting fibers (CFs), which some cases merge into large fibrogranular clusters. The CFs a specific protein composition different particles, as shown by immuno-EM. Moreover, identified hrp65 one components CFs. sequencing cDNA...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCrystal structure of ammonium samarium sulfate tetrahydrateBirgitta. Eriksson, Lars O. Larsson, Lauri. Niinisto, and Ulf. SkoglundCite this: Inorg. Chem. 1974, 13, 2, 290–295Publication Date (Print):February 1, 1974Publication History Published online1 May 2002Published inissue 1 February 1974https://pubs.acs.org/doi/10.1021/ic50132a008https://doi.org/10.1021/ic50132a008research-articleACS PublicationsRequest reuse permissionsArticle...
FtsZ is the main regulator of bacterial cell division. It has been implicated in acting as a scaffolding protein for other division proteins, force generator during constriction, and more recently, an active septal wall production. assembles into heterogeneous structure coined Z-ring due to its resemblance ring confined by midcell geometry. Here, establish framework examining geometrical influences on proper assembly dynamics, we sculpted Escherichia coli cells unnatural shapes using...
The transport of Balbiani ring (BR) premessenger RNP particles in the larval salivary gland cells dipteran Chironomus tentans can be followed using electron microscopy. A BR particle consists an ribbon bent into a ringlike structure. Upon translocation through nuclear pore complex (NPC), is straightened and enters central channel NPC with 5' end transcript lead. translocating likely to interact but, addition, remaining portion makes contact periphery NPC. To determine nature this latter...