A5009297306- Peptidase Inhibition and Analysis
- Radiopharmaceutical Chemistry and Applications
- Antifungal resistance and susceptibility
- Cell Adhesion Molecules Research
- Pneumonia and Respiratory Infections
- Fungal Infections and Studies
- Monoclonal and Polyclonal Antibodies Research
- Synthesis and Biological Evaluation
- Helicobacter pylori-related gastroenterology studies
- Glycosylation and Glycoproteins Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Microtubule and mitosis dynamics
- Coccidia and coccidiosis research
- Galectins and Cancer Biology
- Immune Response and Inflammation
- Veterinary medicine and infectious diseases
- Mitochondrial Function and Pathology
- Inhalation and Respiratory Drug Delivery
- Gut microbiota and health
- Tryptophan and brain disorders
- Immune cells in cancer
- DNA Repair Mechanisms
- Respiratory and Cough-Related Research
- Chemical Synthesis and Analysis
- Ubiquitin and proteasome pathways
Jena University Hospital
2013-2023
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2013
Friedrich Schiller University Jena
2013
Karolinska Institutet
2005-2010
Charité - Universitätsmedizin Berlin
2005-2007
ABSTRACT The C-type lectin receptor Dectin-1 is expressed mainly on myeloid cells mediating the immune response targeting respiratory pathogens such as Aspergillus fumigatus and Mycobacterium tuberculosis . pulmonary epithelium serves an important interface for interactions between these tract. Therefore, we analyzed expression pattern of in human lung. Immunohistochemically stained lung sections from 17 out 19 individuals were positive Dectin-1, which was apically bronchial alveolar...
Abstract Background The carcinoembryonic antigen (CEA)-related cell adhesion molecules CEACAM1 (BGP, CD66a), CEACAM5 (CEA, CD66e) and CEACAM6 (NCA, CD66c) are expressed in human lung. They play a role innate adaptive immunity targets for various bacterial viral adhesins. Two pathogens that colonize the normally sterile lower respiratory tract patients with chronic obstructive pulmonary disease (COPD) non-typable Haemophilus influenzae (NTHI) Moraxella catarrhalis . Both bind to CEACAMs...
Granulocytes form the first and fastest line of defense against pathogenic infections. Their survival is limited by apoptosis, a process that critical for resolution inflammation. Pro-apoptotic pro-inflammatory cytokines, as well several receptors, can alter lifespan granulocytes. Here we report carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1, CD66a) involved in regulation granulocyte survival. Until now CEACAM1 described to control proliferation, migration, tumor growth,...
Carcinoembryonic antigen (CEA)–related cell adhesion molecule 1 (CAM1 [CEACAM1]) mediates homophilic and regulates signaling. Although there is evidence that CEACAM1 binds activates SHP-1, SHP-2, c-Src, knowledge about the mechanism of transmembrane signaling lacking. To analyze regulation SHP-1/SHP-2/c-Src binding, we expressed various CFP/YFP-tagged isoforms in epithelial cells. The supramolecular organization was examined by cross-linking, coclustering, coimmunoprecipitation, fluorescence...
Cell adhesion molecules (CAMs) sense the extracellular microenvironment and transmit signals to intracellular compartment. In this investigation, we addressed mechanism of signal generation by ectodomains single-pass transmembrane homophilic CAMs. We analyzed structure interactions carcinoembryonic antigen (CEA)–related CAM 1 (CEACAM1), which regulates cell proliferation, apoptosis, motility, morphogenesis, microbial responses. Soluble membrane-attached CEACAM1 were investigated surface...
Candida albicans colonizes human mucosa, including the gastrointestinal tract, as a commensal. In immunocompromised patients, C. can breach intestinal epithelial barrier and cause fatal invasive infections. Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1; CD66a), CEACAM5 (CEA), CEACAM6 (CD66c) are immunomodulatory receptors expressed on mucosa recruited by bacterial viral pathogens. Here we show for first time that fungal pathogen (i.e., albicans) also binds directly to...
The human restricted pathogen Moraxella catarrhalis is an important causal agent for exacerbations in chronic obstructive lung disease adults. In such patients, increased numbers of granulocytes are present the airways, which correlate with bacteria-induced and severity disease. Our study investigated whether interaction M. granulocyte-specific carcinoembryonic antigen-related cell adhesion molecule (CEACAM)-3 linked to NF-κB activation, resulting chemokine production. Granulocytes from...
The carcinoembryonic antigen-related cell adhesion molecule CEACAM1 (CD66a) and the scaffolding protein filamin A have both been implicated in tumor migration. In present study we identified as a novel binding partner for CEACAM1-L cytoplasmic domain yeast two-hybrid screen. Direct was shown by surface plasmon resonance analysis affinity precipitation assays. association human rodent endogenous expressing cells. To address functional aspects of interaction, used well-established melanoma...
Beyond its well-documented role in reproduction, embryogenesis and maintenance of body tissues, vitamin A has attracted considerable attention due to immunomodulatory effects on both the innate adaptive immune responses. In infectious diseases, been shown have a host-protective effect infections bacterial, viral or protozoan origin. Nevertheless, impact fungal remains unknown. Meanwhile, frequency invasive mycoses keeps growing, with Candida albicans being major opportunistic pathogen...
The homophilic cell-cell adhesion receptor CEACAM1 (carcinoembryonic antigen-related cell molecule 1, CD66a) acts as a regulator of contact-dependent survival, differentiation, and growth. It is involved in the control proliferation hematopoietic epithelial cells can act tumor suppressor. In this study, we identify DNA polymerase delta-interacting protein 38 (PDIP38) novel binding partner for CEACAM1-L CEACAM1-S. We show that PDIP38 occur nucleus, cytoplasm at plasma membrane NBT-II, IEC18,...
In order to maintain genomic integrity during mitosis, cells assemble the mitotic spindle separate sister chromosomes two daughter cells. A variety of motor- and non motor-proteins are involved in organization regulation this complex apparatus. DNA polymerase delta-interacting protein 38 (PDIP38) is a highly conserved has so far been shown be cytoplasmic nuclear protein. Cell cycle dependent localization interaction with delta proliferating cell antigen (PCNA) indicate role for PDIP38...
In patients with chronic obstructive pulmonary disease (COPD), Moraxella catarrhalis infection of the lower airways is associated colonization and inflammation during stable acute exacerbations. Chronic smoke exposure induces impairs mucociliary clearance, thus contributing to bacterial in COPD patients. The human-specific carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 5, expressed human airways, has been shown contribute epithelial CEACAM-binding pathogens. To investigate...
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1, CD66a) is a receptor for Candida albicans. It crucial the immune response of intestinal epithelial cells to this opportunistic pathogen. Moreover, CEACAM1 importance mucosal colonization by different bacterial pathogens. We therefore studied influence human in CEACAM1-transgenic mice on C. albicans and infection utilizing colonization/dissemination systemic mouse model. Our results showed no alterations host between...
The C-type lectin receptor Dectin-1 is expressed mainly on myeloid cells mediating the immune response targeting respiratory pathogens such as Aspergillus fumigatus and Mycobacterium tuberculosis. pulmonary epithelium serves an important interface for interactions between these tract. Therefore, we analyzed expression pattern of in human lung. Immunohistochemically stained lung sections from 17 out 19 individuals were positive Dectin-1, which was apically bronchial alveolar epithelium. Our...
Invasive candidiasis is a healthcare-associated fungal infection with high mortality rate. Neutrophils, the first line of defense during infections, express immunoregulatory Candida albicans receptors CEACAM1, CEACAM3, and CEACAM6. We analyzed effects specific antibodies on C. albicans-induced neutrophil responses. CEACAM6 ligation by 1H7-4B to some extent CEACAM1 B3-17, but not CEACAM3 308/3-3, resulted in immediate release stored CXCL8 altered transcriptional responses albicans-stimulated...
Neutrophils are critical phagocytic cells in innate immunity, playing a significant role defending against invasive fungal pathogens. This study aimed to explore the transcriptional activation of human neutrophils response different pathogens, including Candida albicans and Aspergillus fumigatus, compared bacterial pathogen Escherichia coli. We identified distinct profiles stress-related pathways during infections, highlighting their functional diversity adaptability. The was largely...
Abstract Invasive candidiasis, often caused by Candida albicans , is an important healthcare-associated fungal infection that results in a high mortality rate of up to 40%. Neutrophils are the first line defense during infections. They can initiate various killing mechanisms and release cytokines attract further immune cells site infection. These responses tightly controlled, since they also lead severe tissue/organ damage. We hypothesized regulation C. -specific neutrophil functions...
The carcinoembryonic antigen (CEA)-related cell adhesion molecules CEACAM1 (BGP, CD66a), CEACAM5 (CEA, CD66e) and CEACAM6 (NCA, CD66c) are expressed in human lung. They play a role innate adaptive immunity targets for various bacterial viral adhesins. Two pathogens that colonize the normally sterile lower respiratory tract patients with chronic obstructive pulmonary disease (COPD) non-typable Haemophilus influenzae (NTHI) Moraxella catarrhalis. Both bind to CEACAMs elicit variety of cellular...