A5015415441- Platelet Disorders and Treatments
- Glycosylation and Glycoproteins Research
- Hemophilia Treatment and Research
- Blood groups and transfusion
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Blood Coagulation and Thrombosis Mechanisms
- Protein purification and stability
- Chronic Myeloid Leukemia Treatments
- Complement system in diseases
- Cell Adhesion Molecules Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Proteoglycans and glycosaminoglycans research
- Antiplatelet Therapy and Cardiovascular Diseases
- Advanced Proteomics Techniques and Applications
- Galectins and Cancer Biology
- Peptidase Inhibition and Analysis
- Heparin-Induced Thrombocytopenia and Thrombosis
- Viral Infectious Diseases and Gene Expression in Insects
- Adenosine and Purinergic Signaling
- Protease and Inhibitor Mechanisms
- Enzyme Production and Characterization
- Drug Transport and Resistance Mechanisms
- Neuroblastoma Research and Treatments
- Chemical Synthesis and Analysis
CellAct Pharma (Germany)
2015-2020
Mundipharma (Germany)
2019
Freie Universität Berlin
1992-2008
Octapharma (United States)
2008
Octapharma (Austria)
2002-2007
Charité - Universitätsmedizin Berlin
2005
Institute of Molecular Biology
1999-2002
DKFZ-ZMBH Alliance
1991
German Cancer Research Center
1991
In this study it could be shown that in rat the normally occurring N-acetyl neuraminic acid can modified its N-acyl moiety by vivo administration of chemically synthesized N-propanoyl precursors, N-propanoyl-D-glucosamine or N-propanoyl-D-mannosamine. It each these nonphysiological amino sugar analogues was incorporated into both membrane and serum glycoproteins. After treatment rats with radiolabeled N-[acyl-1-14C]D-mannosamine, radioactivity removed from glycoprotein fractions incubation...
MS-1, a high-molecular-mass protein expressed by non-continuous and angiogenic endothelial cells alternatively activated macrophages (Mφ2), the hepatic sinusoidal hyaluronan clearance receptor are similar with respect to tissue distribution biochemical characteristics. In present study we purified these proteins immuno- hyaluronan-affinity chromatography respectively, sequenced tryptic peptides generated full-length cDNA sequences in both mouse human. The novel genes, i.e. stabilin-1...
MS-1, a high-molecular-mass protein expressed by non-continuous and angiogenic endothelial cells alternatively activated macrophages (Mφ2), the hepatic sinusoidal hyaluronan clearance receptor are similar with respect to tissue distribution biochemical characteristics. In present study we purified these proteins immuno- hyaluronan-affinity chromatography respectively, sequenced tryptic peptides generated full-length cDNA sequences in both mouse human. The novel genes, i.e. stabilin-1...
Direct photoaffinity labeling of liver plasma membrane subfractions enriched in sinusoidal and canalicular membranes using [35S]adenosine 5'-O-(thiotriphosphate) ([35S]ATP gamma S) allows the identification ATP-binding proteins these domains. Comparative with [35S]ATP S photolabile bile salt derivative (7,7-azo-3 alpha, 12 alpha-dihydroxy-5 beta-[3 beta-3H]-cholan-24-oyl-2'- aminoethanesulfonate followed by immunoprecipitation a monoclonal antibody (Be 9.2) revealed identity salt-binding...
Sialylation is essential for development and regeneration in mammals. Using N-propanoylmannosamine, a novel precursor of sialic acid, we were able to incorporate unnatural acids with prolonged N-acyl side chain (e.g., N-propanoylneuraminic acid) into cell surface glycoconjugates. Here report that this biochemical engineering acid leads stimulation neuronal cells. Both PC12 cells cerebellar neurons showed significant increase neurite outgrowth after treatment precursor. Furthermore, also the...
The first meeting of international specialists in the field von Willebrand disease (VWD) was held Åland islands 1998 where Erik had observed a bleeding disorder some members family from Föglö and summary published 1999. second 2010 report 2012. Topics covered included progress understanding VWD over last 50 years; multimers; classification VWD; pharmacokinetics laboratory assays; genetics; treating paediatric patient; prophylaxis; geriatrics; gene therapy treatment guidelines. This third 3...
The carcinoembryonic antigen-related cell adhesion molecule CEACAM1 (CD66a) and the scaffolding protein filamin A have both been implicated in tumor migration. In present study we identified as a novel binding partner for CEACAM1-L cytoplasmic domain yeast two-hybrid screen. Direct was shown by surface plasmon resonance analysis affinity precipitation assays. association human rodent endogenous expressing cells. To address functional aspects of interaction, used well-established melanoma...
Background Sialic acids (Sia) represent negative-charged terminal sugars on most glycoproteins and glycolipids the cell surface of vertebrates. Aberrant expression tumor associated sialylated carbohydrate epitopes significantly increases during onset cancer. Since Sia contribute towards migration ( = metastasis) to chemo- radiation resistance. Modulation cellular concentration composition poses a challenge especially for neuroblastoma therapy, due high heterogeneity therapeutic resistance...
The neural cell adhesion molecule (NCAM) is implicated in important functions during development and maintenance of the nervous system. Two three major isoforms, NCAM 140 180, are transmembrane glycoproteins with large cytoplasmic domains different length. purpose this study was to identify novel intracellular binding partners 180. We expressed both domains, as well fragments NCAM, fusion proteins Escherichia coli used them for ligand affinity chromatography or glutathione S-transferase...
The homophilic cell-cell adhesion receptor CEACAM1 (carcinoembryonic antigen-related cell molecule 1, CD66a) acts as a regulator of contact-dependent survival, differentiation, and growth. It is involved in the control proliferation hematopoietic epithelial cells can act tumor suppressor. In this study, we identify DNA polymerase delta-interacting protein 38 (PDIP38) novel binding partner for CEACAM1-L CEACAM1-S. We show that PDIP38 occur nucleus, cytoplasm at plasma membrane NBT-II, IEC18,...
Due to its position at the outermost of glycans, sialic acid is involved in a myriad physiological and pathophysiological cell functions such as host-pathogen interactions, immune regulation, tumor evasion. Inhibitors surface sialylation could be useful tool cancer, immune, antibiotic, or antiviral therapy. In this work, four different C-3 modified N-acetylmannosamine analogs were tested potential inhibitors sialylation. Peracetylated 2-acetylamino-2-deoxy-3-O-methyl-D-mannose decreases...
Human-cl rhFVIII (Nuwiq®), a new generation recombinant factor VIII (rFVIII), is the first rFVIII produced in human cell-line approved by European Medicines Agency. To describe development, upscaling and process validation for industrial-scale human-cl purification. The purification involves one centrifugation, two filtration, five chromatography columns dedicated pathogen clearance steps (solvent/detergent treatment 20 nm nanofiltration). key step uses an affinity resin (VIIISelect) with...
The pathomechanism of thrombotic thrombocytopenic purpura (TTP) and atypical haemolytic uraemic syndrome (aHUS) is associated with a severe deficiency ADAMTS13 factor H. aim this study was to quantify the levels H in pharmaceutically licensed plasma for transfusion, Octaplas, universally applicable plasma, Uniplas (development product, working title). Furthermore, Octaplas batches blood groups A, B, O, AB, plasmas derived from different sources were compared.Twenty-four three selected study....