A5022651422- Advancements in Transdermal Drug Delivery
- Lipid Membrane Structure and Behavior
- Dermatology and Skin Diseases
- Surfactants and Colloidal Systems
- Skin and Cellular Biology Research
- Advanced Electron Microscopy Techniques and Applications
- Nail Diseases and Treatments
- Essential Oils and Antimicrobial Activity
- Advanced Fluorescence Microscopy Techniques
- Contact Dermatitis and Allergies
- Toxin Mechanisms and Immunotoxins
- Dyeing and Modifying Textile Fibers
- Skin Protection and Aging
- Textile materials and evaluations
- Skin Diseases and Diabetes
- Electron and X-Ray Spectroscopy Techniques
- RNA regulation and disease
- Bee Products Chemical Analysis
- Proteins in Food Systems
- Hair Growth and Disorders
- Photosynthetic Processes and Mechanisms
- Dermatological and Skeletal Disorders
- Neonatal skin health care
- Sphingolipid Metabolism and Signaling
- Autoimmune Bullous Skin Diseases
Karolinska Institutet
2015-2024
Karolinska University Hospital
2014-2024
Nobel Foundation
2008
University of Geneva
2001-2005
The skin barrier is fundamental to terrestrial life and its evolution; it upholds homeostasis protects against the environment. Skin capacity controlled by lipids that fill extracellular space of skin's surface layer--the stratum corneum. Here we report on determination molecular organization lipid matrix in situ, near-native state, using a methodological approach combining very high magnification cryo-electron microscopy (EM) vitreous section defocus series, modeling, EM simulation. are...
Very little is known about the in vivo regulation of mammalian fatty acid chain elongation enzymes as well role specific length cellular responses and developmental processes. Here, we report that Elovl3 gene product, which belongs to a highly conserved family microsomal involved formation very long acids, revealed distinct expression skin was restricted sebaceous glands epithelial cells hair follicles. By disruption by homologous recombination mouse, show ELOVL3 participates neutral lipids...
Understanding and predicting permeability of compounds through skin is interest for transdermal delivery drugs toxicity predictions chemicals. We show, using a new atomistic molecular dynamics model the skin's barrier structure, itself validated against near-native cryo-electron microscopy data from human skin, that to reference benzene, DMSO (dimethyl sulfoxide), ethanol, codeine, naproxen, nicotine, testosterone water can be predicted. The results were in literature. have investigated...
The introduction of unclassified new psychoactive substances (NPS) on the recreational drugs market through open online sale ('legal highs' or 'Internet drugs') continues unabated and represents a growing health hazard. use NPS has resulted in numerous, severe, adverse events fatalities, due to unintended overdose unknown toxic side‐effects. To try find possible common underlying cause for skin–hair–eye symptoms complex observed three men. From late 2013 mid‐2014, Swedish men aged 23–34...
In the present study we have analyzed molecular structure and function of human skin's permeability barrier using dynamics simulation validated against cryo-electron microscopy data from near native skin. The capacity is located to an intercellular lipid embedding cells superficial most layer skin - stratum corneum. According splayed bilayer model (Iwai et al., 2012) organized as stacked bilayers ceramides in a chain conformation with cholesterol associated ceramide sphingoid moiety free...
In vertebrates, skin upholds homeostasis by preventing body water loss. The skin's permeability barrier is located intercellularly in the stratum corneum and consists of stacked lipid lamellae composed ceramides, cholesterol, free fatty acids. We have combined cryo-electron microscopy with molecular dynamics modeling electron simulation our analysis lamellae's formation, a maturation process beginning granulosum ending corneum. Previously, we revealed initial- end-stage organizations. this...
Our skin constitutes an effective permeability barrier that protects the body from exogenous substances but concomitantly severely limits number of pharmaceutical drugs can be delivered transdermally. In topical formulation design, chemical permeation enhancers (PEs) are used to increase drug permeability. vitro experiments measure net effects PEs on transdermal transport, they cannot explain molecular mechanisms interactions between drugs, enhancers, and structure, which possibility...
It was recently shown that the stratum corneum fat layer, i.e. skin permeability barrier, has a unique molecular arrangement - stacked bilayers of fully extended ceramides with cholesterol molecules associated ceramide sphingoid moiety. We here give brief historical perspective to field barrier research and specifically address some selected contributions current understanding morphology.
The skin's permeability barrier consists of stacked lipid sheets splayed ceramides, cholesterol and free fatty acids, positioned intercellularly in the stratum corneum. We report here on early stage skin formation taking place inside tubuloreticular system secretory cells topmost viable epidermis intercellular space between process was analysed situ its near-native state, using cryo-EM combined with molecular dynamics modeling EM simulation. Stacks lamellae appear towards periphery they are...