A5033913039- Congenital heart defects research
- Advanced Fluorescence Microscopy Techniques
- Physiological and biochemical adaptations
- Optical Imaging and Spectroscopy Techniques
- CCD and CMOS Imaging Sensors
- Zebrafish Biomedical Research Applications
- Optical Coherence Tomography Applications
- Cardiac Imaging and Diagnostics
- Medical Imaging Techniques and Applications
- Electrochemical Analysis and Applications
- Cardiomyopathy and Myosin Studies
- Advanced Electron Microscopy Techniques and Applications
- Advanced MRI Techniques and Applications
- Coronary Artery Anomalies
- Cell Image Analysis Techniques
The University of Texas at Dallas
2023-2024
Image resolution and field of view in far-field optical microscopy are often inversely proportional to one another due digital sampling limitations imposed by the magnification system pixel size sensor. We present a method including spatial shifting mechanism reconstruction algorithm that bypasses this trade-off sample be imaged subpixel increments, before registering images via phase correlation combining resulting registered using shift-and-add approach. Importantly, requires no specific...
Zebrafish is an intriguing model organism known for its remarkable cardiac regeneration capacity. Studying the contracting heart in vivo essential gaining insights into structural and functional changes response to injuries. However, obtaining high-resolution high-speed 4-dimensional (4D, 3D spatial + 1D temporal) images of zebrafish assess architecture contractility remains challenging. In this context, in-house light-sheet microscope (LSM) customized computational analysis are used...
Despite ongoing efforts in cardiovascular research, the acquisition of high-resolution and high-speed images for purpose assessing cardiac contraction remains challenging. Light-sheet fluorescence microscopy (LSFM) offers superior spatiotemporal resolution minimal photodamage, providing an indispensable opportunity
Light-sheet microscopy (LSM) enables us to strengthen the understanding of cardiac development, injury, and regeneration in mammalian models. This emerging technique decouples laser illumination fluorescence detection investigate micro-structure function with a high spatial resolution while minimizing photodamage maximizing penetration depth. To unravel potential volumetric imaging development repair, we sought integrate our in-house LSM, Adipo-Clear, virtual reality (VR) neonatal mouse...
Light-sheet microscopy (LSM) plays a pivotal role in comprehending the intricate three-dimensional (3D) structure of heart, providing crucial insights into fundamental cardiac physiology and pathologic responses. We hereby delve development implementation LSM technique to elucidate micro-architecture heart mouse models. The methodology integrates customized system with tissue clearing techniques, mitigating light scattering within tissues for volumetric imaging. combination conventional...
Paroxysmal arrhythmias caused by medications are challenging to be prospectively identified. Zebrafish have emerged as an ideal model organism for screening small molecule compounds study cardiac abnormalities, due their rapid development, optical transparency during early stages, and similarities the human heart. To overcome challenges associated with observing abnormalities in zebrafish, we sought develop a light-field microscope, imaging method high photon efficiency, volumetric...
The neonatal mouse heart in contrast to the adults has ability regenerate after myocardial infarction. investigation of cardiac morphogenesis is critical uncover underlying mechanism regeneration. Hence, we have developed a light-sheet microscope (LSM) along with tissue-clearing method investigate 3-dimensional (3D) architecture intact heart. We improved our imaging system by incorporating an axially swept remote focusing arm voice coil actuator for isotropic imaging. Our LSM offers lateral...
Light-sheet microscopy (LSM) plays a pivotal role in comprehending the intricate three-dimensional (3D) structure of heart, providing crucial insights into fundamental cardiac physiology and pathologic responses. We hereby delve development implementation LSM technique to elucidate micro-architecture heart mouse models. The methodology integrates customized system with tissue clearing techniques, mitigating light scattering within tissues for volumetric imaging. combination conventional...
Advanced understanding of cardiac structure and function is crucial for uncovering the underlying mechanism injury arrhythmias. To explore cues to structural functional abnormalities in extensively established animal models, we have developed optical imaging computational methods tailored investigate intact hearts zebrafish rodents at cellular resolution. Our light-field microscopy allows us capture instantaneous dynamics such as calcium transients, irregular contraction, blood flow 200...
Despite advancements in cardiovascular engineering, heart diseases remain a leading cause of mortality. The limited understanding the underlying mechanisms cardiac dysfunction at cellular level restricts development effective screening and therapeutic methods. To address this, we have developed framework that incorporates light field detection individual cell tracking to capture real-time volumetric data zebrafish hearts, which share structural electrical similarities with human generate 120...
Heart failure remains the leading cause of mortality worldwide, largely due to lack regenerative capacity mature cardiomyocytes. The advanced understanding cardiac architecture and function is critical uncover underlying mechanism morphogenesis remodeling process in response myocardial infarction. Recent progress has demonstrated that zebrafish neonatal mice have restore following injury, laying foundation investigate cues structural functional abnormalities. For this reason, we developed a...