A5004351930- Lipid Membrane Structure and Behavior
- S100 Proteins and Annexins
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Cell Adhesion Molecules Research
- Phagocytosis and Immune Regulation
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Hereditary Neurological Disorders
- Complement system in diseases
- Respiratory viral infections research
- Endoplasmic Reticulum Stress and Disease
- Neurological diseases and metabolism
- Spectroscopy and Quantum Chemical Studies
- Surfactants and Colloidal Systems
- Connexins and lens biology
- Immunotherapy and Immune Responses
- Elasticity and Material Modeling
- Pneumonia and Respiratory Infections
- Neonatal Respiratory Health Research
- Phenothiazines and Benzothiazines Synthesis and Activities
- Escherichia coli research studies
- Pharmacological Receptor Mechanisms and Effects
Institut Cochin
2018-2025
Université Paris Cité
2019-2025
Inserm
2018-2025
Centre National de la Recherche Scientifique
2019-2025
University of Southern Denmark
2020-2024
The University of Melbourne
2015-2022
Peter Doherty Institute
2015-2022
Sorbonne Paris Cité
2020
Florey Institute of Neuroscience and Mental Health
2015
Despite the importance of encapsulation in bacterial pathogenesis, biochemical mechanisms and forces that underpin retention capsule by encapsulated bacteria are poorly understood. In Gram-negative bacteria, there may be interactions between lipopolysaccharide (LPS) core polymers, polymers with retained acyl carriers outer membrane, some Wzi, an membrane protein lectin. Our transposon studies Klebsiella pneumoniae B5055 identified additional genes that, when insertionally inactivated,...
Increasing rates of antimicrobial-resistant medically important bacteria require the development new, effective therapeutics, which antimicrobial peptides (AMPs) are among promising candidates. Many AMPs membrane-active, but their mode action in killing or inhibiting growth remains elusive. This study used atomic force microscopy (AFM) to probe mechanobiology a model AMP (a derivative melittin) on living Klebsiella pneumoniae bacterial cells. We performed situ biophysical measurements...
The proper formation and function of the myelin sheath, a proteolipid membrane multilayer, relies on coordinated action several key proteins. We studied how proteins from peripheral cytoplasmic apposition—myelin basic protein (MBP), tail zero (P0ct), 2 (P2)—interact with each other myelin‐like membranes using various techniques, such as small‐angle X‐ray diffraction, differential scanning calorimetry (DSC), surface plasmon resonance (SPR), electron live epifluorescence microscopy. DSC...
Repair of damaged plasma membrane in eukaryotic cells is largely dependent on the binding annexin repair proteins to phospholipids. Changing biophysical properties may provide means compromise annexin-mediated and sensitize injury. Since, cancer experience heightened stress are more efficient repair, inhibiting approaches damage cell death. Here, we show that derivatives phenothiazines, which have widespread use fields psychiatry allergy treatment, strongly mechanical-, chemical-,...
Rapid membrane repair is required to ensure cell survival after rupture of the plasma membrane. The annexin family proteins involved in (PMR) and activated by influx Ca
The major myelin protein expressed by the peripheral nervous system Schwann cells is zero (P0), which represents 50% of total content in myelin. This 30-kDa integral membrane consists an immunoglobulin (Ig)-like domain, a transmembrane helix, and 69-residue C-terminal cytoplasmic tail (P0ct). basic residues P0ct contribute to tight packing lipid bilayers, alterations affect how P0 functions as adhesion molecule necessary for stability compact Several neurodegenerative neuropathies are...
Abstract Efficient plasma membrane repair (PMR) is required to damage sustained in the cellular life cycle. The annexin family of proteins, involved PMR, are activated by Ca 2+ influx from extracellular media at site injury. Mechanistic studies annexins have been overwhelmingly performed using a single annexin, despite recruitment multiple sites living cells. Hence, we investigate effect presence crosslinking annexins, A1, A2 and A6 (ANXA1, ANXA2 ANXA6) on curvature induction A4 (ANXA4)...
Abstract The major myelin protein expressed by the peripheral nervous system Schwann cells is zero (P0), representing 50% of total content in myelin. This 30-kDa integral membrane consists an immunoglobulin (Ig)-like domain, a transmembrane helix, and 69-residue C-terminal cytoplasmic tail (P0ct). basic residues P0ct contribute to tight packing lipid bilayers, alterations affect how P0 functions as adhesion molecule necessary for stability compact Several neurodegenerative neuropathies are...
Antimicrobial peptides (AMPs) are promising therapeutic alternatives to conventional antibiotics. Many AMPs membrane-active but their mode of action in killing bacteria or inhibiting growth remains elusive. Recent studies indicate the mechanism depends on peptide structure and lipid components bacterial cell membrane. Owing complexity working with living cells, most these have been conducted synthetic membrane systems, which neglect possible role surface structures interactions. In recent...
Self-association of cholesterol into aggregates and crystals is a hallmark developing atherosclerosis. Intrinsically fluorescent sterols, such as dehydroergosterol (DHE), can be used to study sterol aggregation by fluorescence spectroscopy microscopy, but thorough understanding DHE's photophysical structural properties in the aggregated state missing. Here, we show that DHE forms submicron when evaporated from an ethanol solution. Using atomic force find DHE, like cholesterol, compact...
Abstract Myelin is a proteolipid membrane multilayer held together by set of proteins. The proper formation and function the myelin sheath relies on coordinated action several key Research exploring how proteins from peripheral cytoplasmic apposition – basic protein (MBP), tail zero (P0ct), 2 (P2) interact with each other myelin-like membranes was conducted using various techniques, such as small-angle X-ray diffraction (SAXD), differential scanning calorimetry (DSC), surface plasmon...
Phagocytosis is the mechanism of internalization large particles, microorganisms and cellular debris. The complement pathway represents one first mechanisms defense against infection receptor 3 (CR3), which highly expressed on macrophages, a major for many pathogens Key to dissecting by CR3-mediated phagocytosis occurs, understanding how complex actin binding protein machinery associated regulators interact with during phagocytosis, from triggering receptor, through phagosome formation...
In higher organisms, the professional phagocytes of immune system (dendritic cells, neutrophils, monocytes, and macrophages) are responsible for pathogen clearance, development responses via cytokine secretion presentation antigens derived from internalized material, normal turnover remodelling tissues disposal dead cells. These functions rely on ability to migrate adhere sites infection, dynamically probe their environments make contact with phagocytic targets, perform phagocytosis, a...